MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution
MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution leverages the high sensitivity and rapid analytical capability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to deliver high-throughput, accurate structural characterization of diverse glycan samples. In a single experiment, this approach enables both molecular weight determination and spectral acquisition, making it particularly suited for large-scale sample screening and preliminary structural identification. With rapid analysis speed, minimal sample consumption, and high data reproducibility, it is widely applicable in glycomics research, glycan-based drug development, glycosylation quality control, and biomarker discovery.
MtoZ Biolabs operates advanced MALDI-TOF-MS systems combined with multidimensional separation and pretreatment workflows. Through standardized procedures and rigorous data analysis strategies, we provide high-quality, reproducible glycan screening services for both academic and industrial applications.
Technical Principles
MALDI-TOF-MS is a soft ionization mass spectrometry technique in which the sample is co-crystallized with an appropriate matrix and irradiated with a pulsed laser to gently desorb and ionize the molecules. The resulting charged ions are separated in a flight tube according to their mass-to-charge ratio (m/z) before detection, generating the mass spectrum.
In glycan analysis, MALDI-TOF-MS offers key advantages:
· Soft ionization: Preserves native glycan structures and minimizes fragmentation.
· Rapid data acquisition: Captures full-spectrum information within seconds, ideal for high-throughput applications.
· Wide dynamic range: Enables detection of both high- and low-abundance glycans.
· High resolving power: Accurately differentiates glycan isomers of varying molecular weights.
When combined with derivatization methods such as permethylation or aromatic amine labeling, MALDI-TOF-MS further enhances glycan signal intensity and improves structural resolution.
Analysis Workflow
1. Sample Pretreatment and Enrichment
Glycans are released via enzymatic digestion or chemical cleavage, followed by impurity removal using solid-phase extraction or magnetic bead enrichment.
2. Derivatization (Optional)
Glycans may be methylated or fluorescently labeled to improve signal intensity and analytical resolution.
3. Matrix Co-crystallization
The sample is mixed with an appropriate MALDI matrix (such as DHB or CHCA) and spotted onto a target plate to form uniform crystals.
4. MALDI-TOF-MS Detection
Optimized laser energy conditions are applied to acquire high signal-to-noise glycan mass spectra.
5. Data Analysis and Structural Identification
Database searches, peak matching, and pattern recognition are performed for molecular weight determination and preliminary structural interpretation. LC-MS/MS can be incorporated for in-depth analysis when required.
Du, H. et al. Anal. Chem. 2019.
Figure 1. Workflow of MALDI-TOF-MS-Based High-Throughput Glycan Screening
Why Choose MtoZ Biolabs?
✅ Advanced Detection Platforms: High-resolution MALDI-TOF-MS systems with multiple matrix options for broad glycan coverage.
✅ High-Throughput Capability: Efficiently processes hundreds of samples for large-scale studies.
✅ Multidimensional Separation Strategies: Integrates liquid chromatography and magnetic bead enrichment to improve accuracy in complex matrices.
✅ Customizable Solutions: Detection protocols, data analysis workflows, and report formats tailored to specific research objectives.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
We accept a wide range of glycan-related samples, including purified glycans, glycoproteins, glycopeptides, and biological specimens such as blood, urine, and tissue. To ensure analytical accuracy and reproducibility, samples should be prepared according to standardized procedures with clearly documented origins. Detailed preparation and submission guidelines are available from MtoZ Biolabs upon request.
Applications
The MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution supports a wide range of research and industrial applications involving glycan profiling and quality assessment. Typical use cases include:
· Glycomics Research: Characterization of glycan diversity and distribution patterns across biological samples.
· Biomarker Discovery: Identification of disease-associated glycan structures with potential diagnostic value.
· Glycan Drug Development: Structural profiling and quality control of glycosylated drug candidates.
· Glycosylation Quality Monitoring: Assessment of batch-to-batch consistency and stability of glycosylation in biologics.
· Food and Environmental Analysis: Detection of carbohydrate components in functional foods or polysaccharides in environmental samples.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Mass Spectrum & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Optional Bioinformatics Insights
6. Raw Data Files
With cutting-edge mass spectrometry systems and an experienced technical team, MtoZ Biolabs’ MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution delivers efficient, precise support for glycan research. We provide reliable data and comprehensive structural interpretation to advance glycomics, drug development, and functional carbohydrate applications. Contact us to learn how this solution can accelerate your research.
FAQ
Q1: Is MALDI-TOF-MS suitable for all glycan analyses?
MALDI-TOF-MS is highly sensitive to medium- and high-molecular-weight glycans, making it ideal for rapid screening and qualitative profiling. For detailed structural elucidation of complex isomers, we recommend combining MALDI-TOF-MS with LC-MS/MS.
Related Services
High-Throughput Glycan Screening Solution
HPLC-Based High-Throughput Glycan Screening Solution
Automated LC-MS-Based High-Throughput Glycan Screening Solution
UHPLC-FLD-Based High-Throughput Glycan Screening Solution
Hydrophilic Interaction Chromatography-Based High-Throughput Glycan Screening Solution
How to order?
