UHPLC-FLD-Based High-Throughput Glycan Screening Solution

Glycans are structurally complex and functionally diverse biomolecules involved in key biological processes such as cell signaling, immune modulation, protein folding, pathogen recognition, and disease progression. As glycoscience continues to advance, there is a growing demand for high-throughput and reliable analytical tools to support research and development across life sciences, clinical diagnostics, and biopharmaceuticals.

 

Ultra-high-performance liquid chromatography with fluorescence detection (UHPLC-FLD) has emerged as a powerful technique for glycan analysis, offering excellent separation capability, fast run times, and high sensitivity. MtoZ Biolabs’ UHPLC-FLD-Based High-Throughput Glycan Screening Solution combines automated sample handling, high-resolution chromatography, and sensitive fluorescence detection with intelligent data processing, ensuring robust, reproducible, and efficient glycan profiling. This service delivers accurate, traceable results that support a wide range of glycomics applications.

 

Analysis Workflow

1. Sample Pretreatment and Glycan Release

Remove proteins and impurities, release glycans using enzymatic digestion or chemical methods, followed by fluorescent derivatization labeling.

 

2. Automated Injection and UHPLC Separation

Inject derivatized glycans into the UHPLC system and apply optimized separation parameters to achieve high-resolution separation of glycan components.

 

3. Fluorescence Detection and Signal Acquisition

The FLD captures the fluorescence signal from labeled glycans, ensuring high-sensitivity detection and consistent peak shape quality.

 

4. Data Analysis and Quantitative Calculation

Use glycan databases and retention time matching algorithms for component identification and relative quantitation.

 

5. Result Integration and Visualization

Deliver standardized chromatograms, glycan composition profiles, and cross-sample comparative analysis results.

 



Figure 1. Workflow of UHPLC-FLD-Based High-Throughput Glycan Screening

 

Sample Submission Suggestions

UHPLC-FLD-Based High-Throughput Glycan Screening Solution supports various biological sample types, including glycoproteins, glycolipids, blood, serum, urine, cell lysates, and tissue homogenates. Samples may be provided in liquid or lyophilized form. To ensure accuracy, avoid high-salt content, strong background fluorescence substances, or components with high UV absorbance that could interfere with detection.

 

For complex matrices or special sample types, it is recommended to consult with the MtoZ Biolabs technical team in advance. We will provide a detailed sample submission guide and a customized pretreatment plan to ensure scientific rigor and reproducibility.

 

Why Choose MtoZ Biolabs?

✅ High-Resolution, High-Sensitivity Platform: Combines efficient chromatographic separation with fluorescence detection for rapid and accurate analysis of complex glycan mixtures.

✅ High-Throughput Screening Capability: Supports automated analysis and data processing for large sample batches, reducing turnaround time.

✅ Optimized Derivatization Methods: Enhances signal intensity and detection sensitivity while preserving glycan structural integrity.

✅ Broad Sample Compatibility: Suitable for diverse biological matrices, meeting both research and industrial application needs.

✅ Expert Data Interpretation: Backed by comprehensive glycan standard libraries and advanced algorithmic tools to ensure interpretable, traceable, and application-ready data.

 

Applications

● Glycan Quality Control and Drug Analysis: Monitor glycosylation consistency and batch-to-batch variation in biopharmaceuticals.

● Glycan Biomarker Discovery: Identify characteristic glycan patterns associated with diseases or physiological states.

● Food and Nutrition Science: Analyze glycan composition and properties in foods and raw materials.

● New Material Development: Explore the potential of specific glycan modifications in functional materials.

● Glycoscience Mechanism Research: Investigate structural changes and functional roles of glycans under different biological conditions.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Fluorescence Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs’ UHPLC-FLD-Based High-Throughput Glycan Screening Solution combines ultra-efficient separation technology, sensitive fluorescence detection, and intelligent data processing to deliver efficient, precise, and large-scale glycan screening and quantitation. This solution meets the high standards required in research, clinical, pharmaceutical, and industrial applications, offering scientifically robust and directly applicable analytical data and technical support.


Contact MtoZ Biolabs to obtain a customized analytical plan and professional services tailored to your research and application needs.

 

FAQ

Q1: How does UHPLC-FLD detection sensitivity compare to HPLC?

UHPLC, combined with small-particle stationary phases and high-pressure systems, significantly improves peak resolution and separation efficiency. When paired with the high signal-to-noise ratio of FLD, it achieves lower limits of detection, suitable for trace-level glycan quantitation.

 

Q2: How capable is this solution in glycan structure analysis?

UHPLC-FLD provides key quantitative parameters such as retention time and peak area for each glycan and can infer glycan types by comparison with standards. However, for detailed analysis of unknown structures, multiple modifications, or isomers, high-resolution mass spectrometry (MS) remains an essential complement. MtoZ Biolabs can integrate UHPLC-FLD with MS to deliver a complete workflow from quantitative screening to structural elucidation.

 

Q3: What is the main difference between UHPLC-FLD and LC-MS?

UHPLC-FLD is better suited for high-throughput quantitative screening, with faster data processing and lower cost, while LC-MS excels in structural analysis and modification site localization. Using both in combination provides the most comprehensive information.

 

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