Mass Spectrometry-Based High-Throughput Glycan Screening Solution
Mass Spectrometry-Based High-Throughput Glycan Screening Solution is a highly efficient and accurate strategy for glycan profiling, widely applied in glycomics, glycobiology, drug discovery, and biomarker research. Leveraging advanced mass spectrometry platforms and high-throughput detection capabilities, MtoZ Biolabs provides rapid, reliable, and comprehensive glycan structural analysis, modification identification, and quantitative profiling across large sample cohorts. Our service empowers researchers and industry clients with high-quality data to accelerate both basic science and translational applications.
Services at MtoZ Biolabs
MtoZ Biolabs’ Mass Spectrometry-Based High-Throughput Glycan Screening Solution integrates Electrospray Ionization (ESI) and Matrix-Assisted Laser Desorption/Ionization (MALDI), ensuring efficient ionization of glycan molecules. High-resolution mass spectrometry precisely determines the mass-to-charge ratios of glycans, enabling confident identification.
With multi-stage tandem mass spectrometry (MSⁿ), glycans are sequentially fragmented to reveal monosaccharide composition, branching patterns, and linkage information. This comprehensive workflow ensures accurate mass determination and detailed structural elucidation, even for low-abundance glycans and complex mixtures in large-scale, high-throughput projects.
Analysis Workflow
1. Sample Preparation
Compatible with serum, plasma, urine, tissues, cell extracts, and other biological matrices. Pre-processed samples are prepared for MS analysis.
2. Pretreatment and Digestion
Protein removal and enzymatic digestion (e.g., trypsin) to obtain glycopeptides or glycan mixtures.
3. Labeling and Enrichment
Isotope labeling (iTRAQ, TMT) and lectin affinity enrichment strategies to enhance glycopeptide detection sensitivity.
4. Mass Spectrometry Analysis
Advanced LC-MS/MS and MSⁿ platforms enable high-resolution detection of glycans.
5. Data Interpretation
Database-assisted annotation and algorithm-based quantification provide accurate molecular weight and structure information.
6. Results Delivery
Comprehensive glycan composition, structural insights, and abundance data, supporting downstream biological or clinical studies.
Zhu, J. et al. MASS SPECTROM REV. 2018.
Figure 1. High-Throughput Glycan Screening by Mass Spectrometry
Why Choose MtoZ Biolabs?
✅ Exceptional Sensitivity and Specificity: Reliable analysis of low-abundance glycans in complex biological matrices.
✅ Comprehensive Analytical Coverage: From molecular weight profiling to structural characterization and quantitative analysis.
✅ State-of-the-Art Mass Spectrometry Platforms: Equipped with Q-TOF, Orbitrap, and MALDI-TOF systems.
✅ High-Throughput and Automated Workflows: Efficiently handle large-scale sample sets, reducing time and cost.
✅ Expert Technical Support: A multidisciplinary team ensures accurate data interpretation and actionable insights.
Sample Submission Suggestions
Mass Spectrometry-Based High-Throughput Glycan Screening Solution is suitable for a wide variety of samples, including blood, urine, tissue extracts, cell culture supernatants, fermentation products, food samples, and purified glycoproteins. Clients are encouraged to provide sample source details and background information to facilitate optimization of the analytical strategy.
MtoZ Biolabs will supply comprehensive submission guidelines, ensuring smooth project execution and reliable data output.
Applications
● Disease Mechanism Studies: Investigating glycan-mediated regulation in cancer, diabetes, and neurodegenerative disorders.
● Biomarker Discovery: Identifying disease-specific glycan structures for early diagnostics and prognosis.
● Biopharmaceutical Quality Control: Evaluating glycosylation consistency and stability in monoclonal antibodies and recombinant proteins.
● Immunology and Pathogen Research: Elucidating pathogen-glycan interactions to guide vaccine development.
● Functional Glycan Studies: Exploring the impact of glycan modifications on protein activity, cell signaling, and metabolic pathways.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: Can mass spectrometry be combined with other separation technologies for improved analysis?
Yes. Mass spectrometry is often integrated with techniques such as HILIC, UHPLC, or CE to enhance separation efficiency and achieve more refined structural resolution. At MtoZ Biolabs, we provide optimized multi-technology integration strategies to deliver comprehensive and reliable glycan profiling results.
MtoZ Biolabs’ Mass Spectrometry-Based High-Throughput Glycan Screening Solution provides fast, precise, and scalable glycan analysis for both academic research and industrial applications. By combining cutting-edge MS platforms with expert bioinformatics, we deliver actionable insights that accelerate glycomics research, biomarker discovery, and biopharmaceutical development.
Contact us today to discuss your project and receive a tailored solution for your glycan research needs.
Related Services
High-Throughput Glycan Screening Solution
Automated LC-MS-Based High-Throughput Glycan Screening Solution
CE-MS-Based High-Throughput Glycan Screening Solution
ESI-MSn-Based High-Throughput Glycan Screening Solution
MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution
HPLC-Based High-Throughput Glycan Screening Solution
UHPLC-FLD-Based High-Throughput Glycan Screening Solution
Hydrophilic Interaction Chromatography-Based High-Throughput Glycan Screening Solution
How to order?
