High-Throughput Glycoengineered Cell Line Glycan Screening Service
- Cell Line Screening and Optimization
- Biopharmaceutical Development
- Glycosylation Research and Glycomics
- Vaccine and Immunotherapy Development
- Drug Target Screening and Drug Discovery
High-Throughput Glycoengineered Cell Line Glycan Screening Service is a high-throughput screening service designed for glycoengineered cell lines (GCLs), aiming to systematically analyze and compare glycans produced by different glycoengineered cell lines using advanced separation and detection technologies. Through this service, clients can obtain detailed glycosylation data that reveal the impact of various glycoengineering strategies on glycan patterns, thereby providing critical support for glycoprotein production and functional optimization.
Glycosylation is one of the most common and complex post-translational modifications of proteins, widely involved in protein folding, secretion, signal transduction, and immune recognition. Distinct glycan structures directly influence glycoprotein stability, pharmacokinetics, and immunogenicity. With the advancement of glycoengineering technologies, cell lines can be modified through gene editing, metabolic regulation, or synthetic biology approaches to specifically produce glycoproteins with defined glycan structures, thereby optimizing the quality and efficacy of therapeutic proteins such as monoclonal antibodies and recombinant enzymes. To rapidly screen and identify glycan features generated by different engineered cell lines, high-throughput glycan analysis platforms have become indispensable tools. Such platforms are not only suitable for screening glycoprotein-producing cell lines but also provide powerful data support for glycosylation mechanism studies, glycobiology research, and the development of related therapeutics.
Narimatsu Y. et al. J Biol Chem. 2021.
Figure 1. Glycoengineering Strategies for Recombinant Glycoprotein Therapeutics.
Services at MtoZ Biolabs
Based on advanced chromatography and mass spectrometry platforms, MtoZ Biolabs provides High-Throughput Glycoengineered Cell Line Glycan Screening Service to rapidly and systematically screen and analyze glycan modification features of glycoengineered cell lines, comprehensively evaluating glycan structural composition, abundance distribution, and functional relevance. This service delivers efficient and reliable data support for glycoprotein drug development, glycomics research, and process optimization. Our offerings include but are not limited to:
UHPLC-FLD-Based High-Throughput Glycan Screening Solution
Utilizing ultra-high-performance liquid chromatography combined with fluorescence detection, this solution enables highly sensitive and high-resolution analysis of glycan structures and chain lengths, making it suitable for rapid screening of large sample sets.
CE-MS-Based High-Throughput Glycan Screening Solution
Through the integration of capillary electrophoresis and mass spectrometry, this solution precisely resolves differences in mass-to-charge ratios and migration properties of glycans, allowing for efficient identification and quantification, especially well-suited for in-depth analysis of complex mixtures.
Service Advantages
Advanced Analysis Platform: MtoZ Biolabs established an advanced High-Throughput Glycoengineered Cell Line Glycan Screening Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all igh-Throughput Glycoengineered Cell Line Glycan Screening data, providing clients with a comprehensive data report.
Flexible Customization Service: Screening strategies can be tailored to customer needs, allowing flexible adaptation to different glycan types, glycoengineered cell lines, and research objectives.
Sample Submission Suggestions
Sample Types
Culture supernatant from glycoengineered cell lines, purified glycoproteins, cell lysates, and other types of samples are accepted.
Storage and Transportation
All samples are recommended to be stored at −80°C to avoid repeated freeze-thaw cycles, and transportation should be carried out on dry ice.
It is recommended to contact the MtoZ Biolabs technical team prior to sample submission to obtain detailed and tailored guidelines for sample preparation and submission.
Applications
Application examples of High-Throughput Glycoengineered Cell Line Glycan Screening Service:
Rapidly compare the glycan profiles of different glycoengineered cell lines to select the production cells that best meet drug quality requirements.
Optimize glycoprotein production processes to improve drug immunogenicity, pharmacokinetic properties, and efficacy.
Screening and analyzing different glycan types helps researchers gain deeper insights into the regulation of protein functions by glycosylation and promotes fundamental studies in glycobiology and glycomics.
Identify the effects of glycans on the immune system to support the design and optimization of glycosylated vaccines and improve immune response efficacy.
Facilitate the identification of new glycan targets and promote the development of innovative drugs.
FAQ
Q1: What Types of Glycans Can This Service Analyze?
A1: MtoZ Biolabs can characterize N-glycans, O-glycans, and complex mixed glycans, and perform quantitative and structural analysis of modification features such as fucosylation, sialylation, and galactosylation.
Q2: Can Screening Still Be Performed if the Sample Amount Is Limited?
A2: Yes, MtoZ Biolabs’ highly sensitive UHPLC-FLD and CE-MS platforms can achieve reliable detection with glycoprotein samples as low as the microgram level, making them especially suitable for precious samples in clinical and research applications.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Glycobiology Microarray Service
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