Resources

Proteomics Databases



Metabolomics Databases

• • The Ultimate Guide to Mass Spectrometry in Proteomics: Maximize Data Quality

  Mass spectrometry in proteomics is crucial in modern biomedical research and is widely used in protein identification, quantitative analysis, functional studies, and disease diagnosis. By leveraging mass spectrometry, researchers can precisely elucidate protein structures and functions, advancing biological research. However, the vast and intricate nature of mass spectrometry datasets poses significant challenges for accurate and efficient interpretation. This guide aims to equip researchers with effe......

• • Proteomics Analysis: 12 Key Mistakes to Avoid for Accurate Results

  Proteomics analysis involves the identification, quantification, and characterization of post-translational modifications in proteins. Despite substantial advancements in high-resolution mass spectrometry and bioinformatics tools, mistakes in experimental workflows remain a critical factor affecting data reliability. This review highlights 12 common pitfalls in proteomics analysis and presents corresponding strategies to improve experimental accuracy and reproducibility.   Mistakes in the Sample Prepa......

• • 7 Proven Ways to Enhance Protein Identification Accuracy

  Protein identification is a key step in proteomics research, determining the accuracy and reliability of analysis results. Accurate protein identification not only helps deepen the understanding of biological processes but also provides scientific evidence for disease research and drug development. However, due to factors like sample complexity, sample loss during processing, and the precision of mass spectrometry techniques, identification results may have uncertainties. Therefore, laboratories often......

• • How to Optimize Proteomics Workflows for Faster and More Accurate Results

  Proteomics analysis is a tool for studying the composition, expression levels, interactions, and dynamic changes of all proteins within biological systems. With the deepening of life science research, its applications in disease diagnosis, drug development, and personalized medicine are increasingly widespread. However, due to the complexity of proteins and the challenges in data analysis, optimizing workflows to enhance sensitivity, specificity, and speed in obtaining accurate results has become a pr......

• • Applications and Current Status of Native Mass Spectrometry in Biomolecule Research

  Native mass spectrometry analysis, a powerful technique for directly analyzing intact biomolecules, has found extensive applications in structural biology, proteomics, interaction network analysis, and biopharmaceutical research. Unlike traditional enzymatic digestion-based mass spectrometry, this method enables the direct detection of biomolecules in their native states, including proteins, protein complexes, nucleic acids, and their interactions. Native mass spectrometry analysis offers unique insig......

• • Proteomics: Key Steps, Tools, and Best Practices

  Proteomics is the scientific field that studies the composition, structure, function, and interactions of proteins within living organisms. By comprehensively analyzing the proteome, researchers can gain in-depth insights into the dynamic changes in biological systems, providing crucial information for disease research, drug development, and biomarker discovery. Proteomics not only helps understand the end products of gene expression but also reveals disease mechanisms, advancing the development of.........

• • Membrane Protein Analysis

  Membrane protein analysis is a specialized area within proteomics that focuses on the investigation of proteins embedded in or associated with the cell membrane. These proteins are integral to essential biological functions, serving as receptors in signaling pathways, ion channels, transporters, and components of intercellular junctions. Given the structural complexity and dynamic nature of membrane proteins, comprehensive analysis poses significant challenges. Such analyses are crucial not only for e......

• • MALDI-TOF Analysis of Protein

  MALDI-TOF analysis of protein, also known as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), is a key analytical technique in modern proteomics research. By employing a unique mass spectrometry approach, this technique enables accurate molecular weight determination of proteins and peptides. MALDI-TOF analysis of protein utilizes matrix-assisted laser desorption/ionization (MALDI) to ionize protein molecules in a sample, followed by time-of-flight (TOF) mas......

• • Mass Spectrometry Bacterial Identification

  Mass spectrometry bacterial identification is a rapid and accurate method for microbial identification leveraging mass spectrometry technology. By analyzing the protein profile of bacteria, this approach allows for the identification of bacterial species in a remarkably short time. Compared to traditional culture and biochemical testing methods, it offers superior efficiency and precision. This technique finds applications across clinical microbiology, food safety, environmental monitoring, and biopha......

• • Label-Free Shotgun Proteomics

  Label-free shotgun proteomics represents an advanced technique in proteomics analysis, notable for its independence from labeled compounds in sample quantitation. Unlike traditional labeling strategies, such as iTRAQ or SILAC, this method uses the raw signals obtained by a mass spectrometer for relative quantification. The fundamental approach involves directly digesting complex biological samples to produce peptide mixtures, which are then analyzed by high-resolution mass spectrometry to identify and......