Resources

Proteomics Databases



Metabolomics Databases

• • Type IV Collagen Test

  Significance of Type IV Collagen Detection: Type IV collagen is a crucial component of the basement membrane, serving as a primary structural element in numerous organs and tissues. It plays an essential role in various physiological processes such as cell migration, growth, differentiation, apoptosis, and disease progression. Consequently, Type IV collagen detection is extensively utilized in clinical diagnostics and disease research.

• • Bradford Protein Content Determination Experiment Report

  Objective: The aim of this experiment is to quantify protein concentration via the Bradford assay. Principle: The Bradford assay is based on the binding of Coomassie Brilliant Blue G-250 to proteins, forming a stable blue complex. The absorbance of this complex peaks at 595 nm when in an acidic environment, enabling the determination of protein concentration.

• • Numerical Y-Coordinate of Circular Dichroism Spectrum

  In circular dichroism (CD) spectroscopy, the vertical axis represents either the absorbance or transmittance of light, determined by the ratio of the intensity of light passing through the sample to that passing through a reference material. This ratio is commonly referred to as "transmittance," denoted by "T," or "absorbance," denoted by "A," with absorbance often being the logarithmic form of the transmittance ratio. Circular dichroism is an analytical technique that assesses the chemical composition a...

• • Host Residual DNA Detection

  In the fields of biological research and bioproduct manufacturing, particularly during biopharmaceutical production, the detection of host residual DNA represents an indispensable quality control measure. Residual DNA from host organisms may persist through production processes, potentially compromising the quality, safety, and therapeutic efficacy of the final product.

• • Sample Loading Requirements for Circular Dichroism Spectroscopy Protein

  Circular dichroism (CD) spectroscopy is a technique used to analyze protein structures based on their interaction with circularly polarized light, enabling the study of protein folding and conformational changes. Accurate sample preparation is crucial for obtaining high-quality results in such analyses.

• • Unlocking the Molecular Code: Applications of Protein Interaction Analysis in Research and Therapeutics

  MtoZ Biolabs offers state-of-the-art protein interaction analysis services by integrating CST pull-down assay kits with our advanced UPLC-MS platform. This comprehensive approach enhances both the precision and efficiency of protein interaction studies, enabling the accurate identification of complex protein samples and supporting high-throughput screening and rapid detection. Our UPLC-MS system provides precise mass and structural analysis of proteins isolated from pull-down assays, delivering detailed....

• • Single-Cell Spatial Transcriptomics

  Single-cell spatial transcriptomics is an innovative technology that integrates single-cell transcriptomics with spatial information, allowing for the precise measurement of gene expression in individual cells within tissue sections. By achieving single-cell resolution, this method has transformed biological and medical research. Unlike traditional transcriptomics, which averages gene expression data across large populations of cells and overlooks cellular heterogeneity, single-cell spatial transcript......

• • Cryo-EM

  Cryo-electron microscopy (Cryo-EM) is a transformative imaging technique for investigating the three-dimensional structures of biological macromolecules. By operating at cryogenic temperatures, Cryo-EM allows scientists to capture detailed images of proteins, viruses, and other complex biomolecules in their native states. The method involves rapidly freezing samples to vitrify them in amorphous ice at liquid nitrogen temperatures, preserving their natural structure and preventing distortions caused by......

• • Isolation, Purification, and Characterization of Enzymes

  The isolation, purification, and characterization of enzymes are critical processes in modern biochemistry and biotechnology. These methods enable researchers to extract specific enzymes from complex biological samples and thoroughly analyze their properties. Enzymes, as essential biological catalysts, drive nearly all biochemical reactions, playing pivotal roles in metabolism, signal transduction, cellular communication, and gene expression. Studying the structure, function, and roles of enzymes in b......

• • Protein Sequencing Using Mass Spectrometry

  Protein sequencing using mass spectrometry is a cutting-edge technology for determining the amino acid sequence of proteins with precision. By leveraging mass spectrometry, researchers can obtain detailed information on the amino acid composition and sequence of proteins. The core principle involves ionizing protein samples, fragmenting them into smaller peptide chains, and measuring their mass-to-charge ratio (m/z) to deduce the amino acid sequence. This approach is central to proteomics, offering va......