Organelle Isolation and Protein Purification Service

Subcellular structures such as mitochondria, endoplasmic reticulum, Golgi apparatus, chloroplasts, and nuclei play vital roles in maintaining cellular homeostasis, signal transduction, energy metabolism, and disease progression. Accurate isolation of these organelles and subsequent protein purification is fundamental for studying organelle-specific functions, protein interactions, signaling regulation, and the discovery of therapeutic targets.

 

However, subcellular fractionation poses significant challenges due to complex structures, cross-contamination, and protein instability, requiring advanced platforms and highly skilled personnel. To meet the demands of high-level research and pharmaceutical development, MtoZ Biolabs offers a specialized Organelle Isolation and Protein Purification Service, integrating efficient separation techniques with advanced mass spectrometry platforms to provide standardized, traceable solutions for subcellular proteomics.

 

Services at MtoZ Biolabs

Our Organelle Isolation and Protein Purification Service includes the following core modules:

 



Cell Wall Isolation and Cell Wall Protein Extraction Service

The Cell Wall Isolation and Cell Wall Protein Extraction Service at MtoZ Biolabs enables comprehensive proteomic analysis of plant cell wall proteins through high-purity wall isolation and sequential protein extraction, supporting studies on plant development regulation, stress response mechanisms, and functional gene discovery.

 



Membrane and Membrane Protein Extraction Service

This service is designed to assist researchers in efficiently extracting and purifying membrane proteins from complex biological samples using a specialized technical platform, thereby providing high-quality membrane protein samples for downstream biological studies.

 



Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service

This service supports ER isolation from diverse sample types and can be further extended to include protein concentration, quantification, and mass spectrometry analysis, enabling clients to systematically investigate ER functions, identify potential target proteins, and advance disease mechanism research and drug discovery.

 



Mitochondrial Isolation and Mitochondrial Protein Purification Service

MtoZ Biolabs offers a specialized mitochondrial isolation and mitochondrial protein purification service focused on the high-purity separation and extraction of mitochondria and their proteins. This service is applicable to various types of cell or tissue samples. Through optimized isolation workflows and protein extraction strategies, it ensures the recovery of structurally intact and functionally active mitochondrial proteins.

 



Chloroplast Isolation and Chloroplast Protein Purification Service

MtoZ Biolabs offers a robust Chloroplast Isolation and Chloroplast Protein Purification Service built on optimized plant tissue workflows and efficient density gradient protocols. Our service supports a variety of research applications, including photosynthetic regulation, signal transduction, membrane proteomics, and subcellular omics.

 



Nucleus Isolation and Nuclear Protein Purification Service

Our Nucleus Isolation and Nuclear Protein Purification Service delivers high-quality, ready-to-use nuclear extracts from a wide range of biological sources, including mammalian tissues, cultured cells, yeast, and plant cells.

 



Peroxisome Isolation and Purification Service

MtoZ Biolabs offers specialized Peroxisome Isolation and Purification Service that enables researchers to efficiently isolate high-purity peroxisomes from a range of biological sources, including animal tissues, plant cells, and fungi. Our workflows integrate optimized organelle enrichment strategies with downstream compatibility for proteomic and metabolomic profiling, ensuring a solid foundation for functional characterization and disease model development.

 



Golgi Apparatus and Golgi Protein Extraction and Purification Service

The Golgi Apparatus and Golgi Protein Extraction and Purification Service is designed to enable precise isolation of the Golgi apparatus and efficient extraction of its associated proteome, including both membrane-bound and luminal proteins. This service supports a broad range of cell types and tissues.

 



Centrosome Isolation and Centrosome Protein Purification Service

This service enables efficient isolation and purification of centrosomes and their associated proteins from cells or tissues, supporting downstream studies on cell division, signal regulation, and disease mechanisms through high-quality proteomic analysis.

 



Exosome Separation & Purification Service

MtoZ Biolabs’ Exosome Separation & Purification Service integrates advanced methodologies to provide tailored solutions for isolating intact exosomes from complex biological samples (e.g., blood, cell culture supernatant, urine). Through differentiated separation strategies (combining traditional and emerging techniques) and stringent quality control, our service ensures optimized exosome yield, purity, and bioactivity, laying a reliable foundation for downstream functional studies, drug development, and clinical translation.  

 

Exosomal Protein Isolation and Profiling Service

MtoZ Biolabs offers Exosomal Protein Isolation and Profiling Service to efficiently isolate and analyze proteins from exosomes, providing support for understanding the biological functions of exosomes and their potential clinical applications.

 

Service Advantages

1. High-Purity Fractionation

We utilize standardized protocols combined with gradient centrifugation and magnetic bead enrichment to achieve high-purity and reproducibility in subcellular separation while preserving structural integrity. Protocols are tailored for various cell types to minimize cross-contamination. Protease inhibitors, low-temperature procedures, and optimized buffers help preserve native protein conformation and biological activity, supporting downstream functional or structural analyses.

 

2. Versatile Purification Strategies

Our platform integrates multiple purification methods including affinity chromatography, ion-exchange, and gel filtration to isolate and concentrate soluble, membrane-bound, or low-abundance proteins, adapting to various scale and purity requirements.

 

3. Broad Sample Compatibility

MtoZ Biolabs’ Organelle Isolation and Protein Purification Service supports a wide range of sample types, including cell lines, tissues, and clinical specimens, enabling flexible application across diverse experimental needs.

 

4. Integrated One-Stop Workflow

Leveraging our comprehensive proteomics platform, we offer seamless workflows covering organelle isolation, protein purification, MS acquisition, and data analysis—reducing transfer-related errors and enhancing data consistency.

 

Applications

1. Mitochondrial Function Studies: Isolate mitochondria for analysis of oxidative phosphorylation-related proteins.

2. Signal Transduction Research: Extract nuclear or membrane proteins for transcription factor or receptor pathway studies.

3. Membrane Protein Target Discovery: Isolate membrane fractions for antibody screening or structural analysis.

4. Stress and Apoptosis Mechanisms: Investigate ER stress or lysosomal rupture in subcellular stress responses.

5. Subcellular Proteomics: Establish proteome profiles of organelles using LC-MS/MS.

 

FAQs

Q1: Can the isolated organelles be used for Western blotting?

A: Yes. We provide protein quantification results and buffer recommendations for immediate downstream applications like Western blotting.

 

Q2: Are the isolated organelles suitable for functional studies?

A: Under appropriate conditions, the isolated structures retain functional integrity and can be used for assays such as respiratory chain activity or enzymatic function testing.

 

Sample Submission Suggestions

1. Sample Types: Cell pellets or fresh/frozen tissues

2. Recommended Quantity: ≥1 × 10⁷ cells or ≥100 mg tissue

3. Storage: Rapid freezing in liquid nitrogen is recommended; ship on dry ice

4. Notes: Avoid repeated freeze-thaw cycles; include a sample information sheet with source, handling, and target organelle details.

 

Deliverables

1. Isolated organelle fractions or protein extracts

2. Protein quantification and purity reports (including SDS-PAGE and optional WB images)

3. Detailed isolation and extraction protocols

4. Optional MS-based proteomics results and raw data files

5. Technical service report documenting procedures and recommendations

 

MtoZ Biolabs combines efficient workflows and advanced mass spectrometry platforms to provide reliable Organelle Isolation and Protein Purification Service for global researchers and biotech customers. Reach out to MtoZ Biolabs to experience expert support for your research with precision and excellence.