Golgi Apparatus and Golgi Protein Extraction and Purification Service
The Golgi Apparatus and Golgi Protein Extraction and Purification Service is designed to enable precise isolation of the Golgi apparatus and efficient extraction of its associated proteome, including both membrane-bound and luminal proteins. This service supports a broad range of cell types and tissues. As a central organelle responsible for protein processing, glycosylation, and vesicle trafficking, the Golgi apparatus plays a pivotal role in secretion, intracellular signaling, and disease progression. However, due to its complex membranous structure, achieving both high purity and structural integrity during extraction is technically challenging. Therefore, a systematic and reproducible isolation protocol is essential to provide high-quality input for downstream proteomic, post-translational modification (PTM), and protein–protein interaction studies.
Leveraging extensive experience in organelle biology and an in-depth understanding of Golgi structure, MtoZ Biolabs has established a robust and scalable platform for Golgi fractionation and protein extraction, compatible with diverse biological specimens. Supported by state-of-the-art high-resolution mass spectrometry systems—including Thermo Fisher Q Exactive HF, Orbitrap Fusion, Orbitrap Fusion Lumos, and Agilent Q-TOF—we deliver high-purity extracts for reliable proteomic investigation, facilitating confident and efficient data acquisition for research workflows.
Services at MtoZ Biolabs
To support diverse research needs, MtoZ Biolabs provides a comprehensive suite of standardized and customized services for Golgi apparatus isolation and protein extraction, ensuring efficient separation and high-quality recovery from a wide range of sample types
· Isolation and purification of single Golgi organelles (applicable to cell lines, animal tissues, and plant specimens)
· Multi-organelle isolation and enrichment (ideal for comparative studies involving multiple compartments)
· Golgi-specific protein extraction (targeting both membrane and luminal components)
· Downstream proteomics-ready processing (supporting PTM mapping, interactome profiling, and quantitative proteomics)
Analysis Workflow
Why Choose MtoZ Biolabs?
✔ High-Purity Organelle Fractionation Platform: Employs advanced centrifugation and density-based separation to yield highly enriched Golgi fractions.
✔ Native-State Protein Extraction Strategies: Non-denaturing protocols preserve protein conformation and biological activity for functional studies.
✔ Extensive Experience with Diverse Sample Types: Supports a wide range of biological matrices including mammalian cells, rodent tissues, and plant material.
✔ Comprehensive Quality Control System: Combines marker-based validation (e.g., Golgin-97), total protein quantification, and visualization techniques to ensure reproducibility.
✔ Broad Downstream Compatibility: Extracted proteins are compatible with LC-MS/MS, glycoproteomics, immunoassays, and structural biology applications.
Applications
· Functional Characterization of Golgi Proteins: Investigate the roles of membrane and luminal Golgi proteins in vesicle trafficking, glycosylation, and protein maturation.
· Golgi Protein Alterations in Disease Models: Enables differential expression and PTM profiling of Golgi proteins between normal and disease states, supporting research in cancer, neurodegeneration, and immune disorders.
· Sample Preparation for Membrane and Glycoproteomic Analyses: Provides highly purified Golgi-derived samples suitable for downstream LC-MS/MS, HILIC-LC, and lectin enrichment assays.
· Antibody Development and Immunodetection: Supplies Golgi-enriched antigens for antibody generation, screening, and the development of Golgi-specific immunoassays.
What Could be Included in the Report?
1. Frozen aliquots of isolated Golgi fractions or extracted proteins
2. Detailed protocol documentation and reagent list
3. Validation report including purity assessment (e.g., WB, immunofluorescence)
4. Quantitative data report on protein concentration, volume, and yield
5. Optional: Seamless integration into MtoZ Biolabs’ proteomics pipeline (quantitative profiling, PTM analysis, interactome mapping, etc.)
MtoZ Biolabs’ Golgi Apparatus and Golgi Protein Extraction and Purification Service delivers high-purity, well-characterized research materials essential for investigating organelle biology, protein regulation, and disease mechanisms. In addition to Golgi-focused solutions, we also offer isolation and protein extraction services for mitochondria, endoplasmic reticulum, lysosomes, peroxisomes, and more—laying the foundation for comprehensive organelle-based systems biology and precision mechanistic studies.
Related Services
Golgi Apparatus Analysis Services
Golgi Apparatus Proteomics Services
How to order?
