Golgi Apparatus Proteomics Services
The Golgi apparatus is a critical membrane-bound organelle in eukaryotic cells, functioning as a central hub for protein post-translational modification, sorting, and trafficking. It plays a vital role in glycosylation, phosphorylation, sulfation, and other post-translational modifications (PTMs). Structurally, it consists of stacks of flattened membrane cisternae, whose morphology and molecular composition vary depending on the cell type, developmental stage, and environmental cues.
Dysfunction of the Golgi apparatus is closely linked to a range of pathological conditions, including congenital disorders of glycosylation (CDGs), neurodegenerative diseases, malignant transformation, and viral infections. Alterations in Golgi structure, transport efficiency, and glycosylation patterns are frequently observed as molecular indicators under these conditions. Therefore, comprehensive analysis of the Golgi proteome—including protein composition, PTM status, and dynamic changes—is essential for uncovering the molecular basis of cellular homeostasis, disease mechanisms, and identifying therapeutic targets.
MtoZ Biolabs offers specialized Golgi Apparatus Proteomics Services by integrating advanced mass spectrometry platforms—such as Thermo Fisher Q Exactive HF, Orbitrap Fusion series, Orbitrap Fusion Lumos, and Agilent Q-TOF—with optimized Golgi isolation techniques and robust proteomic workflows. We empower researchers to gain deep insights into organelle biology and regulatory networks associated with disease.
Services at MtoZ Biolabs
To address the structural complexity and functional diversity of the Golgi apparatus, MtoZ Biolabs offers a comprehensive Golgi Apparatus Proteomics Solution that spans sample preparation through in-depth data analysis. Our key service modules include:
· Golgi Protein Composition Profiling
By enriching Golgi fractions and applying high-resolution LC-MS/MS, we identify core structural proteins, trafficking regulators, and functional enzymes. This helps define Golgi-specific proteomic landscapes and compartmental organization.
· Post-Translational Modification (PTM) Analysis
We perform in-depth analysis of PTMs such as glycosylation, phosphorylation, and ubiquitination using enrichment-based methods and PTM-aware MS pipelines. This reveals functional modulation of Golgi-associated proteins under physiological and pathological conditions.
· Quantitative Golgi Apparatus Proteomics
We apply label-free, TMT, or DIA-based quantitation strategies to compare Golgi protein abundance across experimental conditions. These data enable discovery of differentially expressed proteins and pathway activation profiles.
· Protein–Protein Interaction (PPI) Analysis
Using affinity-based enrichment, cross-linking MS, or co-IP coupled with MS, we map interaction networks within the Golgi apparatus, including Golgi-resident enzymes and vesicle trafficking complexes.
Analysis Workflow
Our optimized Golgi apparatus proteomics workflow includes the following steps:
1. Sample Collection & Pretreatment
Extraction of Golgi-rich cellular fractions or tissues using differential centrifugation or commercial Golgi isolation kits.
2. Purity Validation
Verification of organelle enrichment using marker proteins such as Golgin-97 and GM130 by Western blotting or IF.
3. Protein Extraction & Enzymatic Digestion
Solubilization of membrane proteins followed by high-efficiency tryptic digestion to generate peptides suitable for MS analysis.
4. PTM Enrichment (Optional)
Application of affinity-based enrichment for glycopeptides, phosphopeptides, or ubiquitinated peptides, depending on study needs.
5. Mass Spectrometry Analysis
High-resolution MS analysis using Orbitrap or Q-TOF platforms; supports DDA, DIA, and targeted acquisition modes.
6. Bioinformatics & Data Interpretation
Advanced database searching, PTM localization, pathway enrichment, and interaction network analysis using dedicated software tools.
Why Choose MtoZ Biolabs?
✔ Flexible Platform Combinations: Compatibility with multiple MS platforms (Orbitrap, Q-TOF) and acquisition strategies (DDA, DIA, PRM) for different research objectives.
✔ High Sensitivity and Coverage: Use of nano-LC, multi-enzyme digestion strategies, and low-input workflows enables deep proteome coverage from limited starting material.
✔ Comprehensive PTM Profiling: Integrated enrichment and MS analysis support simultaneous identification and quantification of multiple PTMs.
✔ Powerful Bioinformatics Integration: Downstream analysis includes functional annotation, spatial mapping, and dynamic modeling to support biological discovery.
✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
· Organelle Function Studies: Characterization of Golgi-dependent protein sorting, vesicle trafficking, and membrane dynamics.
· Pathological Mechanism Research: Investigation of Golgi structural disruption and glycosylation defects in CDGs, neurodegeneration, and cancer.
· Therapeutic Target Identification: Discovery of drug-regulated proteins and pathways associated with Golgi-mediated trafficking or stress response.
· Subcellular Proteomics Integration: Combined analysis of Golgi, ER, and lysosomal compartments to model vesicle transport networks and protein flow.
FAQ
Q1: Does the hydrophobicity of Golgi membrane proteins hinder proteomics analysis?
Indeed, the low solubility and high hydrophobicity of membrane proteins pose analytical challenges. At MtoZ Biolabs, we employ refined extraction and digestion protocols using tailored buffer systems and surfactants to enhance recovery and digestion efficiency without compromising protein integrity. Coupled with our high-sensitivity Orbitrap platforms and experienced team, we achieve robust identification and PTM profiling of Golgi membrane proteins.
Q2: Can targeted analysis be performed for specific proteins or pathways?
Yes. MtoZ Biolabs provides customizable proteomics solutions tailored to specific protein families (e.g., glycosyltransferases, SNAREs) or signaling pathways (e.g., Golgi stress pathway). We incorporate immunoenrichment and targeted MS techniques (PRM/MRM) to enhance specificity and sensitivity. Our team also supports bioinformatics-driven target selection and experimental design to accommodate diverse research goals.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Beyond Golgi Apparatus Proteomics Services, MtoZ Biolabs has established a comprehensive multi-omics platform encompassing various organelles—including mitochondria, peroxisomes, endoplasmic reticulum, and lysosomes. Through platform integration and tailored strategy design, we offer powerful analytical support for organelle biology, disease mechanism exploration, and novel therapeutic target discovery, helping you drive breakthroughs in systems-level research.
Related Services
Golgi Apparatus Analysis Services
Golgi Apparatus and Golgi Protein Extraction and Purification Service
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