Membrane and Membrane Protein Extraction Service
Membrane and Membrane Protein Extraction Service is designed to assist researchers in efficiently extracting and purifying membrane proteins from complex biological samples using a specialized technical platform, thereby providing high-quality membrane protein samples for downstream biological studies.
The cell membrane is a fundamental component of both eukaryotic and prokaryotic cells, forming a physical barrier between the cell and its external environment. Its primary functions include selective permeability, signal transduction, intercellular interactions, and maintaining cell morphology and stability. The cell membrane is primarily composed of phospholipids, cholesterol, and membrane proteins. Among these, membrane proteins play essential roles in cellular communication, molecular transport, ion homeostasis, and signal transmission.
Ghazikhani H. Butler G. J Integr Bioinformat. 2023.
Membrane proteins can be classified into two main types: integral membrane proteins and peripheral membrane proteins. Integral membrane proteins span the lipid bilayer and are involved in transmembrane transport and signal transduction, while peripheral membrane proteins interact non-covalently with the membrane surface and are often associated with signal transduction and cytoskeletal connections. Studying membrane proteins is crucial for understanding cellular physiological processes, disease mechanisms, and drug development. The extraction and purification of membrane proteins serve as the foundation for functional studies, structural analysis, and drug screening. Using detergents and non-polar solvents, membrane proteins can be effectively isolated from cell membranes while retaining their native conformation and biological function.
Leveraging advanced membrane protein extraction techniques and multiple purification strategies, MtoZ Biolabs offers Membrane and Membrane Protein Extraction Service to efficiently extract and purify membrane proteins from complex samples, and provide high-quality membrane protein samples for subsequent functional analysis, structural research and drug screening. Additionally, we offer downstream analysis services related to membrane protein modifications, structural characterization, and interaction profiling.
Analysis Workflow
The main workflow of Membrane and Membrane Protein Extraction Service is as follows:
1. Sample Preparation
Crude membrane protein extracts are obtained from tissues or cells through mechanical disruption or lysis.
2. Membrane Protein Extraction
Appropriate detergents or solvents are used to extract membrane proteins from the cellular membrane.
3. Membrane Protein Separation
Integral and peripheral membrane proteins are separated using methods such as density gradient centrifugation, affinity chromatography, or ion exchange chromatography.
4. Purification and Concentration
Membrane proteins are further purified using chromatographic techniques to obtain high-purity samples. Concentration can be performed as needed for downstream analysis.
5. Quality Control and Validation
The purity and extraction efficiency of membrane proteins are verified using SDS-PAGE, electrophoretic mobility assays, Western blotting, and other analytical methods.
Service Advantages
1. High Extraction Efficiency: Utilizes optimized extraction techniques to efficiently isolate membrane proteins from complex samples, ensuring high recovery rates.
2. High Purity: Employs multiple separation and purification strategies to deliver membrane protein samples with high purity, suitable for downstream functional and structural analyses.
3. Native Conformation Preservation: Uses mild detergents and solvents to maintain the native structure and functional integrity of membrane proteins.
4. Diverse Separation Techniques: Integrates methods such as centrifugation and chromatography to accommodate various membrane protein extraction needs.
5. Customized Services: Offers personalized services based on customer requirements to meet different research objectives and experimental demands.
Sample Submission Suggestions
Sample Type: Fresh or cryopreserved cells, tissue samples, or subcellular fractions enriched in membrane structures are recommended.
Storage Conditions: Samples should be stored at -80°C and avoid repeated freeze-thaw cycles. For liquid samples, it is recommended to add protease inhibitors.
Shipping Method: Ship on dry ice to maintain low temperature throughout transport and prevent protein degradation.
Additional Notes: If specific buffer systems, detergents, or separation strategies are required, please consult with our technical team prior to submission.
Applications
Application examples of Membrane and Membrane Protein Extraction Service:
Cell Signal Transduction Studies
Extracted membrane proteins can be used to investigate how cells respond to external signals such as hormones and neurotransmitters.
Membrane Protein Functional Analysis
Facilitates the study of the roles membrane proteins play in various biological processes.
Drug Development and Screening
Provides high-quality research materials for drug development targeting membrane protein-related pathways.
Disease Mechanism Research
Helps uncover the molecular mechanisms by which membrane proteins contribute to the development of various diseases.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. High-purity membrane protein samples
4. Quality control report
5. Optional quantitative and qualitative analysis report
Related Services
Membrane Protein Identification Service
Membrane Proteins Structure Characterization Service | Cryo-EM
How to order?
