Membrane Proteomics Services
- Density Gradient Centrifugation: Separates cellular components based on buoyant density, allowing efficient recovery of plasma or organellar membranes.
- Immunoaffinity Magnetic Beads: Uses membrane-specific antibodies conjugated to magnetic beads to selectively isolate targeted membrane populations.
- Biotin Labeling of Surface Proteins: Labels extracellular domains with biotin for subsequent capture and purification, enabling selective enrichment of surface membrane proteins.
- Membrane Protein Extraction: We use optimized protocols involving organic solvent solubilization with detergents to effectively extract both integral and peripheral membrane proteins while preserving protein structure and yield.
- Membrane Protein Separation and Identification: Extracted proteins or peptides are separated by gel electrophoresis or liquid chromatography to reduce complexity. We offer both bottom-up (peptide-based) and top-down (intact protein-based) MS strategies. Bottom-up proteomics is ideal for identifying hydrophobic membrane proteins, while top-down proteomics enables precise mapping of post-translational modifications.
- We offer both label-based and label-free quantification methods. Label-based quantification methods include SILAC, TMT, and iTRAQ. For label-free quantification, we provide SRM/MRM, PRM, and SWATH-MS.
- Analysis of membrane protein domains, topologies, and post-translational modifications to support functional and mechanistic studies.
- Investigation of membrane protein interactions with small molecules, antibodies, ligands, or other biomolecules using techniques such as crosslinking MS, pull-down assays, or affinity capture.
- We offer integrated bioinformatics analysis for membrane proteins, including data output statistics and quality control, subcellular localization and topology prediction, Gene Ontology (GO) and KEGG pathway annotation, protein-protein interaction mapping, and differential expression and enrichment analysis.
Biological membranes are fundamental structures that maintain cellular integrity, compartmentalize intracellular processes, and regulate the exchange of materials between cells and their environment. Composed primarily of lipid bilayers and embedded proteins, membranes are essential for maintaining homeostasis, mediating signal transduction, and supporting energy conversion in both prokaryotic and eukaryotic systems. Among membrane components, membrane proteins play especially critical roles. They serve as receptors, channels, transporters, enzymes, and adhesion molecules, participating in a wide range of physiological processes such as signal reception, nutrient transport, intercellular communication, and immune recognition. Despite representing approximately 30% of the proteome and being the target of over half of all approved drugs, membrane proteins remain one of the most technically challenging classes of proteins to study due to their hydrophobicity, low abundance, and structural complexity.
Figure 1. Functions of Membrane Protein
Membrane proteomics focuses on the large-scale identification, quantification, and characterization of membrane-associated proteins. This field has become increasingly important for understanding disease mechanisms, identifying biomarkers, and discovering novel drug targets. Through high-resolution mass spectrometry and refined enrichment techniques, membrane proteomics enables researchers to elucidate dynamic changes in membrane composition under different physiological and pathological conditions.
Service at MtoZ Biolabs
MtoZ Biolabs offers comprehensive Membrane Proteomics Services to help researchers uncover the complexity of membrane proteins in various biological contexts. Our service combines cutting-edge technologies, including high-resolution mass spectrometry (MS), innovative sample preparation techniques, and specialized bioinformatics analysis, enabling detailed identification, quantification, and characterization of membrane proteins. Our expert team is equipped to handle the unique challenges associated with membrane proteomics, delivering high-quality data that supports both basic research and therapeutic applications.
The core services we provide include:
1. Cell Membrane Enrichment
2. Membrane Protein Extraction, Separation, and Identification
3. Quantitative Analysis of Membrane Proteins
4. Structural Characterization of Membrane Proteins
5. Membrane Protein–Molecule Interaction Analysis
6. Bioinformatics Analysis of Membrane Proteins
In addition to core analytical services, MtoZ Biolabs provides tailored membrane protein solutions to support diverse areas of biomedical research and drug development, including:
💠Membrane Protein Antibody Development Service
💠Membrane Protein-Based Vaccine Development Service
💠Membrane Protein-Based Small Molecule Drug Discovery Service
💠Membrane Protein-Based CAR-T Therapy Development Service
💠Membrane Protein-Based Ligand Discovery Service
💠Membrane Protein-Based Immunoassay Development Service
💠Membrane Protein-Based Cancer Biomarker Discovery Service
Analysis Workflow
Service Advantages
✔️Comprehensive One-Stop Platform
We provide an end-to-end solution covering membrane isolation, protein extraction, identification, quantification, and bioinformatics analysis, streamlining the entire workflow in one service.
✔️Expertise in Challenging Targets
Our team has extensive experience in handling hydrophobic, low-abundance, and multi-span membrane proteins, ensuring high-quality data even for difficult targets.
✔️High-Resolution Mass Spectrometry
Equipped with state-of-the-art instruments, we deliver high sensitivity, resolution, and accuracy in membrane protein identification and PTM analysis.
✔️Specialized Bioinformatics Support
Our integrated analysis pipeline provides in-depth functional annotation and interpretation, helping translate complex proteomics data into meaningful biological insights.
Sample Submission Suggestions
Notes: Avoid using buffers containing SDS, EDTA, or high concentrations of glycerol, as these may inhibit enzymatic digestion or interfere with mass spectrometry. If non-standard buffers are used, please specify their composition in advance.
*Please consult us for tailored sample collection and storage protocols to maximize data quality.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Our Membrane Proteomics Services are designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
Related Services
Membrane Protein Microarray Screening Service
How to order?
