Membrane Protein-Based Cancer Biomarker Discovery Service
- Screening of specific diagnostic biomarkers for different cancer types
- Tumor classification and staging based on membrane protein expression profiles
- Predicting response to targeted therapies and inter-individual variability
- Identification of targets for monoclonal antibody or CAR-T cell therapies
- Comparative analysis of membrane protein expression across disease stages or treatment groups
Membrane Protein-Based Cancer Biomarker Discovery Service leverages membrane protein enrichment, quantitative mass spectrometry, membrane protein microarrays, and bioinformatics mining to systematically identify membrane proteins associated with tumor initiation and progression, while assessing their potential as diagnostic or therapeutic biomarkers.
Membrane proteins are proteins that are tightly associated with cellular or organelle membranes and play central roles in processes such as cell communication, signal transduction, molecular transport, and immune recognition. Due to their direct exposure on the cell surface, membrane proteins are not only accessible to therapeutic targeting but also serve as ideal biomarker sources detectable in bodily fluids.
Cancer development involves gene expression reprogramming, disrupted signaling networks, and altered cellular behavior. Throughout this process, membrane proteins often undergo significant changes in expression levels, post-translational modifications, and subcellular localization. Analyzing these changes provides valuable insights for cancer diagnosis, prognosis evaluation, and the development of targeted therapies.
Lin CY. et al. Nat Commun. 2019.
Figure 1. Membrane protein (MP) communities and cancer membrane protein-regulated networks (CaMPNets) across human cancers.
Based on the advanced mass spectrometry platform, MtoZ Biolabs provides Membrane Protein-Based Cancer Biomarker Discovery Service to help systematically explore membrane proteins that are differentially expressed in different stages or states of tumors, and identify diagnostic, prognostic or therapeutic markers with potential clinical application value. We support a variety of quantitative strategies (such as Label-free, TMT, iTRAQ) for membrane proteomics analysis, and can provide supporting membrane protein microarrays, etc., to provide one-stop research support for the discovery and screening of membrane protein markers.
Analysis Workflow
The general workflow of Membrane Protein-Based Cancer Biomarker Discovery Service is as follows:
1. Membrane Protein Enrichment
Isolate high-purity membrane proteins using techniques such as ultracentrifugation, density gradient centrifugation, or magnetic bead affinity purification.
2. Protein Digestion and Peptide Purification
Digest proteins with trypsin or Lys-C, followed by desalting to prepare samples for mass spectrometry analysis.
3. Quantitative Proteomic Analysis
Perform analysis using high-resolution mass spectrometry platforms.
Quantitative strategies such as Label-Free, TMT, or iTRAQ can be selected based on project needs.
4. Bioinformatics Analysis
Includes protein identification, differential expression analysis, GO/KEGG functional annotation, and pathway enrichment analysis.
5. Functional Validation (Optional)
Validate candidate antigens or ligands using membrane protein microarrays to assess binding activity.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Membrane Protein-Based Cancer Biomarker Discovery Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Membrane Protein-Based Cancer Biomarker Discovery data, providing clients with a comprehensive data report.
4. Flexible and Diverse Strategies: Offers dual approaches for biomarker discovery, combining mass spectrometry-based membrane proteomics and membrane protein microarrays.
5. High Enrichment Depth for Membrane Proteins: Utilizes optimized isolation strategies to minimize cytoplasmic contamination and effectively enrich transmembrane and surface proteins.
Sample Submission Suggestions
Sample Types: Compatible with cells, tissues, and enriched membrane protein samples.
Storage and Shipping: Samples should be stored at −80°C and shipped on dry ice.
Additional Notes: Please contact our technical support team prior to submission for detailed and customized sample preparation guidance.
Applications
Membrane Protein-Based Cancer Biomarker Discovery Service is applicable to a wide range of cancer research scenarios, from basic studies to translational medicine, including but not limited to:
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Membrane and Membrane Protein Extraction Service
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