Membrane Protein Antibody Development Service
Membrane Protein Antibody Development Service refers to a one-stop technical service that takes membrane proteins as targets, combines the structural characteristics of membrane proteins, functional conformation maintenance strategies and diversified antibody discovery platforms, and systematically carries out membrane protein expression and preparation, targeted antibody screening, verification and functional evaluation.
Membrane proteins are widely distributed across cell membranes and subcellular membrane structures, playing a pivotal role in mediating cellular communication with the external environment. G protein-coupled receptors (GPCRs), ion channels, transmembrane enzymes, and transporters are among the key membrane proteins implicated in numerous pathological processes, including inflammation, cancer, autoimmune diseases, and neurological disorders. Due to their surface-exposed topology and modifiable functional sites, membrane proteins represent one of the most prominent target classes for antibody therapeutics—over 40% of approved antibody drugs target membrane proteins. However, challenges such as high hydrophobicity, complex conformations, and poor stability of membrane proteins often hinder their expression, result in loss of native antigenic conformation, and reduce the success rate of specific antibody recognition during discovery. Therefore, presenting antigens in their native conformation and enabling high-throughput, highly specific antibody screening are central to the development of membrane protein-targeted antibodies.
Salazar GT. et al. Engineering. 2021.
Utilizing multidisciplinary knowledge and technologies such as structural biology, membrane protein chemistry and antibody engineering, MtoZ Biolabs provides Membrane Protein Antibody Development Service including membrane protein expression and preparation, high-throughput antibody screening and functional verification, etc., to provide customers with one-stop membrane protein antibody discovery and development solutions.
Analysis Workflow
The general workflow of Membrane Protein Antibody Development Service includes:
1. Project Evaluation and Antigen Design
Antigen design strategies are customized based on the structural characteristics of the target membrane protein, expression approaches, and specific client requirements.
2. Membrane Protein Expression and Preparation
An appropriate expression system and membrane-mimetic environment are selected to optimize purification conditions, ensuring the production of conformationally stable and functionally active membrane protein antigens.
3. Antibody Discovery and Screening
Antibodies are generated through animal immunization, phage display, or synthetic libraries. Positive clones are identified using high-throughput microarray or cell-based screening platforms.
4. Specificity and Affinity Validation
The binding specificity and affinity of antibodies to the membrane protein are validated using techniques such as SPR, ELISA, and flow cytometry.
5. Functional Assessment and Epitope Mapping
Functional antibodies undergo cell-based or in vitro functional assays, with optional analysis of epitope binding sites and conformational effects.
6. Candidate Antibody Recommendation and Delivery
Based on screening results, high-affinity, high-specificity candidate antibodies are recommended for downstream development.
Service Advantages
1. Expertise in Membrane Protein Expression and Reconstitution: Proficient in various membrane protein expression systems and membrane-mimetic strategies to ensure antigens retain native functionality.
2. Multi-Platform Antibody Discovery Capabilities: Combines traditional immunization with display technologies to accommodate diverse antigen types and research objectives.
3. High-Sensitivity Screening and Validation Platforms: Utilizes microarray and SPR-based systems to enable high-throughput and high-specificity antibody screening.
4. Function-Oriented Antibody Development: Focuses not only on binding affinity but also on functional validation to identify biologically active antibodies.
5. Flexible Customization Options: Offers modular or end-to-end services tailored to client-supplied antigens or specific membrane protein targets.
Sample Submission Suggestions
Sample Types: Acceptable submissions include antigen samples, membrane protein sequence information, and expressed samples or cell lines.
Additional Information: Sample submission recommendations may vary by project. Please contact our technical support team prior to submission for tailored guidance. If you have specific epitope requirements, functional goals, or drug development contexts, kindly indicate them clearly during the project initiation phase.
Applications
Application examples of Membrane Protein Antibody Development Service:
Therapeutic Antibody Development
Develop humanized or fully human antibody drugs targeting membrane protein antigens.
Companion Diagnostic Antibody Production
Generate highly specific antibodies for detection and imaging of membrane protein biomarkers.
Vaccine Antigen Validation
Identify protective epitopes on viral membrane proteins or tumor-associated antigens to support vaccine design.
Functional Blocking/Agonist Antibody Screening
Discover functional antibodies that can modulate membrane protein-mediated signaling pathways.
Research-Grade Tool Antibodies
Produce antibodies for membrane protein localization, enrichment, co-immunoprecipitation, and other basic research applications.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Summary of Antibody Screening Results
4. Affinity and Specificity Validation Data
5. Functional Validation Results
6. Antibody Sequence Information and Expression Clones
7. Project Summary Report
Related Services
Membrane and Membrane Protein Extraction Service
Membrane Protein Structure Determination Service
Membrane Protein Microarray Screening Service
Membrane Protein-Molecule Interaction Analysis Service
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