Nucleus Isolation and Nuclear Protein Purification Service
MtoZ Biolabs offers professional Nucleus Isolation and Nuclear Protein Purification Service designed to support advanced research in gene regulation, chromatin biology, and subcellular proteomics. Whether you're studying transcription factors, post-translational modifications, or nuclear-cytoplasmic protein dynamics, our Nucleus Isolation and Nuclear Protein Purification Service delivers high-quality, ready-to-use nuclear extracts from a wide range of biological sources—including mammalian tissues, cultured cells, yeast, and plant cells.
The nucleus is the command center of the eukaryotic cell, housing the genome and orchestrating gene expression. Nuclear proteins are central to chromatin remodeling, signal transduction, and cell fate decisions. Isolating intact nuclei and obtaining highly purified nuclear proteins are crucial steps for understanding intracellular signaling, building regulatory networks, and accelerating discoveries in disease biology and drug development.
Services at MtoZ Biolabs
Our nucleus isolation platform integrates robust protocols with extensive experience to deliver customized solutions tailored to your sample type and research goals. Key services include:
· Isolation of intact nuclei from tissues, cell lines, yeast, and plants
· Clear separation of nuclear and cytoplasmic fractions with validation
· Structural assessment of nuclear integrity (including envelope and pore complexes)
· High-purity nuclear protein extraction and contaminant removal
· Customizable enrichment workflows and purity profiling
Analysis Workflow
1. Gentle Lysis & Homogenization
Mild detergents (e.g., NP-40) are used to rupture plasma membranes while preserving nuclear integrity—adapted to different sample formats including tissues and adherent/suspension cells.
2. Nucleus Isolation Options
Sucrose Gradient Centrifugation: Ideal for proteomics and nuclear membrane studies requiring ultra-high purity.
REAP Method: A 5-minute rapid isolation protocol for high-throughput or routine assays like Western blot and EMSA.
3. Nuclear Protein Extraction
Using hypotonic lysis buffers, we extract nuclear proteins with preserved activity and compatibility for proteomic or biochemical analysis.
4. Purity & Quality Control
We verify sample quality through marker-based Western blot, SDS-PAGE profiling, and microscopy. Fail-safe reprocessing is available upon request.
Figure 1. Nucleus Isolation and Nuclear Protein Purification Workflow
Why Choose MtoZ Biolabs?
MtoZ Biolabs' Nucleus Isolation and Nuclear Protein Purification Service delivers high-purity nuclear fractions and structurally intact proteins, supporting diverse downstream applications with improved accuracy and reproducibility.
✅ Multi-Species Versatility: From mammals to plants and yeast, we accommodate various sample types with tailored buffer systems and isolation methods.
✅ High Structural Integrity: Isolated nuclei retain intact envelopes and pores, ensuring functional preservation of protein content.
✅ Ultrapure Separation: Cytoplasmic contamination is minimized, delivering clean nuclear fractions ideal for sensitive downstream assays.
✅ Ready for Downstream Applications: Compatible with proteomics, ChIP, EMSA, nuclear imaging, and interaction studies.
✅ Flexible Customization: Extraction protocols and reagents are adapted to meet your unique experimental design.
Applications
· Chromatin and Epigenetic Studies: Explore histone modifications and chromatin dynamics
· Transcription Factor Research: Track activation states and nuclear localization patterns
· Cell Cycle & Apoptosis: Monitor nuclear structural changes during division or cell death
· Nuclear Proteomics: Quantify, identify PTMs, and map interaction networks
FAQ
Q1: Which samples are supported?
We support a wide range of sources including mammalian tissues, adherent and suspension cells, yeast, and plant cells. Each protocol is optimized for structural preservation and maximum yield.
Q2: How do you ensure purity and quality?
Nuclear and cytoplasmic markers (e.g., Lamin B and GAPDH) are analyzed via Western blot. Additional quality checks include SDS-PAGE and microscopy to ensure nuclear integrity and minimal contamination.
Q3: Do you provide guidance on sample preparation?
Yes. We offer detailed SOPs for sample handling, buffer preparation, and cold-chain transport. Our technical team is also available for real-time consultation and troubleshooting.
What Could be Included in the Report?
1. Purified nuclei or nuclear proteins (liquid or lyophilized format)
2. Protein concentration report and SDS-PAGE profiles
3. Purity assessment and structural validation
4. Optional proteomic data (if MS service requested)
5. Full experimental documentation and raw data
Looking for reliable nuclear protein extraction with high structural fidelity and broad downstream compatibility?
MtoZ Biolabs' Nucleus Isolation and Nuclear Protein Purification Service offers a streamlined solution for subcellular proteomics. Contact us today to discuss your project and receive a tailored proposal—we’ll help you advance your workflow with precision and confidence.
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