Nucleolar Proteomics Services
The nucleolus is the most prominent subnuclear structure in eukaryotic cells, primarily involved in ribosomal RNA (rRNA) transcription, processing, and ribosomal subunit assembly. Beyond its canonical roles, growing evidence highlights its critical functions in RNA editing, signal recognition particle (SRP) assembly, cellular stress sensing, cell cycle regulation, and aging. As such, nucleolar proteomics provides a powerful approach to dissect the multifaceted functions and dynamic regulation of this essential organelle.
MtoZ Biolabs offers comprehensive Nucleolar Proteomics Services that leverage state-of-the-art mass spectrometry platforms and deep expertise in subcellular proteomics. Our end-to-end solutions encompass nucleolar isolation, structural mapping, protein identification, and multidimensional bioinformatics—empowering both fundamental discovery and translational research.
Services at MtoZ Biolabs
To support mechanistic insights into the nucleolus’s role in ribosome biogenesis, RNA metabolism, and stress responses, MtoZ Biolabs provides a fully integrated Nucleolar Proteomics Services framework. Our solutions are tailored to the specific sample types and research objectives of each client, ensuring high data quality and analytical reproducibility. We offer the following service modules:
· High-Purity Nucleolar Isolation
Efficient nucleolar extraction from animal, plant, or yeast samples using sucrose gradient or rapid REAP protocols, ensuring suitability for mass spectrometry and molecular studies.
· Functional Characterization of Nucleolar Proteins
Annotation of RNA-related processes (e.g., mRNA export, tRNA maturation, RNA modification) and cellular regulations (e.g., cell cycle, stress, and translational control) through enrichment analyses.
· Quantitative Proteomics
Multiplexed quantification via SILAC, TMT, and iTRAQ or label-free approaches including SWATH, PRM, and SRM—adaptable for both targeted and global studies.
· Protein Domain & Bioinformatics Analysis
Includes domain prediction, homology clustering, evolutionary lineage mapping, subcellular localization, and pathway enrichment.
Analysis Workflow
1. Sample Lysis & Nucleolar Purification
Employ sucrose gradients or REAP methods for isolating nucleoli from various biological matrices.
2. Protein Extraction & Quantification
Use optimized detergent protocols and rigorous washes to enhance nucleolar protein yield and integrity.
3. Enzymatic Digestion & Cleanup
Perform standardized tryptic digestion and desalting to preserve PTMs and improve MS sensitivity.
4. Mass Spectrometry Acquisition
Utilize high-resolution Orbitrap or Q-TOF platforms in DDA, DIA, PRM, or SWATH modes to capture deep and quantitative proteomic profiles.
5. Data Analysis & Bioinformatics
Includes protein identification, PTM site mapping, domain annotation, subcellular localization prediction, and network visualization.
Why Choose MtoZ Biolabs?
✅ Optimized Nucleolar Isolation Across Species: Minimize cross-contamination from organelles like mitochondria, and achieve high-purity nucleolar fractions.
✅ Full Quantitative Flexibility: Supports both label-based (SILAC, TMT, iTRAQ) and label-free (SWATH, PRM, SRM) strategies to meet diverse experimental needs.
✅ High-Resolution Mass Spectrometry Support: Advanced MS systems ensure sensitive detection of low-abundance proteins and high-precision quantification.
✅ Comprehensive Annotation Framework: Integrates multiple databases for functional annotation, including domain prediction, expression profiles, and localization data.
✅ Custom Bioinformatics & Reporting: Includes pathway mapping, differential expression, structural modeling, and tailored downstream experimental guidance.
Applications
· Ribosome Biogenesis: Identify key proteins involved in the assembly and regulation of ribosomal subunits.
· Stress & DNA Damage Response: Characterize nucleolar remodeling and protein turnover under environmental or genotoxic stress.
· Cell Cycle & Senescence Regulation: Analyze nucleolar roles in proliferation control and aging-associated phenotypes.
· Viral Infection Mechanisms: Study host–virus interactions mediated through nucleolar hijacking of RNA and protein processing.
· Evolutionary Proteomics: Perform comparative analyses of nucleolar protein evolution across species.
FAQ
Q1: Do you support cross-species differential nucleolar proteomics?
Yes. Our Nucleolar Proteomics Services support a wide range of species including human, mice, plants, and yeast. We provide homology alignment, conserved domain analysis, and phylogenetic tree construction for evolutionary and comparative proteomics.
Q2: Are nucleolar proteins suitable for post-translational modification (PTM) studies?
Definitely. The nucleolus contains numerous regulatory proteins ideal for PTM analysis. We offer enrichment and quantification of phosphorylation, acetylation, methylation, lactylation, and more to uncover dynamic cellular regulation.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Contact MtoZ Biolabs to receive customized Nucleolar Proteomics Services. Let our high-quality data and integrated platform accelerate your understanding of nucleolar function in development, disease, and cellular stress.
Related Services
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