Mitochondrial Isolation and Mitochondrial Protein Purification Service

Mitochondria are double-membrane organelles within cells, primarily responsible for energy production and oxidative metabolism. They are also involved in a variety of physiological processes, including fatty acid β-oxidation, amino acid metabolism, calcium signaling regulation, and apoptosis. The Mitochondrial Isolation and Mitochondrial Protein Purification Service utilizes differential centrifugation and density gradient separation techniques to efficiently extract highly purified mitochondria. This is followed by the use of lysis buffers and appropriate extraction methods (such as sonication or chemical lysis) to obtain structurally intact and functionally active mitochondrial proteins, providing ideal samples for downstream analyses.

 

The mitochondrial isolation and mitochondrial protein purification service is widely applied in research areas such as metabolic diseases, neurodegenerative disorders, cancer metabolic reprogramming, cellular aging, and stress responses. It helps researchers to elucidate the role of mitochondrial functional changes in disease onset and progression and supports mechanism exploration and target discovery.

 

Services at MtoZ Biolabs

Leveraging advanced analytical platforms, MtoZ Biolabs offers a specialized mitochondrial isolation and mitochondrial protein purification service focused on the high-purity separation and extraction of mitochondria and their proteins. This service is applicable to various types of cell or tissue samples. Through optimized isolation workflows and protein extraction strategies, it ensures the recovery of structurally intact and functionally active mitochondrial proteins. The service provides high-quality starting materials for downstream applications such as proteomics, enzyme activity assays, or post-translational modification studies, supporting the exploration of mitochondrial functions and their critical roles in disease mechanisms.

 

Analysis Workflow

1. Sample Preprocessing

Cell or tissue samples are homogenized to release intracellular components, preparing them for mitochondrial isolation.

 

2. Mitochondrial Isolation

Mitochondria are separated and enriched from cell lysates using differential centrifugation and density gradient centrifugation, removing cytosolic and other organelle contaminants.

 

3. Protein Extraction

Specialized buffer systems are used to lyse mitochondrial membranes, enabling the extraction of various mitochondrial proteins such as membrane proteins and matrix proteins.

 

4. Protein Purification and Concentration

Extracted proteins are purified using ultrafiltration, column chromatography, or magnetic bead-based methods to obtain high-quality proteins suitable for downstream analyses.

 



Liao, C P. et al. PLOS ONE, 2018.

Figure 1. Scheme of Mitochondrial Isolation from Yeast Using Magnetic Beads.

 

Sample Submission Suggestions

1. Sample Type

Supports mammalian cells, tissues, and preliminarily extracted mitochondrial crude fractions. Fresh or liquid nitrogen-frozen samples are recommended.

 

2. Sample Preservation and Transport

Samples should be stored at −80°C immediately after processing and transported on dry ice. Repeated freeze–thaw cycles must be avoided to preserve mitochondrial structure and protein integrity.

 

3. Additional Notes

If the sample has undergone partial processing (e.g., homogenization or initial mitochondrial isolation), please clearly indicate the procedures used and buffer compositions to facilitate optimal downstream processing.

 

Service Advantages

1. Efficient Isolation

Utilizing a combined strategy of differential and density gradient centrifugation, this service rapidly isolates structurally intact and functionally active mitochondria to accommodate diverse experimental needs.

 

2. Precise Extraction

Enables the extraction of key mitochondrial components such as membrane proteins, matrix proteins, and transmembrane complexes, meeting the requirements of downstream proteomics and functional analyses.

 

3. Rigorous Quality Control

Mitochondrial purity is verified through marker protein detection via Western blot or enzymatic activity assays to ensure the reliability of downstream data.

 

4. End-to-End Workflow

Offers a comprehensive process from mitochondrial extraction and protein purification to quality assessment, saving time and ensuring result reproducibility and consistency.

 

Applications

1. Mitochondrial Functional Mechanism Research

Mitochondrial isolation and mitochondrial protein purification service can be used to reveal the core roles of mitochondria in biological processes such as energy metabolism, oxidative phosphorylation, and apoptosis regulation, supporting fundamental mechanism studies.

 

2. Analysis of Mitochondrial Abnormalities in Disease Models

Applicable to studies on metabolic diseases, neurodegenerative disorders, and cardiovascular diseases, this service enables the investigation of mitochondrial protein expression and functional alterations under pathological conditions.

 

3. Proteomics and Post-Translational Modification Research

Provides high-quality sample material for quantitative analysis of mitochondrial proteins and research on post-translational modifications such as phosphorylation and acetylation.

 

4. Drug Target Screening and Validation

Mitochondrial isolation and mitochondrial protein purification service supports the evaluation of drug effects on mitochondrial structure and function, facilitating the identification of potential targets and safety assessment.

 

FAQ

Q1: Can Clients Provide only Cells or Tissue Samples?

A1: Yes. We offer a comprehensive mitochondrial isolation and protein extraction workflow. Clients only need to submit qualified cell or tissue samples, and all subsequent procedures will be performed by our team.

 

Q2: Can the Isolated Mitochondria Be Used for Downstream Mass Spectrometry Analysis?

A2: Absolutely. We provide high-purity mitochondrial protein extraction services. The resulting samples are suitable for mass spectrometry, Western blotting, enzymatic activity assays, and various other downstream applications.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files