Exosomes Labeling and Tracking Service
- Biodistribution Studies: Assess tissue-specific accumulation and clearance patterns of exosomes in vivo.
- Cellular Uptake Analysis: Visualize and quantify exosome internalization by recipient cells under various conditions.
- Drug Delivery Evaluation: Track engineered exosomes carrying therapeutic payloads to evaluate targeting efficiency and delivery kinetics.
- Mechanistic Studies: Investigate the dynamics of exosome release, transport, and intercellular communication pathways.
- Diagnostic Validation: Validate the origin and destination of disease-related exosomes as potential biomarkers.
- Isolated Exosomes: ≥200 μg total protein or ≥100 μL in PBS
- Plasma/Serum: ≥2 mL per sample
- Cell Culture Supernatants: ≥5 mL per sample
- Other Body Fluids: Please inquire for specific requirements
Exosomes play critical roles in intercellular communication by transporting proteins, nucleic acids, lipids, and signaling molecules. Their inherent biocompatibility, stability, and ability to cross biological barriers make them highly attractive as both disease biomarkers and drug delivery vehicles. However, realizing the full therapeutic and diagnostic potential of exosomes relies on a thorough understanding of their in vivo distribution, cellular uptake, and organ-specific accumulation. To elucidate how exosomes behave in biological systems, precise labeling and tracking strategies are essential. These labeling and tracking approaches enable researchers to monitor exosome transport pathways, assess targeting specificity, and evaluate in vivo and in vitro functional outcomes.
Service at MtoZ Biolabs
MtoZ Biolabs offers a specialized Exosomes Labeling and Tracking Service that helps researchers visualize and quantify exosome dynamics with high sensitivity and accuracy. By integrating advanced labeling techniques with state-of-the-art imaging and analytical platforms, including fluorescence microscopes, electron microscopes, and flow cytometers, we provide tailored solutions for both in vivo and in vitro exosome studies. Our service enables comprehensive tracking of exosome biogenesis, release from parental cells, uptake by recipient cells, distribution in tissues, and levels in biological fluids.
We offer multiple labeling and tracking strategies optimized for different experimental settings:
💠Fluorescent Labeling: Ideal for both in vitro and in vivo applications. We utilize lipophilic dyes (e.g., PKH26, DiI, DiR), fluorescent nanomaterials, and genetically encoded fluorescent proteins, as well as antibody-based immunofluorescent labeling to visualize exosomes under imaging platforms.
💠Bioluminescence Imaging (BLI): Suitable for both in vivo and ex vivo tracking, this method uses luciferase-tagged exosomes to enable sensitive, real-time, and non-invasive visualization of exosome distribution and tissue accumulation.
💠Magnetic Resonance Imaging (MRI): Designed for in vivo imaging, we label exosomes with superparamagnetic iron oxide nanoparticles (SPIONs) to allow precise anatomical localization through high-resolution MRI.
💠Computed Tomography (CT): Applicable for in vivo use, CT-compatible contrast agents such as gold nanoparticles or iodine-labeled probes are employed to track exosomes in deep tissues with structural clarity.
Figure 1. Strategies for Tracking Extracellular Vesicles In Vivo
Analysis Workflow
1. Sample Preparation and Exosome Isolation
Biological fluids or cell culture supernatants are processed using ultracentrifugation, size exclusion chromatography (SEC), or immunoaffinity-based methods to isolate pure exosomes.
2. Labeling Strategy Selection
Based on the research objective (in vivo vs. in vitro), appropriate labeling techniques are selected—such as fluorescent dyes, luciferase fusion proteins, immunogenic reporters, or fluorescent nanoparticles.
3. Exosome Labeling
Labeling is performed through direct surface tagging or parent cell engineering. Care is taken to preserve exosome integrity and function.
4. Tracking and Imaging
Labeled exosomes are administered to the desired biological system (cell culture or animal model), followed by real-time tracking using fluorescence microscopy, BLI, MRI, or CT.
5. Data Acquisition and Analysis
Quantitative and spatial data on exosome localization, uptake, and biodistribution are collected and analyzed using advanced imaging software and statistical tools.
Service Advantages
☑️Advanced Analysis Platform: MtoZ Biolabs established an advanced Exosomes Labeling and Tracking Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
☑️Customized Solutions: Offers tailored labeling and tracking protocols according to research goals, sample type, and desired resolution or depth of analysis.
☑️Preservation of Exosome Integrity: Optimized labeling methods ensure minimal impact on exosome structure and biological function, maintaining data reliability.
☑️Integrated Downstream Support: Combine tracking data with follow-up proteomic, transcriptomic, or functional assays to correlate exosome fate with biological effects.
Applications
Sample Submission Suggestions
We accept the following sample types:
Samples should be stored at -80°C and shipped on dry ice. Avoid repeated freeze–thaw cycles.
*Please consult us for tailored collection and storage protocols to maximize data quality.
Deliverables
1. Labeled Exosome Samples
Ready-to-use, fluorescently or luminescently labeled exosomes for downstream applications.
2. Experimental Protocol Summary
Documentation of labeling and tracking procedures, including reagents, conditions, and instrumentation.
3. Imaging Data
High-resolution images files from fluorescence microscopy, BLI, or other platforms.
4. Customized Report
Comprehensive analysis report including tracking results, quantitative assessments, and method validation tailored to the project objectives.
To learn more about our Exosomes Labeling and Tracking Service, or to discuss your specific requirements, please contact us.
Related Services
Exosome Isolation and Purification Service
Exosome Marker Analysis Service
Exosomes Identification Service
Exosomes Metabolomics Services
Exosomal Whole Transcriptome Sequencing Service
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