Resources

Proteomics Databases



Metabolomics Databases

• • The Effects of Different Proteomics Sequencing Methods on Research Outcomes

  Proteomics sequencing, as a core technique in proteomics research, provides abundant information to reveal the complete profile of proteins in biological systems. However, the choice of different methods in proteomic sequencing can significantly impact research results.

• • Protein Secondary Structure Analysis and Circular Dichroism

  Circular dichroism spectroscopy is commonly used to analyze and interpret the secondary structure of proteins. Through circular dichroism, we can obtain valuable information on protein secondary structure and gain a deeper understanding of the relationship between the folding state and function of proteins.

• • How to Select and Optimize Protein Sequencing Methods?

  Protein sequencing is an important analytical technique in the field of bioproducts, which can reveal the amino acid sequence and structural information of proteins, providing key data for protein research and biopharmaceutical development. However, when choosing and optimizing protein sequencing methods, scientists need to consider multiple factors.

• • Analysis of Protein Structure Using Circular Dichroism HT Technology

  Analysis of protein structure is a critical component in the development and production of biologics, and it holds significant importance in understanding protein function and properties. In the field of protein structure analysis for biologic products, high-throughput (HT) circular dichroism spectroscopy (CD) technology is widely utilized.

• • How to Detect Whether a Protein Is Normally Phosphorylated

  Phosphorylation is a critical regulatory mechanism for protein function, influencing numerous biological processes including signal transduction, cell division, and metabolism. To assess whether proteins are phosphorylated, several analytical techniques are employed to identify and quantify their phosphorylation status. Commonly used methods include:

• • Analysis of Protein Secondary Structure Using Circular Dichroism Spectroscopy

  Analysis of protein secondary structure is crucial to revealing the mystery of their folding process. In the field of bioproduct protein structure, circular dichroism (CD) spectroscopy is widely used for analysis of protein secondary structure, revealing the mysteries of protein folding by interpreting CD spectra.

• • Mass Spectrometry Molecular Weight Change of Ubiquitination Sites

  Ubiquitination is a crucial post-translational modification that affects protein stability, activity, and function. This process involves the attachment of a 76-amino-acid ubiquitin molecule to target proteins through a cascade mediated by a trio of enzymes: E1, E2, and E3. Identifying ubiquitination sites is essential for understanding the molecular mechanisms involved. Mass spectrometry is typically employed for this purpose. During ubiquitination, the C-terminal glycine of ubiquitin forms an isopeptide..

• • Do Disulfide Bonds Affect Mass Spectrometry Analysis

  Disulfide bonds can significantly influence protein mass spectrometry analysis, particularly in the study of protein structure and peptide characterization. These covalent bonds, formed between two cysteine residues, play a crucial role in stabilizing the three-dimensional structure of proteins. In mass spectrometry-based proteomics, the presence of disulfide bonds can introduce several challenges: 1. Peptide Digestion Efficiency: Prior to mass spectrometry analysis, proteins are typically digested using...

• • Immunoprecipitation Detects Phosphorylated Proteins

  Immunoprecipitation is a widely used molecular biology technique for studying protein-protein interactions and post-translational modifications. In phosphorylated protein studies, immunoprecipitation enables the selective enrichment of phosphorylated proteins, thereby enhancing signal detection and improving analytical sensitivity.

• • The Purpose of Phosphoproteomics

  Phosphoproteomics is a vital subfield of proteomics that focuses on the study of protein phosphorylation. This post-translational modification is crucial for several biological processes, including cell cycle regulation, signal transduction, gene expression, protein degradation, and immune response. Primary Objectives of Phosphoproteomics: 1. Uncover the Biological Roles of Protein Phosphorylation: Research into the types, distribution, quantitative changes, and functions of phosphorylated proteins is......