Resources

Proteomics Databases



Metabolomics Databases

• • Proteomics Can Analyze Specific Functional Proteins

  Proteomics is a scientific discipline dedicated to investigating protein expression, localization, interactions, and functions within cellular systems. It provides critical insights into the complexity and dynamic nature of biological processes. This paper explores the application of proteomics in the analysis of proteins with defined functional roles. Introduction to Proteomics: Proteins act as crucial functional components across all biological levels, from molecular systems to entire ecosystems. Unlike..

• • Exosome Peptide Detection

  Exosomes are extracellular vesicles secreted by cells throughout their lifecycle. They typically measure between 30 and 150 nanometers in diameter and are enclosed by a phospholipid bilayer, allowing them to exist freely in various body fluids. The cargo of exosomes includes proteins, lipids, RNA, and DNA, rendering them ideal for indicating cellular status and function.

• • Are There Differences in Proteomics Between Serum and Plasma

  In proteomics research, blood samples, including serum and plasma, are widely used biological specimens. Although both are derived from blood, they exhibit key differences in biochemical composition, biological function, and applications in proteomics studies. Definition of Serum and Plasma: Plasma is the liquid component of blood that remains after most red blood cells, white blood cells, and platelets have been removed. It contains a variety of dissolved electrolytes, proteins, hormones, and metabolic....

• • Protein Identification and Quantification by Mass Spectrometry

  Mass spectrometry (MS) is a powerful analytical tool in bioinformatics research, enabling precise identification of protein species and quantification of their abundance. It plays a crucial role in deciphering biological complexity, investigating cellular processes, and advancing disease research. Protein Identification by Mass Spectrometry: Protein identification by mass spectrometry involves detecting and characterizing the proteins present in a given sample. The structural and functional diversity of....

• • Construction of Peptide Library and Mass Spectrometry Analysis

  A peptide library is a diverse collection of peptides that can be generated using multiple approaches, including solid-phase peptide synthesis, enzymatic synthesis, PCR amplification, and recombinant expression under sterile conditions. Construction of Peptide Libraries: Peptide libraries can be constructed using either random or directed synthesis methods. Random synthesis includes both completely random peptide libraries, which encompass all possible amino acid combinations, and partially random..........

• • Chemical Cross-Linking Mass Spectrometry Quality Control

  Mass spectrometry is a powerful analytical technique widely used to determine molecular mass, identify unknown compounds, quantify known compounds, and investigate chemical, biological, and physical processes. Among these approaches, chemical cross-linking mass spectrometry (XL-MS) has emerged as a critical tool for studying protein structures and interactions. However, rigorous quality control is essential to ensure the accuracy and reproducibility of XL-MS data. This review outlines key quality control...

• • Does Protein Degradation Affect Mass Spectrometry Identification

  Mass Spectrometry for Protein Analysis is a crucial tool in biological research, allowing researchers to determine the composition and structural characteristics of proteins. However, protein degradation is a prevalent challenge that can significantly affect the accuracy of mass spectrometry-based protein identification. This article examines the impact of protein degradation on mass spectrometry analysis and discusses strategies to mitigate its effects.

• • What Detector Is Used for Mass Spectrometry in Proteomics

  Mass spectrometry has emerged as a key research area in proteomics. The identification, quantification, and analysis of proteins in complex biological samples rely on mass spectrometry. Within mass spectrometry applications, the selection of an appropriate detector is particularly critical. Electron Multiplier: The electron multiplier is a traditional detector in mass spectrometry, known for its high sensitivity through signal amplification via electron multiplication. It utilizes a high-voltage electric...

• • Histone Methylation Modification Types in Plants

  In botanical research, DNA methylation is a crucial epigenetic modification that regulates gene expression, maintains chromatin stability, and influences various biological processes such as development. In plant genomes, DNA methylation primarily occurs at the fifth carbon position of cytosine (C). Based on the sequence context following the cytosine, DNA methylation can be classified into three types:

• • Precautions and Challenges in Protein Ubiquitination Detection Experiments

  Protein ubiquitination is a post-translational modification process involving the sequential attachment of the small protein ubiquitin to target proteins. This process plays a crucial role in various biological events, including the cell cycle, signal transduction, immune responses, and protein degradation. However, studying protein ubiquitination presents several challenges, as discussed in this paper.