Untargeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service

Untargeted mass spectrometry-based quantitative neuropeptidomics is an advanced analytical technique used to study the dynamic changes of neuropeptides in biological systems. Neuropeptides, as essential signaling molecules in the nervous system, play significant roles in neurotransmission, regulation, and intercellular communication. Due to their low abundance and complexity, traditional analytical methods often struggle to capture the full range of neuropeptide variations. Untargeted mass spectrometry, through full-spectrum scanning, enables comprehensive identification and quantification of neuropeptide diversity.

 



Ibarra, AE. et al. RSC Chem Biol. 2025.

Figure 1. Overview of Mass Spectrometry-based Untargeted Strategies for Neuropeptide Quantification

 

MtoZ Biolabs offers the Untargeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service that is capable of exploring the neuropeptide species in complex samples without pre-selecting target peptides, discovering unknown neuropeptides, and quantifying their roles in disease progression. This service provides valuable data support for neuroscience, clinical research, and drug development.

 

Services at MtoZ Biolabs

MtoZ Biolabs' untargeted mass spectrometry-based quantitative neuropeptidomics service utilizes the most advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology combined with high-resolution mass spectrometry platforms to conduct unbiased and comprehensive analysis of neuropeptides. Through full-spectrum scanning and data-dependent acquisition (DDA) mode, we can identify and quantify unknown neuropeptides from complex biological samples. The core advantage of this service is that it does not require pre-selection of target peptides, enabling a complete capture of neuropeptide molecules in the sample, revealing new neuropeptide biomarkers.

 

For these found neuropeptide biomarkers, MtoZ Biolabs also offers Targeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service, using selected reaction monitoring (SRM) or parallel reaction monitoring (PRM) techniques for precise quantification, providing high-sensitivity and accurate quantitative results. These two services complement each other, offering comprehensive data support for neuropeptide functional research, disease mechanism exploration, and drug development.

 

Analysis Workflow

 

 



 

 

Service Advantages

1. High Sensitivity and Accuracy

We utilize high-resolution mass spectrometry technology, capable of detecting low-abundance neuropeptides from complex biological samples, ensuring the accuracy of quantitative results. Whether for pathological or clinical samples, we provide high-quality data support.

 

2. Support for Multiple Quantification Methods

MtoZ Biolabs' untargeted mass spectrometry-based quantitative peptidomics service supports multiple quantification methods, including Label-Free and Isobaric Labeling techniques such as TMT, iTRAQ, and SILAC. These methods can be selected based on the specific needs of the experiment, ensuring efficient and accurate processing of different samples, and are suitable for multi-sample comparative analysis, low-abundance peptide quantification, and other quantification requirements.

 

3. Flexible Sample Support

Our Untargeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service supports a wide range of sample types, including cerebrospinal fluid, brain tissue, serum, urine, and more, meeting diverse research needs. Whether for basic or clinical research, we offer flexible and precise solutions.

 

4. Highly Specialized Team

MtoZ Biolabs boasts an experienced mass spectrometry analysis team with extensive expertise in neuropeptidomics. We provide comprehensive technical support and data interpretation to ensure the scientific rigor and accuracy of experimental plans.

 

5. Transparent Pricing

We offer a transparent and clear pricing system with no hidden fees. Clients can receive precise quotations based on their needs, helping them plan research budgets and ensuring cost control.

 

Applications

1. Neurodegenerative Disease Research: Neuropeptides play crucial roles in neurodegenerative diseases, depression, Alzheimer's disease, and more. By performing untargeted mass spectrometry quantification of neuropeptides, we can provide strong support for disease mechanism research and early diagnosis.

 

2. Drug Development and Screening: Neuropeptides, as key regulatory factors in the nervous system, are potential drug targets. Through untargeted mass spectrometry analysis, we can assess the impact of drugs on neuropeptides, providing data support for clinical research and drug screening.

 

3. Basic Neuroscience Research: Comprehensive neuropeptide quantification allows in-depth studies of neuropeptide functions in neural signaling, memory, emotional regulation, and other areas, providing essential data support for basic neuroscience research.

 

FAQs

Q: What is the typical timeline for Untargeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service?

A: The timeline for untargeted neuropeptidomics quantitative analysis typically ranges from 2 to 4 weeks, depending on sample type, experimental complexity, and data processing requirements.

 

Sample Submission Suggestions

1. Sample Types: Serum, cerebrospinal fluid, urine, brain tissue, cell culture medium, etc.

2. Sample Volume: We recommend submitting 5-100 µL of sample, depending on the specific experimental requirements.

3. Storage Conditions: Samples should be stored at -80°C and avoid repeated freeze-thaw cycles.

 

Deliverables

1. Quantitative Analysis Report: Includes quantitative results for neuropeptides, statistical analysis, and data visualization charts.

2. Raw Data Files: Provides the original mass spectrometry data files for further analysis.

3. Data Interpretation: Offers a professional report with data interpretation to help clients understand the analysis results.

 

With our cutting-edge mass spectrometry technology and extensive neuropeptide analysis experience, MtoZ Biolabs offers a comprehensive and accurate Untargeted Mass Spectrometry-Based Quantitative Neuropeptidomics Service to provide reliable data support for basic research, drug development, or disease mechanism research.

 

If you are looking for high-quality neuropeptide analysis services, contact us today, and we will provide you with the most professional support for your research projects.