Resources
Proteomics Databases
Metabolomics Databases
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Mass spec phosphorylation analysis refers to the study of protein phosphorylation using advanced mass spectrometry techniques. The process begins with protein extraction from biological samples, such as cells or tissues. Due to the low relative abundance of phosphorylated proteins and the instability of phosphate groups, specialized enrichment methods, such as immunoprecipitation and chemical affinity chromatography, are commonly employed to isolate phosphorylated proteins or peptides. Once enriched......
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• Absolute Protein Quantification
Absolute protein quantification involves determining the precise amount, either in mass or molarity, of proteins within a biological sample. Unlike relative protein quantification, which compares protein levels across different samples, absolute protein quantification provides specific values for protein concentrations. This process typically combines mass spectrometry and isotope labeling techniques to facilitate precise quantification by comparing standards with samples. For instance, in cancer rese......
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The protein-protein interactome represents the intricate network of interactions formed by proteins both inside and outside cells. Proteins are key players in biological processes, forming the core of many life functions through their interactions. These interactions specifically influence processes such as signal transduction, metabolic pathways, cell cycle regulation, gene expression, and immune responses. Research on the protein-protein interactome not only aids in understanding the fundamental mec......
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Oligosaccharide profiling is aimed at thoroughly analyzing the complex structures of oligosaccharides to elucidate their biological functions and roles. Oligosaccharides, composed of a limited number of monosaccharides linked by glycosidic bonds, are prevalent on the surfaces of animal and plant cells and are involved in various biological processes such as cell recognition, immune response, and signal transduction. By comprehensively examining their structures, compositions, and modifications, oligos......
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• Protein Sequencing Technology
Protein sequencing technology is fundamental to determining the amino acid sequences of proteins, providing critical insights into their structure-function relationships. This technology is widely applied in medicine, drug discovery, and biotechnology. In disease research, protein sequencing aids in identifying disease-associated protein variants, supporting early diagnosis and personalized treatment. Furthermore, sequencing specific proteins enables scientists to design engineered proteins with tailo......
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• C-Terminal Protein Sequencing in Biopharmaceuticals
C-Terminal protein sequencing analysis is a technique that precisely analyzes the carboxyl-terminal sequence of proteins or polypeptides. This technique is crucial for identifying C-terminal amino acid sequences, detecting modifications at the C-terminal, and confirming the completeness of protein processing. The C-terminal sequences play significant roles in various biological processes, including the regulation of protein stability, functional activity, interactions, and signal transduction. Moreove......
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Site-specific analysis is employed to investigate genetic or protein sequence variations at the molecular level at specific locations. Its core principle is to identify and analyze variations at precise positions in specific genes or proteins, facilitating insight into gene function, disease mechanisms, and the development of personalized treatment strategies. This method concentrates on the detailed examination of characteristics and changes at specific sites within biological macromolecules (such as......
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• SDS-PAGE Protein Quantification
SDS-PAGE protein quantification analysis is extensively utilized in biochemical and molecular biology research. SDS-PAGE, which stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, is a technique that separates proteins in a sample for analytical purposes. SDS, an anionic detergent, binds to protein molecules, disrupts their native conformation, and confers a uniform negative charge, thereby enabling their migration in an electric field. This technique is broadly employed for protein ......
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Biosimilar characterization involves a thorough and systematic analysis of the physicochemical properties, structural features, functional activity, and safety profiles of biosimilars. This ensures they are highly similar to their reference biologics in terms of quality, safety, and efficacy. Biosimilars, which include complex proteins like monoclonal antibodies, fusion proteins, and recombinant proteins, require meticulous production involving cell cultures and protein purification. Even minor change......
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• Lipid Analysis by Mass Spectrometry
Lipid analysis by mass spectrometry is an indispensable technique for elucidating the composition, structure, and function of lipids. Lipids-including fats, phospholipids, and sphingolipids-are vital for cellular architecture, energy storage, and signal transduction. In lipid analysis by mass spectrometry, lipid molecules are ionized and their mass-to-charge ratios (m/z) are measured, enabling precise determination of molecular masses and structural features. This method is critical for unraveling com......
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