Resources

Proteomics Databases



Metabolomics Databases

• • Expression Analysis and Characterization of Protein

  The expression analysis and characterization of protein encompass the identification, quantification, and functional investigation of proteins within cells or tissues. Proteins, as the primary executors of biological functions, have expression levels and functional statuses that crucially determine the physiological state and responsiveness of cells. This process involves several key aspects. Firstly, quantitative analysis of proteins allows researchers to identify proteins associated with specific fu......

• • Functional Characterization of Proteins

  Functional characterization of proteins seeks to uncover the functions and mechanisms of specific proteins within organisms. Proteins are fundamental to life, participating in nearly all biological processes, such as metabolism, signal transduction, immune responses, and cellular structure maintenance. In the realm of proteomics, understanding protein functions aids scientists in unraveling the complexities of biological systems. This methodology also elucidates protein interactions, regulatory pathwa......

• • N-Terminal Sequencing Method

  The N-Terminal sequencing method is primarily employed to ascertain the amino acid sequence at the N-terminus of proteins or peptides. This method encompasses techniques such as Edman degradation and mass spectrometry analysis. First introduced in the 1950s, the Edman degradation technique determines sequences by systematically removing and identifying the N-terminal amino acids of a peptide chain. Noted for its high specificity and accuracy, it is particularly effective for analyzing short-chain pept......

• • N-Terminal Sequencing Service for Protein Analysis

  The N-terminal sequencing service is a crucial technique for determining the N-terminal amino acid sequences of proteins, which are essential molecules in biological functions. Understanding these sequences provides insights into the proteins' functions, structures, and biological importance. This service aids researchers in identifying the starting sequence of proteins by analyzing the N-terminal sequences of peptides or protein samples. It finds wide-ranging applications in basic research, drug deve......

• • In Vitro Protein-Protein Interaction Assay

  In vitro protein-protein interaction assays are essential for investigating the mechanisms of protein recognition and binding. These assays form the basis for understanding complex biomolecular networks, which is crucial for elucidating intracellular signal transduction, gene regulation, and metabolic pathways. By employing in vitro techniques, scientists can reconstruct functional network maps of cellular processes, enhancing our understanding of disease mechanisms and facilitating the identification......

• • High-Throughput Protein-Protein Interaction

  High-throughput protein-protein interaction refers to the process of efficiently and automatically detecting and analyzing numerous protein interactions using advanced experimental techniques. Proteins are fundamental molecules performing nearly all functions in organisms, and their interactions are crucial for cellular functions, signal transduction, metabolic pathways, and gene regulation. Understanding protein interaction networks is essential for elucidating cellular mechanisms and their alteratio......

• • How to Obtain Comprehensive Protein Information Through de Novo Sequencing

  The goal of de novo sequencing is to analyze the complete amino acid sequence of proteins from mass spectrometry data without bias, including information about mutations, post-translational modifications (PTMs), and isoforms. In order to obtain a comprehensive understanding of protein information, strategies such as optimizing sample preparation, using advanced mass spectrometry techniques, applying multiple fragmentation modes, utilizing intelligent data analysis, and performing PTM analysis must be ......

• • N-Terminal Sequencing: A Comparative Analysis of Edman Degradation and Mass Spectrometry Approaches

  N-terminal sequencing is a widely employed technique for determining the amino acid sequence of proteins. It plays a crucial role in proteomics, molecular biology, and biopharmaceutical research by elucidating protein structural characteristics, modifications, and post-translational processing. The primary methods used for N-terminal sequencing include Edman degradation, a classical chemical approach, and mass spectrometry, a modern analytical technique. These two methods differ markedly in their unde......

• • Studying Protein-Protein Interactions

  Studying protein-protein interactions is a critical research area in both biology and medicine, aimed at understanding the mechanisms of interactions between proteins and their roles in various biological processes. As fundamental biomolecules, proteins perform diverse functions within and outside cells. However, individual proteins often cannot achieve their biological functions independently. Therefore, studying protein-protein interactions is essential for gaining insights into the complex molecula......

• • Measuring Protein-Protein Interactions

  Measuring protein-protein interactions is a crucial technique in biochemistry and molecular biology, used to explore how proteins interact and to elucidate their roles within the cell. This approach enhances our understanding of cellular signal transduction, metabolic pathways, gene expression regulation, and the molecular mechanisms underlying various diseases. As proteins are essential for life processes, they often require interactions with other proteins or biomolecules to function optimally. Such......