Resources
Proteomics Databases
Metabolomics Databases
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O-glycosylation detection is essential for understanding one of the most prevalent forms of post-translational modification (PTM) found in proteins and peptides. O-glycosylation involves the covalent attachment of a sugar moiety—commonly N-acetylgalactosamine (GalNAc), galactose, or xylose—to the hydroxyl group of serine (Ser) or threonine (Thr) residues via an O-glycosidic bond. This modification plays a critical role in regulating protein function, stability, and intercellular communication. Aberran......
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• Cross-Linking Mass Spectrometry Techniques
Cross-linking mass spectrometry techniques integrate chemical cross-linking with mass spectrometry analysis to investigate spatial interactions between proteins and to elucidate the architecture of protein complexes. By applying cross-linkers containing reactive groups that form stable covalent bonds between neighboring amino acid residues within or between proteins, XL-MS enables the capture and identification of these interactions. This, in turn, provides valuable insights into the three-dimensional......
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• Advances in Protein Research: Recombinant Expression, Purification, and Identification
In the field of biopharmaceuticals, proteins play a critical role in drug development processes. Through protein research, scientists can better understand protein structures and functions, and assess whether a given protein meets specific therapeutic or experimental requirements. Recent studies have reported significant advances in the areas of recombinant protein expression, purification, and identification. This article provides a detailed overview of these developments, with a particular focus on ......
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• Post-IP Mass Spectrometry Analysis
Post-IP mass spectrometry analysis is a powerful integrative technique for identifying the components of protein complexes and investigating protein–protein interactions. This approach begins with the immunoprecipitation (IP) of a target protein and its potential interaction partners using a specific antibody, followed by mass spectrometry (MS) to identify the co-precipitated proteins and examine their potential post-translational modifications. The key steps and considerations of this workflow are ou......
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• Determination of Protein Molecular Weight by Electrophoresis
The determination of protein molecular weight by electrophoresis is a widely employed laboratory technique, most commonly performed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). This method leverages the ability of SDS (sodium dodecyl sulfate) to bind uniformly to proteins, conferring a consistent negative charge and enabling separation based on molecular weight. The following outlines the fundamental principles and procedural steps of this method: Principle 1. Role of ......
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Targeted drug discovery constitutes a critical component of modern pharmaceutical research and development. This approach is centered on identifying molecular targets that play pivotal roles in the initiation and progression of diseases and designing therapeutics with high specificity toward these targets. Unlike traditional drug development strategies that rely heavily on empirical screening, targeted drug discovery is grounded in molecular mechanisms and emphasizes precise intervention along key .........
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• High Throughput Single Cell Sequencing
High throughput single cell sequencing enables comprehensive profiling of the genome, transcriptome, and epigenome at the resolution of individual cells. This technique has demonstrated considerable utility in medical research, particularly in oncology. Tumors typically consist of highly heterogeneous cell populations. By applying single cell sequencing, researchers can identify distinct subpopulations within tumors and gain deep insights into their unique gene expression profiles and mutational landscapes.
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Biomarker discovery methods encompass a suite of technologies and strategies aimed at identifying and validating biomolecules indicative of specific biological states or disease conditions. These biomarkers may include genes, proteins, metabolites, or even cellular-level alterations. Biomarker discovery methods involve a broad range of techniques from genomics, proteomics, metabolomics, and bioinformatics. In genomics, frequently employed approaches include genome-wide association studies (GWAS) and .......
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Molecular glue screening is a systematic strategy for identifying or optimizing small molecules that can induce or enhance protein–protein interactions (PPIs). Unlike traditional inhibitors or agonists that typically act on a single target, molecular glues are a unique class of small molecules that function as “molecular bridges,” selectively facilitating the interaction between two proteins. This interaction can modulate cellular processes or even trigger the degradation of target proteins. By.........
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The E. coli HCP assay is a critical analytical procedure used for the qualitative and quantitative assessment of residual non-target proteins—i.e., host cell-derived impurities—within the E. coli expression system during recombinant protein production. This procedure plays a pivotal role in ensuring the safety, purity, and consistency of biopharmaceutical products. Due to its well-characterized genetics, rapid growth, and high protein expression yields, E. coli is widely employed as a host organism in......
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