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Proteomics Databases



Metabolomics Databases

• • 4 Effective Edman Degradation Tips to Improve Protein Analysis Accuracy

  Edman degradation has remained a cornerstone technique for N-terminal protein sequencing since its introduction in the 1950s. It continues to play a pivotal role in studies of protein function, identification of novel proteins, and antibody validation. While modern mass spectrometry techniques have become prevalent in proteomics, Edman degradation retains unique advantages in precisely determining the N-terminal amino acid sequence. However, its effectiveness is highly contingent upon meticulous.........

• • Principle, Advantages and Disadvantages of Edman Sequencing

  Edman sequencing is a chemical method that sequentially analyzes the N-terminal amino acid sequence of proteins and is widely used in the investigation of protein primary structure. Although modern mass spectrometry techniques have become the dominant tools in proteomics, Edman sequencing maintains distinct advantages in specific research contexts due to its high specificity and ability to directly determine amino acid sequences. This paper systematically outlines the fundamental principle, as well as the..

• • How to Achieve High-Efficiency Protein Sequence Analysis with Edman Sequencing? A Lab Guide

  Edman degradation is a classical and highly specific technique for protein N-terminal sequence analysis, which continues to play a pivotal role in structural proteomics, antibody engineering, and biopharmaceutical research. Although high-resolution mass spectrometry technologies have advanced rapidly, Edman degradation retains unique, irreplaceable value in specific applications due to its ability to sequentially resolve N-terminal amino acids. This article provides a comprehensive laboratory guide on......

• • Struggling with N-Terminal Protein Sequencing? 2 Common Issues and How to Fix Them!

  In proteomics, N-terminal protein sequencing plays a critical role in elucidating the initiation sites of proteins, post-translational modifications (PTMs), signal peptide cleavage, and protein degradation pathways. However, a frequent technical challenge encountered by researchers is the inability to detect the N-terminal protein using standard methods. This article explores the mechanistic basis of two major technical bottlenecks in N-terminal protein sequencing and presents practical solutions to .......

• • Edman Sequencing vs. Mass Spectrometry: A Comparison of Protein Sequencing Techniques II

  Proteins, as central functional molecules within cells, carry amino acid sequence information that not only elucidates their structure and function but also underpins various applications such as biomarker discovery, antibody characterization, and vaccine development. As a fundamental technique for deciphering amino acid composition and sequence, protein sequencing has evolved over several decades into two dominant methodologies: Edman sequencing and Mass Spectrometry (MS). What are the unique advantages...

• • APEX Proximity Labeling

  APEX proximity labeling, utilizing engineered ascorbate peroxidase (APEX), is a robust method for investigating protein interactions and subcellular localization within living cells. The central component of this technique is the modified peroxidase APEX, which is fused to target proteins or cellular structures to facilitate rapid and covalent labeling of proximal proteins. When compared to traditional approaches like immunoprecipitation and yeast two-hybrid methods, APEX proximity labeling offers enh......

• • Proteomics in Biotechnology

  Proteomics in biotechnology is a discipline that focuses on the comprehensive study of the proteome—the complete set of proteins expressed by an organism—encompassing their structures, functions, dynamic alterations, and interactions. Proteins are fundamental to life, executing essential cellular functions such as catalyzing metabolic reactions, DNA replication, and signal transduction. The advent of proteomics enables researchers to systematically explore the intricate network of proteins and their r......

• • Western Blot Detection of Histone Methylation

  Western blot detection of histone methylation is a crucial approach in epigenetics, enabling researchers to investigate how histone modifications regulate chromatin structure and transcriptional activity. Histone methylation is a form of post-translational modification that critically influences gene expression programs. Catalyzed by histone methyltransferases (HMTs), this process involves the enzymatic transfer of methyl groups from the donor S-adenosylmethionine to lysine residues on histone tails.

• • Websites for Analyzing Glycosylation Sites

  Various websites for analyzing glycosylation sites have been developed to support the accurate prediction of glycosylation patterns in proteins. These platforms offer computational tools for identifying potential N-, O-, and C-linked glycosylation, as well as non-enzymatic glycation and GPI anchoring. Below are some widely used and well-documented resources that enable researchers to streamline their glycosylation analysis. NetNGlyc & NetOGlyc 1. Website: NetNGlyc and NetOGlyc 2. Function: These tools are..

• • Amino Acid Full Sequence Analysis

  Amino acid full sequence analysis serves as a foundational approach for understanding protein function, beginning with the elucidation of the primary structure—the linear arrangement of amino acids connected by peptide bonds to form a polypeptide chain. This initial level of analysis provides critical insights into the molecular architecture and biological roles of proteins and typically involves the following steps: 1. Determination of the Amino Acid Sequence The amino acid sequence is established using...