Resources
Proteomics Databases
Metabolomics Databases
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Isobaric mass spectrometry is an advanced analytical technique that uses isotopic labels to precisely measure and compare the molecular weights of compounds in a sample. By utilizing isobaric mass spectrometry, researchers can identify and quantify proteins in complex biological samples, revealing their expression levels and interactions within organisms. One of the primary applications of this technique is in the identification and validation of disease biomarkers. Isobaric mass spectrometry aids in ......
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ICP-MS metal analysis is an advanced analytical technique that combines inductively coupled plasma (ICP) and mass spectrometry to detect and measure metals and select non-metallic elements in samples. The core principle involves ionizing elements through ICP, which is then analyzed using a mass spectrometer. ICP-MS plays a pivotal role across various fields, including environmental monitoring, food safety, biomedical research, geological studies, and materials science. In environmental monitoring, I......
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GC-MS metabolomics is an advanced analytical technique that combines gas chromatography and mass spectrometry to systematically investigate small molecules in living organisms. These metabolites are key players in many biological processes and physiological functions. This technology not only characterizes the composition of metabolites but also reveals their changes under different physiological conditions, making it a powerful tool for understanding metabolic pathways and biochemical reactions in or......
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Stable isotope labeling is a commonly used technique in biomolecular research, enabling precise tracing and quantification of biomolecules. This method relies on isotopes that share the same number of protons but differ in neutron count. While these isotopes exhibit nearly identical chemical properties, their subtle differences in physical properties allow for effective differentiation using mass spectrometry and other analytical techniques. Stable isotope labeling has broad applications, particular......
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SRM mass spectrometry is a widely utilized analytical technique in proteomics research. Its development dates back to the mid-20th century, and with continuous advancements in mass spectrometry technology, it has been progressively refined. Today, it is an essential tool in many laboratories, serving as a powerful analytical method in scientific research. The applications of SRM mass spectrometry span multiple fields, including biomedical research, drug metabolism studies, environmental monitoring, an......
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MRM assay is a mass spectrometry-based quantitative approach characterized by high sensitivity and specificity. It operates through a tandem scanning mode in triple quadrupole mass spectrometry, where a precursor ion is selected, fragmented via collision-induced dissociation (CID) to produce a specific product ion, and subsequently analyzed through designated ion transitions for highly selective quantification. Compared to traditional full-scan mass spectrometry, MRM assay provides superior selectivit......
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• Small Molecule Target Identification
Small molecule target identification is the process of identifying the biological targets of small molecule compounds using experimental techniques or computational approaches, typically involving biomolecules such as proteins, DNA, or RNA. Small molecule drugs play a central role in modern drug development, primarily by binding to specific targets and modulating biological processes to achieve therapeutic effects. However, during drug screening, the precise molecular targets of small molecules are of......
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Off-target profiling is a critical methodology for investigating the unintended interactions of drugs, chemical compounds, or biologics with non-target molecules in vivo and in vitro. As a fundamental aspect of drug development and safety evaluation, off-target profiling aims to identify potential nonspecific interactions to mitigate adverse effects, refine drug design, and improve drug safety profiles in clinical applications. While small-molecule drugs, antibody-based therapeutics, and nucleic acid ......
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• Phage Display Protein-Protein Interactions
Phage display protein-protein interactions represent a molecular biology approach that employs phage display technology to investigate interactions between proteins, protein-small molecules, and protein-antibodies. This technique utilizes bacteriophages, primarily M13, T7, or λ phages, as vectors to display exogenous proteins or peptide fragments on the surface of phage coat proteins, enabling specific recognition and selection of target proteins. Phage display has been widely applied in the developme......
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Peaks de novo sequencing is a computational method for deriving protein or peptide amino acid sequences directly from mass spectrometry data without relying on known protein sequence databases. In proteomics research, conventional protein identification approaches primarily rely on database-dependent searches, where experimental peptide mass spectra are matched against pre-existing protein databases. However, this approach is inherently limited when analyzing non-model organisms, novel proteins, antib......
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