Resources

Proteomics Databases



Metabolomics Databases

• • Commonly Used Proteases in Protein Sequencing

  Protein sequencing refers to the process of determining the amino acid sequence of a protein. Commonly used proteases in protein sequencing are essential to this process, as they enzymatically cleave proteins into smaller peptide fragments suitable for analysis. Below are commonly used proteases and their detailed characteristics: Trypsin 1. Cleavage Specificity Trypsin cleaves peptides at the carboxyl side of lysine (K) and arginine (R) residues. 2. Key Features As the most widely used protease in ........

• • Microtubule Protein Acetylation Detection

  Microtubule protein acetylation detection is a key analytical approach used to assess the post-translational modification status of tubulin proteins, which are critical structural components of the cytoskeleton. Microtubules are primarily composed of α- and β-tubulin heterodimers and play essential roles in various cellular processes. 1. Maintenance of Cell Morphology Microtubules provide essential structural support that helps preserve cellular shape and mechanical stability. 2. Intracellular Transport....

• • Quantitative Determination of Immunoglobulins

  Commonly Used Determination Methods 1. Nephelometry and Turbidimetry These two methods rely on the principle that antigen-antibody complexes increase the turbidity of a solution. The concentration of immunoglobulins is quantified by measuring either the intensity of light scattering (nephelometry) or absorbance (turbidimetry) in the reaction mixture. 2. Enzyme-Linked Immunosorbent Assay (ELISA) This method utilizes enzyme-conjugated specific antibodies. Upon binding to the target immunoglobulin, the .......

• • Methods of Proteolytic Digestion in Proteomics

  Proteolytic digestion plays a pivotal role in proteomics by breaking down proteins into smaller peptide fragments, enabling comprehensive downstream analysis and accurate protein identification. This procedure is essential for elucidating protein structure and function, as well as understanding their involvement in various biological processes. The following is a detailed overview of commonly used methods for proteolytic digestion: Preparation Work 1. Protein Sample Preparation The initial step involves....

• • Epitope Mapping of Antigen-Antibody Binding

  Epitope mapping of antigen-antibody binding is a technique used to identify specific regions on an antigen molecule that are recognized by antibodies. An epitope, also referred to as an antigenic determinant, is a particular site on the antigen that is bound specifically by an antibody. Epitopes can be either linear, comprising a continuous stretch of amino acids, or conformational, formed by non-contiguous residues that come into proximity through the folding of the antigen’s three-dimensional structure.

• • Determination of Disulfide Bond Content

  The determination of disulfide bond content is essential for understanding protein structure and function. Disulfide bonds are covalent linkages formed between the sulfur atoms of two cysteine residues. These bonds play a crucial role in maintaining the three-dimensional structural integrity of many proteins. Accurate quantification of disulfide bond content is a fundamental aspect of biochemical research. The following methods are commonly employed for disulfide bond determination:

• • Glycation Product Analysis

  Glycation product analysis is essential for understanding the formation, detection, biological effects, and clinical applications of glycation-derived compounds. The following are several key aspects of glycation product analysis: Types of Glycation Products 1. Early Glycation Products These compounds are formed at the initial stage of glycation and are typically reversible in nature. 2. Advanced Glycation End Products (AGEs) These are glycation-derived compounds that accumulate chronically within the .....

• • Determination of Ubiquitination Sites

  The identification of ubiquitination sites is critical for understanding the regulatory roles of ubiquitin in diverse cellular processes. Ubiquitination is a post-translational modification (PTM) in which the small regulatory protein ubiquitin is covalently attached to lysine residues of substrate proteins. Bioinformatics Analysis 1. Database Search Publicly available protein and ubiquitination site databases (e.g., Ubiquitin Ligase Database, UbiSite, PhosphoSitePlus) are used to identify potential ........

• • Analysis of Antibody Variable Regions

  The analysis of antibody variable regions represents a critical research focus in the fields of immunology and biotechnology. This analysis aims to characterize the structural and functional properties of the antibody domains responsible for antigen recognition and binding, specifically the variable regions. Structure of Antibody Antibodies are large glycoproteins composed of two heavy chains and two light chains, each containing a variable region (V region) and a constant region (C region). Located at.....

• • Liquid Chromatography for Protein Detection Technology

  Liquid chromatography (LC) is an analytical technique widely employed for the separation, identification, and quantification of components within complex mixtures. In the field of protein analysis, LC plays a critical role in resolving structural and compositional heterogeneity. Common LC techniques used in protein analysis include Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), Ion Exchange Chromatography (IEC), Hydrophilic Interaction Liquid Chromatography (HILIC), and Gel Permeation .....