Peptide Structure Analysis Services
- Drug Development and Generic Consistency Evaluation
- Structure-Function Relationship Studies
- Protein Engineering and Peptide Optimization
- Vaccine Design and Immunopeptide Screening
Peptide Structure Analysis Services refer to professional technical services that use a variety of structural analysis methods (such as circular dichroism, nuclear magnetic resonance, high-resolution mass spectrometry, etc.) to comprehensively analyze and model the spatial conformation of peptide molecules. These services provide detailed data on secondary structure composition, three-dimensional conformational information, disulfide bond connectivity, and conformational isomerism, supporting researchers and drug developers in understanding the structural basis and functional relevance of peptides.
As the fundamental structural and functional units of proteins, peptide molecules are widely present in organisms and are involved in key physiological processes such as signal transduction, immune regulation, and enzyme inhibition. Their biological activity is not only determined by the amino acid sequence but is also closely related to their spatial conformation. In peptide drug development, vaccine design, and mechanistic studies, structural information is essential for understanding peptide stability, target affinity, and biological function. Compared to larger proteins, peptides typically have shorter chain lengths and greater structural flexibility, making them more susceptible to changes in pH, temperature, and solvent conditions. Therefore, systematically analyzing the structural information of peptides, such as secondary structure (such as α-helix, β-fold), tertiary structure (such as disulfide bonds), and conformational isomers, not only helps to accurately evaluate their biological activity and stability, but also provides an important basis for optimizing peptide drug formulations, developing functional peptide preparations, and realizing structure-function prediction.
Services at MtoZ Biolabs
MtoZ Biolabs offers a comprehensive portfolio of Peptide Structure Analysis Services designed to elucidate the structural features of peptides. By integrating advanced technologies such as CD, NMR, and MS, we provide high-resolution structural data to support peptide drug development, formulation stability studies, and functional characterization. Our core structural analysis services include:
NMR-Based Peptide Structure Analysis
Utilizing high-resolution nuclear magnetic resonance, this service provides atomic-level structural information of peptides in solution. It reveals peptide chain conformations, hydrogen bonding networks, hydrophobic regions, and steric hindrance features.
Peptide Disulfide Bond & Free Cysteine Detection
Through a combination of MS, chromatography, and CD spectroscopy, this service comprehensively analyzes disulfide bonds and free cysteine residues in peptides. It uncovers the bonding state of disulfide linkages, the concentration of free cysteine, and their potential impact on peptide structure and biological activity.
Peptide Disulfide Bond Quantitative Analysis
Using quantitative mass spectrometry techniques, this service precisely quantifies the number, distribution, and redox state changes of disulfide bonds within peptide molecules.。
CD-Based Peptide Secondary Structure Analysis
CD spectroscopy is used to determine the typical secondary structures of peptides, such as α-helices, β-sheets, or random coils, and to monitor their structural stability under various environmental conditions.
Miles AJ. et al. Chem Soc Rev. 2016.
Figure 1. Circular dichroism spectra typical of membrane proteins composed of different secondary structural types.
Service Advantages
Integrated Multi-Platform Analysis: Combining CD, NMR, and mass spectrometry technologies, this service enables in-depth, multi-level structural characterization of peptides-from primary sequence to three-dimensional conformation.
High Sensitivity and High Resolution: Powered by advanced instrumentation, the platform delivers clear and reliable structural data even for low-concentration samples or peptides with complex conformational profiles.
Expert Data Interpretation Team: Our team brings extensive experience in peptide structural analysis, ensuring scientifically sound and reliable interpretation of results.
Customized Service Support: Experimental workflows can be tailored to specific research objectives, including stability assessment, conformational dynamics, and more.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Peptide Structure Analysis Services data, providing clients with a comprehensive data report.
Sample Submission Suggestions
Sample Types: Supports synthetic peptides, naturally sourced peptides, recombinant expressed peptides, etc.
Storage and Transportation: Store at low temperatures (−20°C or −80°C). Dry ice transportation is recommended to avoid repeated freeze-thaw cycles.
Additional Notes: We recommend contacting us prior to sample submission for detailed and tailored sample preparation guidelines.
Applications
Examples of applications for Peptide Structure Analysis Services:
Characterize peptide drug conformations and critical disulfide bond linkages to support quality control and bioequivalence assessment of generic formulations.
Investigate the mechanistic relationship between specific conformations and biological functions such as receptor binding or enzyme inhibition.
Facilitate the design of peptide variants with improved stability, activity, or target-binding affinity through structural modification.
Optimize the conformational presentation of immunogenic epitopes to enhance antigen recognition and immune response.
Related Services
Peptide Sample Preparation Service
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