Peptide Sample Preparation Service
- Peptidomics Research
- Structural Biology Research
- Bioreactivity and Efficacy Evaluation
Peptide Sample Preparation Service refers to a series of technical processes used to extract, digest and purify peptide molecules from complex biological samples (such as cells, tissues, serum, urine, etc.), ensuring that these peptide samples are suitable for subsequent mass spectrometry analysis, post-translational modification analysis, and other biological research. The goal of this service is to provide high-quality, high-purity peptide samples for downstream analysis, helping researchers accurately obtain structural information, functional characteristics, and post-translational modification data of peptides.
With the advancement of biotechnology, the application of peptides in fields such as proteomics, drug development, and vaccine research has become increasingly widespread. The function and biological activity of peptides largely depend on their structure, which is influenced by factors such as amino acid residues, post-translational modifications, and disulfide bonds. Peptide sample preparation is a critical step in peptide structure and activity research, directly affecting the accuracy of subsequent analyses and the reliability of the data. Therefore, an accurate and standardized sample preparation process is a prerequisite for peptide mass spectrometry analysis, post-translational modification analysis, and peptideomics research.
Using advanced technological platforms and specialized experimental techniques, MtoZ Biolabs provides the Peptide Sample Preparation Service to extract, digest, and purify peptide molecules from complex biological samples, offering high-quality peptide samples for subsequent peptideomics and other research.
Analysis Workflow
The main process of the Peptide Sample Preparation Service is as follows:
1. Peptide Extraction
Choose the appropriate extraction method (such as solvent extraction, ultrasonic disruption, etc.) based on the sample type to extract target peptides from complex biological samples.
2. Enzymatic Digestion
Use appropriate enzymes (such as trypsin) to digest the extracted sample, generating small peptides suitable for mass spectrometry analysis. Enzymatic digestion conditions (such as time, pH, and temperature) are carefully controlled to maintain peptide stability.
3. Peptide Purification
Employ techniques such as reverse-phase chromatography and ion exchange chromatography to purify the digested peptide sample, removing impurities and unreacted materials, ensuring high peptide purity.
4. Peptide Concentration Confirmation
Use a spectrophotometer or mass spectrometry-based quantification methods to confirm the peptide concentration, ensuring the sample meets the requirements for subsequent analysis.
Palanski BA. et al. Nat Commun. 2022.
Figure 1. An efficient chemical extraction and LC–MS/MS workflow for urinary peptidomic analysis.
Service Advantages
Advanced Analysis Platform: MtoZ Biolabs established an advanced Peptide Sample Preparation Service platform, guaranteeing reliable, fast, and highly accurate service.
High Purity and Quality: Through optimized extraction, digestion, and purification processes, the service ensures high purity of peptide samples, preserving their structure and function to the greatest extent.
Customized Service Solutions: Based on the client's research objectives and sample characteristics, personalized sample preparation plans are provided to ensure optimal experimental results.
Subsequent Analysis Services: A range of analytical services, including sequence analysis, post-translational modification analysis, structural analysis, and omics analysis, can be provided as needed.
Sample Submission Suggestions
Sample Types: Supports a variety of sample types, including cells, tissues, and body fluids.
Storage and Transportation: Store at low temperatures (−20°C or −80°C). Dry ice transportation is recommended. Avoid repeated freeze-thaw cycles.
Additional Notes: We recommend contacting us prior to sample submission for detailed and tailored sample preparation guidelines.
Applications
Peptide Sample Preparation Service can be applied to various research fields, including but not limited to:
By optimizing peptide sample preparation, the service enhances the accuracy and sensitivity of analyses, providing high-quality data for peptide identification, quantification, and post-translational modification studies.
High-quality peptide sample preparation aids in the three-dimensional structural analysis of proteins, especially in studies of protein folding, functional research, and molecular dynamics simulations, providing accurate structural information.
Provides precise peptide samples for drug efficacy studies, supporting the evaluation of drug bioreactivity and efficacy, and offering experimental data support for drug development and optimization.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
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