Immunoprecipitated Protein Identification Service
Immunoprecipitated protein identification service performs high-resolution mass spectrometry-based identification of proteins from immunoprecipitated samples. The workflow typically includes protein digestion, peptide separation, mass spectrometry detection, and database searching, ultimately generating reliable protein identification results. Immunoprecipitated protein identification service can not only confirms the identity of target proteins but also systematically characterizes their interacting protein networks, identify potential signaling pathway members, regulatory factors, or disease-associated molecules, thereby providing important insights into protein function, novel drug target discovery, and disease mechanism studies.
Figure 1. Workflow of Immunoprecipitated Protein Identification Based on HPLC-MS
MtoZ Biolabs offers a professional immunoprecipitated protein identification service which is based on high-resolution mass spectrometry platforms to provide accurate and reliable analysis of various immunoprecipitated samples. By performing LC-MS/MS detection and database searching of target proteins and their interacting partners enriched by specific antibodies, we provide clients with high-confidence protein identification and comprehensive complex composition analysis. Our service includes but is not limited to:
1. Tagged IP proteins identification
2. Co-immunoprecipitated proteins identification
3. Standard immunoprecipitated proteins identification
4. RNA immunoprecipitated (RIP) proteins identification
5. Chromatin immunoprecipitation (ChIP) proteins identification
Experimental Instruments
Thermo Scientific Q Exactive
Thermo Scientific Orbitrap Fusion Lumos
Electrospray Ionization Mass Spectrometry (ESI-MS)
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOF)
Service Advantages
1. Efficient Turnaround Time
MtoZ Biolabs adopts standardized experimental workflows and professional project management, significantly shortening the turnaround time while ensuring data quality. We provide full-process analysis from sample testing to final report delivery, supporting both research schedules and drug development timelines.
2. Data Reliability and Quality Assurance
A comprehensive quality control system is implemented from sample preparation to mass spectrometry data acquisition. Combined with multi-database cross-validation, this ensures high-confidence and reproducible protein identification results.
3. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Sample Submission Suggestions
1. Quality Verification: Before submitting immunoprecipitated samples for mass spectrometry analysis, approximately 10% of the eluate should be run on an SDS-PAGE gel, stained with Coomassie, and validated by Western blot to confirm the presence of the bait protein or other positive controls. Please send these gel and blot images to our technical team for confirmation before submitting the remaining 90% of the samples for LC-MS/MS analysis.
2. Experimental Replicates: To ensure statistical significance, we recommend at least three biological replicates with appropriate controls.
3. Sample Specifications: All tubes must be clearly labeled and properly sealed. Submission instructions must include detailed sample information such as species name, experimental conditions, and other relevant details.
4. Shipping Conditions: Samples must be shipped with sufficient dry ice to ensure low-temperature preservation and prevent degradation during transport.
Deliverables
1. Detailed description of sample processing and experimental workflow (including digestion, separation, and MS parameters)
2. Raw mass spectrometry data files (RAW/mzML format)
3. Protein identification list (including protein names, database matching scores, coverage, and confidence levels)
4. Protein–protein interaction network analysis figures and functional annotation results
5. Standardized technical report (PDF), including methods, results, discussion, and data interpretation
MtoZ Biolabs is committed to providing a high-quality and reliable immunoprecipitated protein identification service for researchers and biopharmaceutical companies. With advanced mass spectrometry platforms, a robust quality system, and an experienced technical team, we deliver comprehensive and precise protein identification and interaction network analysis for immunoprecipitated samples, empowering deeper exploration of protein function and disease research. Whether for basic research or drug development, our efficient and professional services will support your scientific progress.
Contact us today and let MtoZ Biolabs be your trusted scientific partner.
Related Services
Protein Identification Service
Protein Identification Service by Tandem Mass Spectrometry
Co-Immunoprecipitation Protein Interaction Analysis Service
Immunoprecipitation Analysis Service
Large-Scale Protein Identification Service
How to order?
