Immunoprecipitation Analysis Service
- Western Blotting (WB): Confirm the presence and purity of the target protein.
- Enzyme-Linked Immunosorbent Assay (ELISA): Quantify variations in target protein abundance.
- Mass Spectrometry (MS): Characterize the protein in-depth and confirm its identity.
- Functional Assays: Assess the biological activity of the purified protein, such as enzyme activity or signaling function.
Immunoprecipitation (IP) is a well-established technique used to selectively enrich and purify target proteins from complex biological samples. By employing specific antibodies, IP allows for the capture of a target protein from cell lysates, tissue extracts, or other biological matrices. This method is crucial for studying protein expression, post-translational modifications, and protein function. As a core technique in proteomics, IP has wide applications in areas such as signal transduction, disease mechanisms, and drug discovery.
MtoZ Biolabs offers a precise and efficient Immunoprecipitation Analysis Service, utilizing advanced instrumentation and the expertise of our professional team. Our end-to-end solutions cover the entire immunoprecipitation workflow, from experimental design and antibody selection to downstream analysis and data interpretation, providing a seamless experience for researchers.
Technical Principles
Immunoprecipitation relies on the high-affinity and specificity binding between antibodies and target proteins. In the process, antibodies form stable complexes with target proteins, which are then captured using magnetic or agarose beads (e.g., Protein A/G beads). This selective enrichment allows for the isolation of the target protein from complex biological samples. After several washing steps to remove nonspecific bindings, high-purity target protein is obtained through optimized elution conditions. The purified protein can be analyzed using techniques such as Western blotting, ELISA, or mass spectrometry to gain deeper insights into its characteristics and functions.
Analysis Workflow
To ensure high precision and reproducibility, MtoZ Biolabs' Immunoprecipitation Analysis Service follows a carefully designed workflow. Each step is optimized for efficiency and tailored to meet specific research needs, ensuring reliable results every time.
1. Experimental Design and Antibody Selection
Select high-specificity antibodies and optimize experimental conditions based on the client’s research goals, ensuring efficient and specific antibody binding to the target protein.
2. Sample Lysis and Pre-treatment
Lyse cell or tissue samples using optimized buffers that maintain protein complex stability while minimizing nonspecific binding, ensuring the best possible results for target protein enrichment.
3. Immunoprecipitation
Enrich the target protein by binding it with antibodies and magnetic beads. This step isolates the target protein from the complex biological matrix, ensuring a high degree of specificity.
4. Elution and Separation
Employ gradient elution techniques to remove nonspecific binding components and obtain high-purity target protein. Optimized elution conditions ensure the recovery of a clean and concentrated sample.
5. Downstream Analysis
Service Advantages
1. Optimized Enrichment: Carefully selected antibodies and experimental conditions ensure efficient target protein enrichment with minimal background.
2. High Sensitivity: The rigorous washing and elution steps ensure that only high-purity target proteins are captured, providing reliable and reproducible results.
3. Flexible Analysis: Supports various detection methods, including WB, ELISA, MS, and functional assays, meeting diverse research objectives.
4. Comprehensive Support: We provide end-to-end technical support, from design through to data interpretation, ensuring high-quality and reliable results.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Case Study
Case1: Isolating Nanoparticles from Complex Media Using Immunoprecipitation
This study used immunoprecipitation analysis technology to enrich target nanoparticles and their associated protein complexes from complex media like plasma and cell lysates. Surface protein characterization revealed critical interactions between nanoparticles and biological systems, providing key support for nanoparticle drug delivery research.
Hacene, Y. C. et al. Nano Lett.. 2021.
Case2: Efficiently Enriching ALS-related TDP-43 Aggregates Using Tandem Washing and Immunoprecipitation
By combining immunoprecipitation analysis and tandem washing, this study successfully enriched TDP-43 protein aggregates from ALS patient samples, enhancing both recovery and purity. Findings highlighted the central role of TDP-43 in neurodegenerative disease pathology, offering new drug development targets.
Evangelista, B. A. et al. iScience. 2023.
FAQ
Q1: How do you select the right antibody to enhance immunoprecipitation analysis specificity and sensitivity?
Antibody specificity and affinity are critical in determining enrichment efficiency and signal intensity. MtoZ Biolabs selects the best antibodies through comprehensive research, combining prior literature and experimental validation to ensure stable, high-purity protein complexes for reliable downstream analysis.
Q2: How do you minimize nonspecific binding and background interference?
We reduce nonspecific binding by optimizing buffer systems, salt concentrations, and washing conditions. Our standardized experimental workflows, including control strategies, enable rapid identification and reduction of background signals, ensuring the reliability of results.
Choosing MtoZ Biolabs for your immunoprecipitation analysis needs guarantees a full-service experience, from design and execution to data analysis. With advanced platforms, expert teams, and customizable solutions, we deliver high-quality, reliable results to support your research and act as your trusted long-term partner.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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