Histone PTM Identification Service
- We accept various sample types, including tissue, cells, body fluids, protein extracts, and other biological samples.
- Samples should be stored at -80℃ and shipped with dry ice.
Histone post-translational modifications (PTMs) play a pivotal role in regulating chromatin dynamics, gene expression, and cellular identity. To support cutting-edge epigenetics research, MtoZ Biolabs offers a high-resolution Histone PTM Identification Service that enables precise mapping and quantification of histone modifications across a wide range of biological contexts.
Histones are subject to a wide array of PTMs, including acetylation, methylation, phosphorylation, ubiquitination, and sumoylation. These modifications primarily occur on the N-terminal tails of histone proteins and play essential roles in DNA accessibility, transcriptional activation or repression, and higher-order chromatin organization. The spatial and combinatorial patterns of histone PTMs constitute a dynamic "histone code" which can modulate cellular phenotypes and developmental programs. Aberrant histone PTM patterns are closely associated with diseases such as cancer, neurodegeneration, and immune disorders, making their characterization essential for both basic research and therapeutic development.
Figure 1. Post-Translational Modifications (PTMs) at Histone Tails and Their Impact on Chromatin Remodelling
Service at MtoZ Biolabs
Leveraging advanced LC-MS/MS platforms, optimized peptide enrichment strategies, and robust bioinformatics pipelines, MtoZ Biolabs' Histone PTM Identification Service delivers comprehensive profiling of histone PTMs with site-level resolution, quantitative depth, and functional insight. Our end-to-end workflow integrates sample preparation, mass spectrometry analysis, and downstream bioinformatics interpretation to provide actionable insights into histone modification landscapes. We are capable of identifying a wide array of histone modifications, including but not limited to phosphorylation, acetylation, and methylation, enabling in-depth investigation of chromatin dynamics and epigenetic regulation. Whether you are studying gene expression control, disease mechanisms, or therapeutic interventions, our histone PTM analysis solutions are designed to accelerate your discoveries.
Analysis Workflow
1. Sample Preparation: Histone proteins are extracted from cells or tissues using optimized protocols to preserve labile modifications.
2. Enzymatic Digestion: Purified histones are subjected to controlled enzymatic digestion (e.g., trypsin, GluC, or ArgC) to generate PTM-retaining peptides suitable for downstream analysis.
3. Peptide Enrichment: Modified peptides are enriched using targeted strategies such as antibody-based affinity enrichment or chemical derivatization, depending on the PTM type.
4. LC-MS/MS Analysis: Enriched peptides are analyzed on high-resolution LC-MS/MS systems to acquire spectra with site-specific modification information.
5. Data Processing: Raw MS data are processed using specialized search engines and spectral libraries to identify and localize PTMs on histone peptides.
6. Bioinformatics Analysis: Identified modifications are annotated and quantified. Functional insights are derived through motif analysis, clustering, and pathway enrichment to reveal biological relevance.
Figure 2. Workflow for Histone PTM Identification Service
Service Advantages
☑️Advanced Analysis Platform: MtoZ Biolabs established an advanced Histone PTM Identification Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
☑️One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
☑️High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all histone PTM identification data, providing clients with a comprehensive data report.
☑️One-Stop Service: We provide end-to-end workflow covering sample preparation, LC-MS/MS analysis, and data interpretation, saving your time and effort.
Applications
1. Epigenetic Regulation Studies
Histone PTMs play central roles in chromatin remodeling and gene expression regulation. Our service provides site-specific modification profiles to support studies on transcriptional activation, repression, and histone code interpretation.
2. Disease Mechanism Research
Aberrant histone modifications are key features of cancer and other diseases. By profiling differential PTM patterns, researchers can uncover potential biomarkers, oncogenic mechanisms, and therapeutic targets related to epigenetic dysregulation.
3. Drug Development
Epigenetic drugs (e.g., HDAC and methyltransferase inhibitors) target histone-modifying enzymes. Our platform enables quantitative monitoring of histone PTM changes in response to treatment, supporting drug screening, efficacy evaluation, and mechanism-of-action studies.
4. Developmental Biology
Histone PTMs guide lineage specification, stem cell differentiation, and reprogramming. This service helps characterize the dynamic histone modification landscape during developmental processes or induced cell fate transitions.
Sample Submission Suggestions
*Note: If you have specific requirements or need guidance on sample preparation, please do not hesitate to contact us.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Histone Ubiquitination Analysis
Histone PTM Data Analysis Service
Histone PTMs Quantitative Analysis Service
Histone Post-Translational Modification (PTM) Proteomics Service
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