Histone Post-Translational Modification (PTM) Proteomics Service

Histone post-translational modification (PTM) proteomics is an advanced field that systematically characterizes histone chemical modifications, their sites, and abundance using high-throughput techniques. As a central mechanism in epigenetic regulation, histone PTMs dynamically alter chromatin structure or recruit effector proteins to regulate crucial biological processes such as gene transcription, DNA repair, and cell fate determination. However, traditional antibody-dependent detection methods face challenges in throughput and limited coverage, making them unsuitable for the demands of complex biological studies.

 



Liu, R. Q. et al. MedComm. 2023.

Figure 1. Comprehensive Mapping of Histone Post-Translational Modifications (PTMs)

 

MtoZ Biolabs offers a one-stop Histone Post-Translational Modification (PTM) Proteomics Service that utilizes high-precision mass spectrometry platforms such as Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos, combined with Nano-LC chromatography separation technology. You only need to define your experimental goals and provide samples, and we will manage the entire workflow, from histone isolation and purification to mass spectrometry detection and data analysis. Our Histone Post-Translational Modification (PTM) Proteomics Service guarantees the precise identification of modification types and sites, such as methylation, acetylation, and phosphorylation, while providing both absolute and relative quantification results.

 

Services at MtoZ Biolabs

Accurate analysis of histone post-translational modifications relies on efficient purification systems and high-sensitivity detection technologies. Histone Post-Translational Modification (PTM) Proteomics Service at MtoZ Biolabs adopts a comprehensive approach of "nuclear-level histone purification - multi-dimensional enzymatic digestion - mass spectrometry integration", ensuring reliable and thorough PTM analysis.

·  High-Purity Histone Isolation: Optimized based on Extraction, Purification and Analysis of Histone (Nature Protocols), we isolate nuclei by cytoplasmic disruption and low-speed centrifugation, followed by impurity removal with high-salt buffers. Core histones (H2A, H2B, H3, H4) are then purified using HPLC, ensuring >95% purity.

·  Multi-Dimensional Enzymatic Digestion: We utilize a combination of Trypsin, Lys-C, and Glu-C for enzymatic digestion, overcoming the peptide length limitations of single-enzyme digestion and significantly improving the ionization efficiency of hydrophobic modified peptides.

·  High-Resolution Mass Spectrometry Analysis: With Q Exactive HF and Orbitrap Fusion mass spectrometers, paired with Nano-LC chromatography, we achieve deep coverage of modified peptides.

·  Dynamic Quantitative Analysis: We employ Label-free quantification or TMT-based quantification, enabling both site-specific and global PTM comparisons.

 

Analysis Workflow

To ensure a comprehensive and systematic analysis of histone modification patterns, MtoZ Biolabs’ Histone Post-Translational Modification (PTM) Proteomics Service follows a scientifically rigorous experimental workflow, ensuring high-quality and reliable results at every step:

1. Sample Preparation: Cell or tissue sample lysis, histone extraction, and protein quantification.

2. Histone Enrichment: Utilization of specific antibodies or chemical enrichment methods to isolate modified histones.

3. Enzymatic Digestion: Histone digestion with enzymes such as ArgC, Trypsin, and Glu-C, preserving modification site information.

4. Mass Spectrometry Analysis: Detection of histone PTMs using Nano-LC-MS/MS, generating high-precision mass spectrometry data.

5. Data Analysis: PTM identification and quantification using professional databases such as Uniprot and MaxQuant, followed by modification site functional network construction.

 

Why Choose MtoZ Biolabs?

1. Comprehensive Modification Coverage

Our Histone Post-Translational Modification (PTM) Proteomics Service supports a wide range of PTM analyses, including acetylation, methylation, and phosphorylation, enabling a complete capture of histone modification information.

 

2. Expert Bioinformatics Support

Our multi-layer bioinformatics analysis pipeline provides modification site prediction, functional annotation, and pathway analysis, offering in-depth biological insights.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data, providing clients with a comprehensive data report.

 

4. Customized Service

Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Suggestions

To ensure high precision and stability in mass spectrometry analysis, Histone Post-Translational Modification (PTM) Proteomics Service maintains strict quality standards for sample submission, ensuring data reliability and reproducibility.

 

Sample Type	Minimum Requirement	Handling Recommendations
Animal Tissue	2 g (wet weight)	Snap-freeze in liquid nitrogen and store on dry ice; avoid crosslinking agents
Plant Tissue	400 mg (wet weight)	Remove fibrous regions, freeze in liquid nitrogen
Whole Blood	2 mL	Anticoagulated with EDTA, avoid repeated freeze-thaw cycles
Plasma/Serum	2 mL	Snap-freeze after separation, add protease inhibitors
Urine	10 mL	Centrifuge to remove precipitates, store at low temperature
Cell Samples	1 × 10⁸ cells	Wash with PBS, freeze in liquid nitrogen; for suspension cells, centrifuge to remove medium
Yeast/Microorganisms	400 mg (dry weight)	Collect by centrifugation, freeze in liquid nitrogen
Protein Samples	2-5 mg (total amount)	Extract with standard lysis buffer, avoid strong denaturants

 

For special sample types or customized processing requirements, please contact our technical support team for tailored solutions.

 

Applications

MtoZ Biolabs’ Histone Post-Translational Modification (PTM) Proteomics Service provides robust support for life sciences and medical research, with applications spanning epigenetics, disease research, and drug discovery.

·  Epigenetics Research: Investigating the role of histone modifications in gene regulation.

·  Disease Mechanism Studies: Exploring how histone PTMs contribute to cancer, autoimmune diseases, and neurodegenerative disorders.

·  Drug Target Discovery: Identifying therapeutic targets associated with epigenetic regulation.

·  Stem Cell and Developmental Biology: Examining the impact of histone modifications on cell fate determination.

·  Chromatin State Profiling: Mapping histone modifications in different physiological and pathological conditions.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs leverages cutting-edge mass spectrometry technology, extensive proteomics expertise, and advanced bioinformatics analysis to deliver high-quality Histone Post-Translational Modification (PTM) Proteomics solutions. Whether for basic research or translational applications, we provide precise, efficient, and comprehensive analytical services to advance your scientific breakthroughs.