Histone Phosphorylation Analysis Service

Histone phosphorylation, a dynamic post-translational modification, regulates chromatin structure and gene activity by introducing phosphate groups to specific residues. Histone phosphorylation is critical for DNA repair, transcriptional regulation, and disease progression. MtoZ Biolabs offers advanced histone phosphorylation analysis service, combining antibody specificity and mass spectrometry precision to deliver actionable insights for academia and biotech research.    

 

Analysis Workflow  

1. Sample Submission: We accepts cells, tissues, or purified histones (minimal sample loss protocols).   

2. Modification Enrichment: Optimize sensitivity with optional immunoaffinity purification.  

3. Detection & Quantitation: Histone phosphorylation can be detected by antibody-based assays (WB/ELISA) or mass spectrometry according to samples’ type.  

4. Detailed Reporting: Following comprehensive data analysis, we offer phosphorylation levels, statistical analysis, and actionable insights.  

 



Robusti, G. et al. Clin Epigenetics. 2022.

Figure 1. The Workflow of Histone Phosphorylation Analysis

 

Why Choose MtoZ Biolabs?  

✅ Unmatched Precision: We can detect modifications at ultralow abundance (0.1% coverage) with rigorously validated antibodies.  

✅ Transparent Pricing: One-time charge with no hidden fees.  

✅ Expert Support: MtoZ Biolabs collaborates with specialized histone biologists for end-to-end guidance, from experimental design to data interpretation.  

✅ High-Resolution: High-resolution Thermo Fisher Q Exactive™ platform with accuracy <3 ppm.

 

Case Study

1. Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting

 



Zhao, Y. et al. Mol Cell. 2023.

Figure 2. Histone H3S28 is phosphorylated in response to low glucose

 

2. Specific phosphorylation of histone demethylase KDM3A determines target gene expression in response to heat shock

 



Cheng, MB. et al. PLoS Biol. 2014.

Figure 3. KDM3A is phosphorylated at S264 by MSK1 under HS conditions

 

Applications  

1. Disease Mechanism Studies: Linking aberrant phosphorylation to oncogenic pathways.  

2. Drug Development Validation: Assessing kinase inhibitor efficacy by monitoring site-specific modulation.  

3. Chromatin Dynamics Research: Investigating phosphorylation-driven structural changes during DNA repair or transcriptional activation.  

 

Deliverables  

1. Raw Data: Mass spectrometry files or antibody assay images.  

2. Visualized Reports: Heatmaps, dynamic curves, and pathway enrichment analysis. 

3. Publication-Ready Figures: Templates formatted for high-impact journals.  

  

MtoZ Biolabs’ histone phosphorylation analysis service empowers researchers worldwide with accurate, efficient, and reproducible epigenetic insights. Leveraging high-resolution mass spectrometry and 40⁺ validated antibodies, we decode dynamic modifications—from classic markers like γH2AX to novel sites—delivering rapid, standardized results to uncover phosphorylation’s role in DNA repair, transcription, and disease. Whether validating drug targets or exploring chromatin dynamics, our dual-technology platform (antibody + mass spectrometry) and expert team ensure data clarity, traceability, and scientific impact.