Histone Phosphorylation Analysis Service
Histone phosphorylation, a dynamic post-translational modification, regulates chromatin structure and gene activity by introducing phosphate groups to specific residues. Histone phosphorylation is critical for DNA repair, transcriptional regulation, and disease progression. MtoZ Biolabs offers an advanced histone phosphorylation analysis service, combining antibody specificity and mass spectrometry precision to deliver actionable insights for academia and biotech research.
Services at MtoZ Biolabs
1. Target Histone Phosphorylation Analysis
MtoZ Biolabs provides Target Histone Phosphorylation Analysis focused on the precise detection and quantification of phosphorylation modifications on specific histones. By combining phospho-specific antibody enrichment with high-resolution LC-MS/MS analysis, we accurately localize phosphorylation sites and evaluate dynamic changes. Multiple fragmentation modes are employed during MS analysis, including higher-energy collisional dissociation (HCD), lectron transfer dissociation (ETD), and electron transfer/higher-energy collisional dissociation (EThcD), ensuring accurate site identification even in complex samples with multiple coexisting modifications. This service is suitable for drug target validation, chromatin remodeling studies, and functional investigations of histone phosphorylation in DNA repair and transcriptional regulation.
2. Histone Phosphorylation Proteomics Analysis
MtoZ Biolabs' Histone Phosphorylation Proteomics Analysis utilizes a high-sensitivity mass spectrometry platform and multi-antibody enrichment strategies to systematically characterize histone phosphorylation profiles across various cells or tissues. The analytical workflow integrates multiple fragmentation modes, including HCD, ETD, and EThcD, to achieve high-confidence identification and quantification of phosphorylation sites. Combined with bioinformatics analysis, this service reveals the regulatory roles of histone phosphorylation in epigenetic modulation, signaling pathways, and disease progression, providing comprehensive technical support from modification discovery to mechanistic interpretation.
Analysis Workflow
1. Sample Submission: We accepts cells, tissues, or purified histones (minimal sample loss protocols).
2. Modification Enrichment: Optimize sensitivity with optional immunoaffinity purification.
3. Detection and Quantitation: Histone phosphorylation can be detected by antibody-based assays (WB/ELISA) or mass spectrometry according to sample' type.
4. Detailed Reporting: Following comprehensive data analysis, we offer phosphorylation levels, statistical analysis, and actionable insights.
Robusti, G. et al. Clin Epigenetics. 2022.
Figure 1. The Workflow of Histone Phosphorylation Analysis
Why Choose MtoZ Biolabs?
✅ Unmatched Precision: We can detect modifications at ultralow abundance (0.1% coverage) with rigorously validated antibodies.
✅ Transparent Pricing: One-time charge with no hidden fees.
✅ Expert Support: MtoZ Biolabs collaborates with specialized histone biologists for end-to-end guidance, from experimental design to data interpretation.
✅ High-Resolution: High-resolution Thermo Fisher Q Exactive™ platform with accuracy <3 ppm.
Case Study
1. Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting
Zhao, Y. et al. Mol Cell. 2023.
Figure 2. Histone H3S28 is phosphorylated in response to low glucose
2. Specific phosphorylation of histone demethylase KDM3A determines target gene expression in response to heat shock
Cheng, MB. et al. PLoS Biol. 2014.
Figure 3. KDM3A is phosphorylated at S264 by MSK1 under HS conditions
Applications
1. Disease Mechanism Studies: Linking aberrant phosphorylation to oncogenic pathways.
2. Drug Development Validation: Assessing kinase inhibitor efficacy by monitoring site-specific modulation.
3. Chromatin Dynamics Research: Investigating phosphorylation-driven structural changes during DNA repair or transcriptional activation.
Deliverables
1. Raw Data: Mass spectrometry files or antibody assay images.
2. Visualized Reports: Heatmaps, dynamic curves, and pathway enrichment analysis.
3. Publication-Ready Figures: Templates formatted for high-impact journals.
MtoZ Biolabs' histone phosphorylation analysis service provides researchers worldwide with precise, efficient, and reproducible insights into epigenetic regulation. By integrating high-resolution mass spectrometry with a validated panel of over 40 phosphorylation-specific antibodies, we accurately profile dynamic phosphorylation events on histones, revealing their roles in DNA repair, transcriptional regulation, and disease progression. Our dual-technology platform, combining antibody-based enrichment and advanced MS detection, ensures high sensitivity, quantitative reliability, and site-specific resolution. Contact us to obtain customized services and technical support.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Histone Post-Translational Modification Analysis Service
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