Mass Spectrometry-Based Protein Sequencing Service
Protein sequencing is a core technique for decoding the amino acid order of proteins. It not only helps confirm the molecular identity of proteins but also reveals their structural features and functional domains. Sequencing provides complete protein sequence information, identifies potential variants and modifications, and offers reliable support for studies on protein function, antibody development, and biopharmaceutical quality control. With the advancement of high-resolution mass spectrometry, mass spectrometry-based protein sequencing has become the mainstream approach. It enables rapid and precise sequence analysis in complex samples and is applicable to a wide range of research needs, from peptides to full-length proteins.
Leveraging state-of-the-art high-resolution mass spectrometry platforms and a standardized analytical workflow, MtoZ Biolabs offers Mass Spectrometry-Based Protein Sequencing Service covering peptide sequencing, full-length protein sequencing, antibody sequencing, and N/C-terminal sequencing, providing robust support for life science research, antibody drug development, and biopharmaceutical quality control.
Services at MtoZ Biolabs
The mass spectrometry-based protein sequencing service at MtoZ Biolabs is primarily based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). By enzymatically digesting, separating, and fragmenting proteins, we generate peptide coverage across the entire sequence, which is then interpreted through database searches or De Novo sequencing algorithms to reconstruct the amino acid sequence. Our service portfolio includes:
Analysis Workflow
1. Sample Preparation: Purification or buffer exchange tailored to sample type.
2. Enzymatic Digestion: Multi-enzyme digestion (e.g., trypsin, Glu-C) to enhance coverage.
3. Liquid Chromatography Separation: Nano-LC separation to reduce complexity.
4. Mass Spectrometry Analysis: High-resolution Orbitrap or Q-TOF MS/MS analysis.
5. Sequence Reconstruction: Database search or De Novo sequencing.
6. Data Integration and Reporting: Complete report with sequence and experimental details.
Service Advantages
1. High Resolution and Sensitivity
Equipped with Orbitrap and Q Exactive HF platforms, MtoZ Biolabs ensures accurate detection of low-abundance proteins and modifications with excellent reproducibility.
2. Comprehensive Coverage and Versatile Methods
From peptide-level to full-length sequencing, database-dependent or De Novo approaches, we provide flexible solutions suitable for a wide range of research applications.
3. Strong Capability for Modification and Mutation Detection
By combining top-down and bottom-up strategies, our mass spectrometry-based protein sequencing service not only delivers sequence information but also reveals phosphorylation, glycosylation, acetylation, and potential mutation sites.
4. Experienced Team and Standardized Workflow
With extensive expertise in protein sequencing and mass spectrometry analysis, our team ensures high-quality results through standardized protocols and rigorous quality control.
5. One-Stop Service Platform
From sample reception and preparation to sequencing and bioinformatics interpretation, all processes are completed within one platform, minimizing inter-lab variability and improving efficiency.
Applications
✅Basic Research: Characterizing novel proteins and validating gene expression products.
✅Antibody Drug Development: Full-length antibody sequencing and CDR determination for drug discovery and patent filing.
✅Biopharmaceutical Quality Control: Verifying recombinant proteins and peptide drugs.
✅Disease Research: Detecting mutations and post-translational modifications relevant to disease mechanisms.
✅Biomarker Discovery: Identifying protein sequences for diagnostics and prognostics.
FAQs
Q1: Can you sequence unknown proteins?
A1: Yes. Using De Novo sequencing combined with mass spectrometry, we can deduce sequences even without a reference database.
Q2: Can protein modifications be detected?
A2: Yes. Our mass spectrometry-based protein sequencing service can identify common post-translational modifications alongside sequencing.
Q3: What is the typical turnaround time?
A3: Standard projects are generally completed within several weeks, depending on sample complexity and sequencing type.
Sample Submission Suggestions
1. Serum/Plasma: ≥200 µL per sample
2. Purified Protein: ≥20 µg with ≥80% purity
3. Tissue Samples: ≥50 mg, stored at low temperature
4. Cell Samples: ≥1×10⁶ cells
5. Transport: Ship on dry ice; avoid repeated freeze-thaw cycles
Please provide background information (e.g., species origin, expected molecular weight, presence of tags) to optimize analysis.
Deliverables
1. Project report
2. Experimental workflow and MS parameters
3. Raw MS data (RAW/mzML format)
4. Protein sequence results
5. Detected post-translational modifications and mutation sites (if applicable)
6. Data visualization (spectra, coverage maps, etc.)
MtoZ Biolabs provides mass spectrometry-based protein sequencing service with advanced platforms and professional expertise. Whether for basic research, antibody drug development, or biopharmaceutical quality control, we deliver reliable sequencing solutions to support your projects.
Contact us today for more information and let us help accelerate your research.
How to order?
