Mass Spectrometry Based Antibody Sequencing Service

Antibodies are pivotal to the immune system, endowed with unique structures that provide high specificity and versatility. With biotechnological advancements, their use in disease diagnosis and therapeutic interventions has expanded significantly. In pharmaceutical development, precise characterization of antibody sequences and structures is paramount for ensuring therapeutic efficacy and safety. Mass Spectrometry (MS), renowned for its accuracy and throughput, is now a critical tool for antibody analysis. MS not only delivers complete antibody sequencing but also exposes detailed post-translational modifications, proving vital in research and development efforts.

At MtoZ Biolabs, leveraging the Orbitrap Fusion Lumos — the mass spectrometer with the highest resolution available — combined with our extensive experience in bioinformatics, we have developed a cutting-edge antibody sequencing platform. This platform performs fast and precise primary structural analyses of antibodies, accommodating various subtypes (e.g., IgG, IgM) and forms (e.g., fluorescently labeled, immobilized, cross-species).

Analysis Workflow

1. Sample Preparation

Antibody samples undergo purification and digestion to prepare for MS analysis.

2. Mass Spectrometry Analysis

High-resolution spectrometry is employed to accurately measure antibody peptides.

3. Data Processing

Advanced algorithms and database matching refine MS data, ensuring precise sequence identification.

4. Results Verification

Bioinformatics tools validate and elucidate the findings, confirming their accuracy and relevance.

Figure 1. Analysis Workflow of Antibody Sequencing

Service at MtoZ Biolabs

·  CDR sequencing

·  Comprehensive amino acid sequencing

·  De novo antibody sequencing

·  Variable region sequencing of light and heavy chains

·  Comparative sequencing of monoclonal antibodies and biosimilars

Service Advantages

Full Sequence Coverage: From the N-terminus to the C-terminus, our high-resolution MS provides exhaustive sequence analysis.

Versatility: Our techniques are applicable to a wide range of antibody types and species, enhancing utility across different scientific fields.

High-throughput Capability: Our platform efficiently handles both individual and batch analyses of antibodies.

Applications

Pharmaceutical Development

Diagnostic Testing

Biosimilarity Evaluations

Immunology Research

Sample Results

Direct Mass Spectrometry-Based Detection and Antibody Sequencing of Monoclonal Gammopathy of Undetermined Significance from Patient Serum

Peng, W. W. et al. bioRxiv. 2023.

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Antibody Sequences

4. Mass Spectrometry Images

5. Raw Data

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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