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    Protein C-Terminal Sequencing Service

      Protein C-terminal sequencing is essential for analyzing protein integrity and serves as a key gateway to understanding their biological functions and regulatory mechanisms. Although typically brief, the C-terminal region carries diverse biological roles, including serving as localization signals, binding sites, and regulatory areas. These functionalities are often linked to its structure and specific C-terminal modifications such as phosphorylation, glycosylation, or other types of modifications. Thus, precise C-terminal sequence information not only aids in comprehending the biological roles of proteins but is also pivotal in researching disease-related functional anomalies and their regulatory mechanisms.


      At MtoZ Biolabs, our C-terminal sequencing service employs High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), combined with cutting-edge sample processing methods and algorithms, to efficiently isolate and analyze proteins across various samples. Utilizing advanced Collision-Induced Dissociation (CID) technology, we ensure the acquisition of clear and precise sequence data from each protein sample.



      Figure1. Protein C-Terminal Sequencing Workflow



      1. Protein Processing and Maturation Analysis

      Ascertain the processing state of the protein C-terminus to elucidate its biological functions.

      2. Protein Expression Verification

      Confirm the complete expression and integrity of bioengineered proteins throughout their expression process.

      3. Post-Translational Modification Analysis

      Identify and characterize modifications at the protein C-terminus, providing essential insights into protein functionalities.

      4. Pharmaceutical Development Support

      Supply vital protein sequence information to aid in drug design and development processes.


      Sample Submission Requirements

      1. Sample Types

      We can process liquid samples, gel bands, or gel spots.

      2. Protein Amount

      A minimum of 5-10 μg is recommended for optimal analysis results.

      3. Sample Purity

      High purity samples yield more accurate outcomes. For samples under 10 μg, minimize the use of excessive detergents and salts to maintain quality.


      Service Advantages

      1. Comprehensive Coverage: Our technology ensures 100% sequence coverage of the target protein’s C-terminus.

      2. Efficiency: Simultaneous sequencing of both the N-terminus and C-terminus drastically reduces the experimental timeline.

      3. Sensitivity: Our systems can detect extremely low-abundance proteins, guaranteeing the accuracy and reliability of our results.


      MtoZ Biolabs is dedicated to delivering high-quality protein C-terminus sequencing services, complemented by professional technical support and customer service. Each research project is guaranteed to receive the highest quality analysis. If you require assistance or have inquiries regarding protein C-terminus sequencing, please contact us. Our expert team is ready to offer personalized solutions tailored to your specific needs.


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