Integrated Immunopeptidomics Analysis Service
The immune peptidome refers to a collection of peptides related to the immune system, especially those that bind to immune receptors. The study of the immune peptidome aims to gain a deeper understanding of how the immune system recognizes and presents antigens through peptides, thereby triggering immune responses. This service utilizes advanced analytical methods to extract and enrich peptides associated with the immune system from biological samples, followed by qualitative and quantitative analysis. Through in-depth analysis of peptide sequences, abundance, modification status, and more, it can reveal immune recognition, antigen presentation, and immune response mechanisms.
The integrated immunopeptidomics analysis service is widely applied in tumor immunology research, vaccine development, immune escape mechanism studies, and disease biomarker discovery. Immune peptidomics analysis provides critical data support for immunotherapy, personalized vaccine design, and immune target validation. Additionally, it helps uncover complex immune mechanisms such as tumor immune escape and immune tolerance, contributing to the advancement of precision immunotherapy.
Chong, C. et al. Nature Biotechnology, 2022.
Figure 1. The HLA-I Processing and Presentation Pathway, Along with the Processes that Can Potentially Generate Noncanonical Peptides.
Services at MtoZ Biolabs
Based on advanced technologies and analytical platforms, MtoZ Biolabs offers the integrated immunopeptidomics analysis service focusing on analyzing endogenous peptides that bind to immune receptors. This service extracts and enriches immune-related peptides from biological samples and performs high-throughput analysis to obtain peptide sequence information, abundance data, post-translational modification status, and more. Customers receive high-quality peptide data that supports applications such as immune epitope prediction, antigen discovery, immune escape mechanism research, and vaccine design. We provide the following services:
1. Peptidomics-Based Antigen Discovery and Prediction Service
By using high-resolution mass spectrometry, this service analyzes peptides from the immune system to discover potential immune epitopes and novel antigens, providing data support for immunotherapy and vaccine design.
2. Immune Peptide Library Screening Service
Using immunoaffinity purification techniques, we construct diverse peptide libraries and perform high-throughput screening to identify peptides that bind with immune receptors.
3. Immunopeptide Affinity Screening Service
By utilizing an efficient affinity screening platform, we select peptides with high binding affinity to immune receptors, helping assess their immunogenicity and providing precise data for immune target discovery.
4. HLA Peptidomics Analysis Service
This analysis focuses on the interaction between HLA molecules and peptides, revealing the characteristics of HLA peptide presentation and providing data support for immune recognition and cancer immunotherapy.
5. Immune Peptide Mass Spectrometry Analysis Service
Based on mass spectrometry platforms, we provide qualitative and quantitative analysis of immune-related peptides, revealing peptide sequences, abundance, and modification states, offering crucial data for immune response mechanisms and antigen presentation studies.
6. MHC-binding Peptides Identification Service
Using immunoaffinity purification techniques combined with high-resolution mass spectrometry, we identify peptides binding with MHC molecules, supporting immune monitoring and cancer immunotherapy target discovery.
Analysis Workflow
1. Sample Preparation
First, immune system-related peptides are extracted and purified to ensure high purity and specificity of the samples for subsequent analysis.
2. Peptide Enrichment and Screening
Using immunoaffinity purification techniques, peptides binding with immune receptors are enriched from biological samples, ensuring efficient screening and accurate acquisition of target peptides.
3. Mass Spectrometry Analysis (LC-MS/MS)
The enriched peptides are separated, identified, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS), providing peptide sequence information, abundance distribution, and modification states.
4. Data Analysis and Immune Function Annotation
Combining mass spectrometry data, the peptides are analyzed for immunogenicity, potential immune epitope prediction, and annotation of immune functions such as T-cell epitopes.
5. Result Interpretation and Report Generation
Based on the analysis results, a detailed report is generated, providing qualitative and quantitative data on peptides, immunogenicity assessments, and other relevant information to support further research and immune therapy strategies.
Service Advantages
1. Precise Mass Spectrometry Analysis
Based on high-resolution mass spectrometry platforms (such as LC-MS/MS), we provide precise peptide sequence information, abundance data, and post-translational modification states, ensuring high-quality and accurate results.
2. Multidimensional Immune Function Analysis
We offer not only peptide quantification data but also integrate immunogenicity prediction, T-cell epitope analysis, and other functional annotations to deeply explore the role of key peptides in the immune system.
3. Customized Service Solutions
Tailored analysis plans are designed based on specific customer needs, supporting the screening and analysis of various immune receptors to meet diverse research requirements.
4. One-Stop Comprehensive Service
We offer a complete service process from sample preparation, peptide screening, mass spectrometry analysis, to data interpretation, simplifying experimental procedures and enhancing research efficiency.
Applications
1. Tumor Immunity Research
By analyzing the immune peptide repertoire in tumor tissues, we can identify and predict potential neoantigens, providing valuable data for tumor immunotherapy target discovery and supporting the development of immune-based therapies.
2. Vaccine Development
The Integrated Immunopeptidomics Analysis Service is used to screen peptides that bind to immune receptors, assisting in the design of more immunogenic vaccine epitopes, thereby improving the vaccine's immunological effectiveness and targeting specificity.
3. Immunomonitoring
Combining immune peptide analysis with immunogenicity evaluation, this service provides precise data for immunomonitoring, advancing the field of autoimmune research and enabling better monitoring of immune responses.
4. Immune Evasion Mechanism Research
The Integrated Immunopeptidomics Analysis Service can be applied to study the role of immune peptides in immune evasion by tumors or pathogens, revealing key peptides involved in immune escape, and providing data support for the development of novel immune therapy strategies.
FAQ
Q1: Can this Service Analyze Post-Translationally Modified Peptides?
A1: Yes, the Integrated Immunopeptidomics Analysis Service can identify and analyze a variety of post-translationally modified peptides, including phosphorylation, acetylation, methylation, and others. If you require the analysis of post-translational modifications, please contact us in advance, and we will customize the experimental plan according to your needs.
Q2: What Kind of Data Will Clients Receive Upon Completion of the Service?
A2: Clients will receive comprehensive qualitative and quantitative data for the peptides, including peptide sequence information, abundance distribution, modification status, and immunogenicity analysis results. Additionally, we provide T-cell epitope prediction and immune epitope analysis, helping clients further understand the key peptides involved in immune responses.
Deliverables
1. Comprehensive Experimental Details
2. Identified Peptides and Quantitative Data
3. Data Analysis, Preprocessing, and Estimation
4. Bioinformatics Analysis
5. Raw Data Files
Related Services
Peptidomics-Based Antigen Discovery and Prediction Service
Immune Peptide Library Screening Service
Immunopeptide Affinity Screening Service
HLA Peptidomics Analysis Service
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