Immunopeptide Affinity Screening Service

Immunopeptide affinity screening analysis is a method that utilizes high-throughput affinity screening technology to identify peptides with high binding affinity to specific immune receptors, such as antibodies or T-cell receptors. This service constructs a diverse peptide library and employs methods such as phage display, yeast display, or in vitro selection. Through antibody or receptor capture, candidate peptides are enriched, and using advanced affinity screening platforms, peptides that bind to the target immune receptor are selected.

 

The immunopeptide affinity screening service is widely applied in fields such as antibody drug development, vaccine epitope screening, tumor immune target discovery, and immunotherapy target research. By efficiently screening peptides that bind to immune receptors, researchers can discover new immune epitopes, evaluate the immunogenicity of peptides, and provide data support for the development of immune targets. This technology can also be used for protein-protein interaction studies, epitope prediction, and immune monitoring, advancing the development of personalized immunotherapy and precision vaccine design.

 

Services at MtoZ Biolabs

Based on advanced affinity screening platforms, MtoZ Biolabs offers the immunopeptide affinity screening service which focuses on analyzing the binding affinity between immune receptors and peptides. This service combines affinity screening technology with high-sensitivity mass spectrometry, enabling the efficient screening and identification of peptides with high binding affinity to target receptors. Clients will receive data on peptide binding affinity, qualitative sequence information, modification states, and more. The services offered by MtoZ Biolabs include, but are not limited to, the following:

 

1. Peptide Library Screening

By constructing a diverse peptide library and using immune receptors for high-throughput screening, this method rapidly identifies peptides with high binding affinity to immune receptors, making it suitable for various immune research needs.

 

2. Yeast Display

Using yeast display technology, peptides are presented on the surface of yeast cells and screened for binding to immune receptors. This method is highly efficient and suitable for antibody drug development and vaccine design.

 

3. In Vitro Selection

In vitro selection technologies, such as RNA display or ribosome display, are used to screen for peptides from the peptide library that bind strongly to immune receptors. This approach improves the precision of screening, providing a more efficient method for immune peptide screening.

 

4. Affinity Quantification

Screened peptides undergo affinity quantification, providing scientific data to support further immune research, vaccine development, and target validation.

 

5. Peptide-Receptor Binding Validation

Experimental validation is performed to confirm the binding characteristics of peptides with immune receptors, ensuring that the selected peptides have biological activity and potential as targets for immunotherapy or vaccine design.

 

Analysis Workflow

1. Sample Preparation

Provide the peptide library samples for screening, and extract target peptides to ensure their purity and specificity.

 

2. Peptide Library Screening and Affinity Measurement

Utilize high-throughput screening technology to rapidly screen peptides in the library and measure the binding affinity between the selected peptides and immune receptors, providing fundamental data for subsequent functional analysis.

 

3. Peptide Identification and Sequence Analysis

Use LC-MS/MS technology to identify the sequences of the screened peptides, determine their source, and analyze their modification states, abundance, and other properties.

 

4. Data Analysis and Result Interpretation

Combine mass spectrometry data to assess immunogenicity, select peptides with immune activity, and provide precise immune target and antigen information for further research.

 

Service Advantages

1. Efficient Screening Capability

Using high-throughput screening technology, a large number of peptides from various samples can be screened in a short period, significantly improving the efficiency of immune peptide screening and the range of data collection.

 

2. Precise Affinity Analysis

By utilizing advanced affinity screening technologies, we provide accurate binding affinity data between peptides and immune receptors, ensuring the reliability of the screening results.

 

3. Customized Service Plans

Based on the specific needs of clients, we offer personalized immune peptide screening plans, ensuring the precise achievement of research goals and supporting the screening of different immune receptors.

 

4. One-Stop Service Process

We offer a complete service process, from sample preparation, peptide screening, affinity quantification, to data analysis, simplifying experimental operations and enhancing research efficiency.

 

Applications

1. Tumor Immunotherapy Target Discovery

By screening immune peptides from tumor tissues, potential new tumor antigens are identified, providing precise data support for tumor immunotherapy target discovery.

 

2. Vaccine Epitope Optimization

The immunopeptide affinity screening service helps optimize vaccine epitope design, enhancing the immune response and specificity of the vaccine.

 

3. Immune Escape Research

By screening peptides that bind to immune receptors, this service explores how tumors or pathogens escape immune surveillance through specific peptides, helping develop new therapeutic strategies related to immune escape mechanisms.

 

4. Immune Response Research

The immunopeptide affinity screening service can be used to analyze peptides binding to immune receptors, revealing immune response mechanisms and supporting research in immunotherapy, personalized immune treatment, and immune monitoring.