Amidation Proteomics Service
Amidation, the enzymatic transfer of an amide group (–NH₂) to a peptide’s C-terminus, is an essential post-translational modification (PTM) in the regulation of protein function, stability, and interactions. It occurs predominantly on the C-terminal of peptides and proteins, and it has a significant impact on protein activity, signaling, and the overall proteome. This modification is often associated with neuropeptides, hormones, and cytokines, as it influences protein secretion, receptor binding, and biological half-life. Amidation is catalyzed by enzymes known as peptidylglycine α-amidating monooxygenases (PAMs), which require oxygen and a cofactor to add the amide group.
Figure 1. The Amidation Reaction Catalyzed by PAM is Shown
Service at MtoZ Biolabs
MtoZ Biolabs offers a specialized Amidation Proteomics Service designed to meet the growing need for precise, quantitative, and site-specific analysis of amidated peptides. By combining advanced mass spectrometry, enriched sample preparation techniques, and comprehensive bioinformatics, we provide unparalleled insights into the role of amidation in cellular processes and disease mechanisms. Our service helps researchers uncover the biological significance of amidation in a wide range of applications, from neuropeptide analysis to drug target identification, offering an integrated solution from sample preparation to data interpretation. In addition to Amidation Proteomics, we also provide comprehensive analysis of other post-translational modifications (PTMs), including phosphorylation, glycosylation, acetylation, and more, enabling a holistic approach to protein modification research.
Figure 2. Workflow for Amidation Proteomics Service
Service Advantages
✔️Advanced Analysis Platforms: MtoZ Biolabs utilizes high-resolution mass spectrometry platforms for accurate, high-sensitivity analysis of amidated peptides, ensuring reliable data.
✔️Customizable Solutions: From sample preparation and peptide enrichment to quantitative analysis and bioinformatics interpretation, we offer a flexible, end-to-end service that is tailored to your specific research needs and experimental design.
✔️Experienced Team: Our scientists are experts in PTM analysis and proteomics. We provide personalized support and consultation throughout the entire project, ensuring the highest quality results.
✔️Comprehensive Results: Receive detailed, publication-ready reports that include raw data, identified amidated peptides, functional annotations, and quantitative analysis, providing a thorough understanding of your study results.
Applications
1. Neuropeptide and Hormone Regulation
Amidation plays a crucial role in the stability and activity of neuropeptides and hormones. Our service allows the precise identification and quantification of amidated peptides, such as neurotransmitters and endocrine hormones, to explore their biological functions and dysregulations in disease.
2. Disease Mechanism Studies
Amidation modifications have been implicated in various diseases, including neurodegenerative disorders, cancer, and inflammatory conditions. By comparing amidation levels across normal and diseased samples, our service helps uncover modification-driven regulatory mechanisms and identify potential biomarkers.
3. Drug Development and Target Identification
Amidation is a key modification in peptide-based therapeutics. Our service aids in the characterization of amidated drug targets, providing insights into the mechanism of action of peptide-based drugs, their stability, and efficacy.
4. Protein Interactions and Signaling Pathways
Amidated peptides are often involved in protein–protein interactions and signaling pathways. Our service can be used to map amidation-related signaling events, linking them to receptor activation, cellular responses, and disease progression.
Sample Submission Suggestions
Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice to ensure the stability of protein.
*Note: For any specific requirements or assistance with sample preparation, please feel free to contact us.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Post-translational Modifications (PTMs) Service
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