Acetylated Protein Analysis Service

Acetylation is a post-translational modification (PTM) where an acetyl group (CH3CO) is enzymatically added to specific amino acid residues, typically lysine (Lys). This modification is mediated by acetyltransferases (HATs) and can be reversed by deacetylases (HDACs). Acetylation can modify protein function, structure, and interactions, impacting various cellular physiological processes.

Zhang, H. et al. Front. Oncol. 2020.

Figure 1. Reversible Regulation of Lysine Acetylation

Dysregulation of protein acetylation has been implicated in various diseases, such as cancer, cardiovascular conditions, and neurodegenerative disorders. Understanding the role of acetylated proteins and their modification sites is vital for elucidating disease mechanisms, identifying reliable biomarkers, and developing innovative therapeutic strategies.

MtoZ Biolabs is dedicated to delivering high-quality Acetylated Protein Analysis Service, helping clients uncover the intricacies of protein acetylation and advancing life sciences research. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

Analysis Workflow

1. Sample Preparation

Extraction of total proteins from cells, tissues, or bodily fluids after sample lysis.

2. Digestion

Digestion of the extracted protein samples into peptides using trypsin.

3. Acetylation Enrichment

Using specific antibodies or affinity materials to enrich for acetylated peptides.

4. Liquid Chromatography Separation

Separation of the peptides by liquid chromatography.

5. Mass Spectrometry Detection

Detection and analysis of peptides using mass spectrometry to obtain data on acetylation.

6. Data Analysis

Analysis of mass spectrometry data with specialized software to identify acetylation sites and their abundance.

Service Advantages

1. High Sensitivity

Utilization of advanced mass spectrometry and optimized enrichment methods ensures sensitive detection.

2. High Specificity

Specific antibodies analysis provide accurate enrichment of acetylated lysine residues.

3. Comprehensive Analysis

Our detailed information on acetylation sites and abundance helps clients understand protein modifications.

4. Professional Technical Support

Our experienced team offers expert technical consultation and data interpretation.

5. Customized Service

We offer tailored analysis plans to meet diverse research needs.

Sample Submission Requirements

For detailed sample submission instructions, please consult our technical team.

Applications

1. Disease Research

Analyzing acetylated proteins in disease-related samples to elucidate molecular mechanisms underlying disease onset and progression.

2. Drug Development                                             

Identifying and validating drug targets and investigating the effects of drugs on protein acetylation.

3. Biomarker Discovery

Identifying disease-associated acetylation sites to develop new diagnostic and prognostic biomarkers.

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Acetylated Protein Analysis

4. Mass Spectrometry Images

5. Raw Data

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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