Yeast Glycosylation Analysis Service
Glycosylation is one of the most important post-translational modifications of proteins, playing a crucial role in various biological processes such as cell function, signal transduction, and immune response. With the advancement of biotechnology, the study of yeast glycosylation has become an essential direction for exploring protein structure and function, particularly in biopharmaceutical and disease research. As a key research target in the biopharmaceutical industry, yeast glycosylation not only affects yeast growth and development but also plays an indispensable role in recombinant protein production and vaccine development.
Yeast glycosylation analysis primarily depends on mass spectrometry (MS), liquid chromatography (LC) and specific glycosylation labeling methods. MtoZ Biolabs utilizes a advanced LC-MS/MS platform, equipped with high-resolution mass spectrometers like Orbitrap Fusion Lumos and Q Exactive HF, enabling precise detection and quantitative analysis of glycosylation modifications. We also incorporate glycosylation-specific enrichment methods, such as Lectin enrichment and HILIC enrichment, ensuring high-sensitivity detection of low-abundance glycosylation modifications, providing a comprehensive analysis of glycosylation products in yeast cells.
MtoZ Biolabs is committed to providing high-quality Yeast Glycosylation Analysis Service to global research institutions and pharmaceutical companies. With advanced mass spectrometry technology and meticulous experimental design, we offer accurate yeast glycosylation analysis to help our clients gain deep insights into the biological functions of glycosylation and provide solid technical support for new drug development and disease mechanism research. If you are interested in our service, feel free to contact us, and we will provide detailed consultations and personalized solutions based on your needs.
Analysis Workflow
1. Sample Preparation
Sample preparation is a key step in glycosylation analysis. First, proteins are extracted from yeast samples, and then digested with trypsin to produce peptides suitable for analysis. Next, glycosylated peptides are selectively enriched using glycosylation enrichment methods to ensure efficient capture of the glycosylation modifications.
2. Liquid Chromatography-Mass Spectrometry (LC-MS/MS) Analysis
Enriched glycosylated peptides are separated using a liquid chromatography system (such as nano-LC) and then analyzed using high-resolution mass spectrometers. The mass spectrometer efficiently identifies glycosylation modification sites in complex backgrounds and confirms the modification structures using MS/MS technology.
3. Data Analysis and Interpretation
Using professional data analysis software and glycosylation modification databases, we conduct both quantitative and qualitative analysis of the obtained mass spectrometry data. The data provided will include glycosylation modification sites, glycosylation types, abundance, and other detailed information to provide comprehensive support for our clients' follow-up research.
Service Advantages
1. Global Leading Technology Platform
Relying on advanced mass spectrometry technology platforms and high-resolution mass spectrometers such as Orbitrap Fusion Lumos and Q Exactive HF, MtoZ Biolabs delivers efficient and precise analysis of glycosylation modifications. We provide one-stop service from sample submission to data report delivery, ensuring each step is executed accurately for the highest-quality analytical results.
2. High Sensitivity and Precision
With advanced MS technology, MtoZ Biolabs ensures the accurate identification and quantification of low-abundance glycosylation modifications, achieving sub-ppm mass accuracy for highly precise and reliable results. Our platform has extremely high sensitivity, capturing even minute amounts of glycosylation modifications, offering reliable and precise data support.
3. Wide Application Areas
MtoZ Biolabs can analyze both N-linked and O-linked glycosylation modifications in yeast, and delve into the dynamic changes of glycosylation modifications. Whether it's studying the mechanisms of glycosylation in basic research or optimizing yeast glycosylation in industrial production, we provide professional support to meet the needs of various fields.
4. Customized Services
We offer flexible service plans tailored to clients' specific research objectives, allowing for adjustments to sample processing workflows, glycosylation enrichment methods, and analysis depth. Whether it's a detailed analysis of specific glycosylation modifications or joint analysis of glycosylation with other post-translational modifications, we provide customized solutions to ensure the analysis results meet our clients' specific needs.
5. Comprehensive Data Analysis
Our analysis goes beyond identifying glycosylation sites; it also includes quantitative analysis of modifications, structural analysis of glycan chains, and the role of glycosylation modifications in protein function. By combining various data analysis tools, we provide comprehensive data support, with reports detailing glycosylation modification sites, abundance changes, glycan structure, and their biological function relationships, ensuring clients gain valuable insights.
Applications
1. Yeast Glycosylation Research
MtoZ Biolabs' yeast glycosylation analysis service is suitable for studying glycosylation patterns in yeast cells and understanding how glycosylation modifications affect yeast growth, metabolism, stress responses, and other physiological processes.
2. Recombinant Protein Production
In biopharmaceutical processes, yeast is an important expression system. Glycosylation plays a critical role in recombinant protein production and function. Yeast glycosylation analysis helps pharmaceutical companies optimize production processes and ensure that the glycosylation modifications of products meet standards.
3. Disease Mechanism Research
Glycosylation modifications play important roles in the occurrence and development of various diseases. MtoZ Biolabs' yeast glycosylation analysis service can provide data support for studying the role of glycosylation in diseases such as cancer and diabetes.
4. Vaccine Development
Glycosylation is a key factor influencing antigen presentation and immune response. Glycosylation analysis in vaccine development can help optimize antigen glycosylation properties and improve vaccine efficacy.
Sample Submission Suggestions
1. Sample Types
Our service supports various types of yeast samples, including yeast cells, yeast culture media, fermentation products, etc.
2. Sample Quantity
Each project requires at least 5-10 mg of total yeast protein to ensure adequate enrichment and analysis. For customized analysis needs, please contact us for detailed information.
3. Sample Preparation
Samples should be stored under frozen conditions to avoid repeated freeze-thaw cycles. Before submission, samples should undergo necessary desalting and impurity removal steps to ensure the accuracy of the analysis results.
Deliverables
1. Glycosylation Modification Site Report
2. Quantitative Analysis Report
3. Data Visualization Charts
4. Functional Annotation and Pathway Analysis
5. Raw Mass Spectrometry Data
6. Personalized Report and Analysis Content
MtoZ Biolabs has an advanced mass spectrometry technology platform and extensive experience in glycosylation analysis. We are committed to providing high-quality yeast glycosylation analysis service to our clients. If you are interested in our services or have any technical questions, feel free to contact us for consultation. We will provide professional support and solutions tailored to your needs.
Related Service
O-glycosylation Analysis Service
Protein N-Glycosylation Analysis
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