Glycosylation Site Analysis Service

    The analysis of glycosylation sites in glycosylated proteins is of great importance for studying protein structure and function.

     

    MtoZ Biolabs provides a glycoform relative content analysis service based on LC-MS/MS. First, various enzymes are used to digest the protein, then in heavy-labeled H218O, PNGase F or various O-glycosidases are used to enzymatically release the glycans from the target protein. At this point, the glycosylated peptides will carry a 18O, and by searching the database, the glycosylation modification sites can be identified.

     

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    Figure 1. Glycosylation Site Analysis

     

    Service Advantages

    1. Relatively Short Identification and Analysis Time

    2. High Sensitivity and Good Reproducibility

     

    Applications

    1. Identification of N-Glycosylation Sites in Glycosylated Proteins

    2. Identification of O-Glycosylation Sites in Glycosylated Proteins

     

    Sample Submission Requirements

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    Figure 2. Glycosylation Site Analysis Sample Requirements

     

    1. If you provide tissue samples, please transport them to us on dry ice.

    2. If you provide protein samples, common tissue and cell lysis buffers can be used for protein extraction.

    3. Sample Transport: Please use an ample amount of dry ice and opt for the fastest possible mailing method to minimize sample degradation during transportation.

     

    Deliverables

    1. Experimental Procedures

    2. Relevant Mass Spectrometry Parameters

    3. Identification information for N-type and O-type glycosylation sites

    4. Mass Spectrometry Images

    5. Raw Data

     

    Services at MtoZ Biolabs

    1. N-glycosylation Site Analysis

    2. O-glycosylation Site Analysis

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