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    N-Glycan Profiling Services

      N-Glycans are complex carbohydrate chains covalently attached to the asparagine (Asn) residues of proteins. They participate extensively in key biological processes such as cell recognition, signal transduction, and immune regulation. Because their dynamic modification patterns can sensitively reflect disease states, drug responses, and aging processes, N-glycans hold great promise as biomarkers and targets for quality control in drug development. Built on a world-leading mass spectrometry platform, MtoZ Biolabs’ N-Glycan Profiling Services offer a one-stop solution that fully characterizes the composition, structure, and abundance of N-glycans from minimal sample volumes, helping researchers uncover the pivotal role of glycosylation in disease mechanisms, diagnostics, and therapies—and accelerating biomarker discovery and biopharmaceutical R&D.  

       

      1893953593972215808-n-glycan-profiling-service1.PNG

      Bangarh, R. et al. Biotechnol Adv. 2023.

      Figure 1. The Structure of N-Glycoproteins on the Cell Surface

       

      MtoZ Biolabs N-Glycan Profiling Services  

      MtoZ Biolabs provides a high-sensitivity, high-resolution, fully quantitative N-glycan profiling services, covering all scenarios from basic research to clinical diagnostics and biopharmaceutical development:

      1. Sample Compatibility: Serum/plasma, cell culture supernatants, monoclonal antibodies, recombinant proteins, etc.  

      2. Analytical Scope: Comprehensive profiling of core fucosylation, sialylation, branching patterns, and rare glycan types.  

      3. Technical Advantages: Capable of ultra-low-volume analysis (as little as 0.1 µL of serum or 10 ng of protein) and precise detection of α2,3/α2,6 sialic acid isomers—traditionally challenging for many techniques.

       

      Analysis Workflow  

      1. Sample Preparation  

      • Protein extraction and purification (removal of lipids and salts).  
      • Mild enzymatic cleavage (PNGase F) to release intact N-glycans, preventing artificial modifications such as desialylation.  

       

      2. Glycan Derivatization (Optional)  

      Fluorescent labeling of low-abundance glycans to enhance detection sensitivity.  

       

      3. High-Resolution Mass Spectrometry  

      Combined LC-MS/MS (liquid chromatography-tandem mass spectrometry) and CZE-MS (capillary zone electrophoresis-mass spectrometry) strategies for simultaneous determination of glycan composition, abundance, and isomeric variants.  

       

      4. Data Analysis  

      Database matching and multidimensional spectral interpretation to quantify target glycans, with comprehensive structural annotations and visual reports.  

       

      5. Customized Delivery  

      Differential glycan screening, biomarker candidate lists, pathway enrichment analyses (disease relevance).

       

      1893953128790347776-n-glycan-profiling-service2.PNG

      Figure 2. The Workflow of N-Glycan Profiling 

       

      Why Choose MtoZ Biolabs?  

      1. Industry-Leading Isomer Resolution  

      Single-run analysis distinguishes α2,3/α2,6 sialic acid linkages and resolves complex fucosyl branching.  

       

      2. Fully Quantitative & Highly Reproducible  

      Internal standards combined with multiple reaction monitoring (MRM) ensure cross-batch data consistency (CV < 8%).  

       

      3. End-to-End Customized Solutions  

      From cohort screening to biomarker validation, tailored to molecular mechanism exploration and translational medicine needs.  

       

      4. One-Time-Charge  

      Transparent pricing with no hidden fees or additional costs.

       

      Applications  

      1. Disease Biomarker Research  

      Detection of sialylated glycan biomarkers in neurological disorders (e.g., narcolepsy type 1).  

       

      2. Drug Development & Quality Control  

      • Glycosylation consistency assessment for monoclonal antibody drugs (e.g., PD-1 inhibitors) to optimize ADCC effects.  
      • Glycan profile comparison of biosimilars (core fucosylation, galactosylation rates).  

       

      3. Chronic Disease Studies  

      Analysis of aberrant glycosylation profiles in diabetes and cardiovascular diseases.

       

      Case Study  

      1. Highly-Sensitive Label-Free Deep Profiling of N-Glycans Released from Biomedically Relevant Samples  

      This study developed a highly sensitive, label-free method based on capillary zone electrophoresis-mass spectrometry (CZE-MS) for analyzing native N-glycans released from biomedically relevant samples. Compared to conventional CZE-MS approaches, this workflow achieves over a 45-fold increase in signal intensity. Qualitative and quantitative profiling of N-glycans in human serum IgG, bovine serum fetuin, bovine pancreatic RNase B, blood-derived extracellular vesicles, and total plasma resulted in the identification of >250, >400, >150, >310, and >520 N-glycans, respectively, with minimal sample requirements (as low as 0.2 nL plasma volume equivalent). Additionally, this method enables the identification of highly sialylated N-glycans and the differentiation of untreated sialic acid linkage isomers, facilitating high-sensitivity deep glycan analysis. The N-Glycan Profiling Services provide highly sensitive N-glycan analysis, capable of detecting and profiling N-glycans in various biological samples. It is applicable to proteomics, glycomics, and disease research, delivering precise data support for biomedical studies.

       

      1893953375650304000-n-glycan-profiling-service3.PNG

      Marie, AL. et al. Nat Commun. 2023.

      Figure 3. CZE-MS-Based N-Glycan Profiling of Biomedically Relevant Samples

       

      2. Non-Targeted N-Glycome Profiling Reveals Multiple Layers of Organ-Specific Diversity in Mice  

      This study presents a comprehensive N-glycome dataset from 20 different mouse tissues, demonstrating a multimodal data analysis workflow that provides deep and broad coverage of N-glycan features. The LC-MS/MS data-driven method integrates automated N-glycan identification, non-targeted profiling, and isomer-sensitive analysis. This approach revealed tissue-specific glycosylation patterns, non-canonical N-glycan structures, and highlighted the complex regulation of glycobiological pathways across organs. The research uncovers multiple layers of N-glycome complexity that are governed by organ-specific regulations. Our N-Glycan Profiling Services offer advanced N-glycan profiling, capable of analyzing complex glycosylation patterns in a wide range of biological samples. Using cutting-edge LC-MS/MS techniques, we provide deep and comprehensive N-glycome profiling, including isomer-sensitive glycan structural identification. It is ideal for uncovering tissue-specific glycosylation differences and exploring the regulatory complexity of glycobiological pathways in health and disease.

       

      1893953483670409216-n-glycan-profiling-service4.PNG

         Helm, J. et al. Nat Commun. 2024.  

      Figure 4. Comparative N-Glycome Analysis

       

      Deliverables  

      ✅ Comprehensive qualitative/quantitative list of total N-glycans  

      ✅ Abundance of key glycan types, including sialylation and fucosylation  

      ✅ Bar chart/heatmap visualizations (group comparison of differences)  

      ✅ Isomeric structure verification (Linkage-specific Sialylation)  

      ✅ Single-cell/exosome N-glycan profiles  

      ✅ Combined O-glycosylation/phosphorylation multi-omics analysis  

       

      N-glycosylation is an indispensable regulator within the “dark matter” of life processes, and its complexity makes it easy for traditional techniques to miss critical information. MtoZ Biolabs leverages an international-standard instrumentation platform, an experienced glycoscience team, and a customer-focused service philosophy to help you overcome technical barriers and accurately capture the biological significance behind every glycan modification. Whether you aim to discover novel diagnostic biomarkers, optimize biopharmaceutical production, or decipher glycosylation’s molecular role in disease, the N-Glycan Profiling Services in MtoZ Biolabs stands as your most reliable partner. Contact us and let the “language” of glycans empower your research and medical translation!

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