Resources

Proteomics Databases



Metabolomics Databases

• • Protein Separation Based on SDS-PAGE: Principles, Applications, and Advantages in Protein Research

  Protein separation and purification are critical aspects of protein research, with SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) being a widely employed technique. SDS-PAGE leverages the properties of SDS to denature proteins and impart a negative charge, facilitating the separation of proteins based on their molecular weight in an electric field. Understanding the principles and applications of SDS-PAGE is essential for elucidating protein structure and function.

• • Comprehensive Overview of Labeled and Label-Free Quantitative Proteomics Methods

  1. Labeled Quantitative Proteomics SILAC Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a technique for labeling proteins with stable isotopes under cell culture conditions. The SILAC experiment involves incorporating light, medium, or heavy stable isotope-labeled essential amino acids, such as lysine (Lys) and arginine (Arg), into the growth medium. As cells grow, newly synthesized proteins incorporate these labeled amino acids, thus becoming tagged with stable isotopes.

• • Analysis of Sialic Acid Content

  Recombinant protein drugs, derived from animal and plant sources through biotechnology, are protein products with specific biological activities used in the prevention, treatment, and diagnosis of diseases in humans, animals, and plants. Compared to small molecule drugs, recombinant protein drugs offer high activity, high specificity, and low toxicity, making them highly attractive to researchers. They are currently widely applied in the treatment of tumors, autoimmune diseases, metabolic disorders, g......

• • Exploring Protein Post Translational Modifications Diversity

  Protein post-translational modifications (PTMs) constitute a significant field in protein biology. A protein can undergo a variety of different types of post-translational modifications, and these modifications can coexist or occur at different times and under different conditions. There is immense diversity in the types of PTMs, including but not limited to phosphorylation, ubiquitination, methylation, acetylation, glycosylation, among others.   Each modification has its specific functions such as re......

• • Glycosylation Site Analysis Workflow

  Glycosylation sites are specific amino acid residues on proteins that are modified by glycosylation. N-glycosylation and O-glycosylation are the most common types of glycosylation modifications. N-glycosylation occurs on amino acids containing nitrogen, usually asparagine (Asn) residues. N-glycosylation generally follows a specific sequence pattern, i.e., "N-X-S/T" (where X can be any amino acid except proline), i.e., asparagine residue followed by any amino acid, then serine (Ser) or threonine (Thr).......

• • How AI & Machine Learning Enhance Label-Free Proteomics Data Analysis Efficiency?

  In recent years, proteomics has emerged as a powerful tool for elucidating biological processes and disease mechanisms. Among various approaches, label-free quantitative proteomics (LFQ) has been widely adopted in both fundamental research and clinical applications, owing to its flexible experimental design and minimal sample requirements. However, LFQ faces substantial challenges, including complex data processing workflows, high computational demands, and susceptibility to multiple sources of variability.

• • How to Overcoming Reproducibility Issues in Label-Free Proteomics?

  Label-free quantitative proteomics has become widely adopted in studies of disease mechanisms, biomarker discovery, and drug target identification, owing to its high sample throughput, streamlined workflow, and the absence of costly labeling reagents. However, as large-scale studies continue to expand, researchers are increasingly recognizing that poor reproducibility remains one of the major limitations hindering the broader application of label-free quantification. This article systematically examines....

• • DIA vs. DDA in Label-Free Quantitative Proteomics: A Comparative Analysis

  Label-free quantitative proteomics is widely used to investigate dynamic changes in protein expression within biological systems and plays a critical role in areas such as disease mechanism elucidation, drug target discovery, and biomarker identification. Among label-free quantification strategies, Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA) are the two most commonly employed mass spectrometry acquisition approaches, each characterized by distinct technical features and suited...

• • Tools for Label-free Peptide Quantification

  Label-free quantitative proteomics (LFQ) has emerged as a crucial method for quantitative analysis in life science research, owing to its simplified sample preparation, broad applicability, and the absence of label-induced interference. Particularly in fields such as systems biology, disease mechanism investigation, biomarker discovery, and drug development, LFQ strategies are playing an increasingly prominent role. This paper systematically reviews the principles and characteristics of mainstream .......

• • Issues and Resolutions in Label-Free Quantitative Mass Spectrometry

  With ongoing advances in systems biology, label-free quantification (LFQ) proteomics has emerged as a mainstream strategy in life science research. This approach offers distinct advantages, such as eliminating the need for costly isotope labeling, enabling simpler workflows, and supporting high-throughput sample processing. In particular, LFQ based on mass spectrometry (MS) has been extensively adopted in applications such as biomarker discovery, disease mechanism investigation, and drug action analysis....