Resources

Proteomics Databases



Metabolomics Databases

• • Reading the N-Terminus by Cycles: Edman Sequencing for Protein and Peptide N-Terminal Confirmation

  Introduction Many protein characterization projects do not begin with a full primary structure question. They begin with a narrower but critical one: does the N-terminus match the intended design? A recombinant product may pass intact mass and peptide mapping yet still require direct N-terminal confirmation.

• • Blocked or Weak Edman Signals? Troubleshooting N-Terminal Sequencing Sample Prep and PTM Interference

  Introduction An Edman sequencing run can finish without delivering the N-terminal answer the project needs. The sequencer may complete multiple cycles, yet the first residues are weak, ambiguous, or absent entirely.

• • Monoclonal Antibody Disulfide Bond Characterization and Integrity Analysis

  Monoclonal antibodies (mAbs) are a cornerstone of modern biopharmaceutical development and therapeutic applications. Their structural integrity directly determines drug stability, safety, and biological activity. Disulfide bonds, as critical stabilizing elements of antibody higher-order structure, maintain the three-dimensional conformation and functional properties of antibodies. Accurate characterization of disulfide bond connectivity and integrity is essential for antibody development, manufacturin......

• • Single-Cell iTRAQ Proteomics

  In life science research, understanding the diversity and complexity of cellular functions is fundamental. However, most proteomics studies are conducted on bulk cell populations. Although such approaches provide averaged protein expression profiles, they obscure heterogeneity among individual cells. In recent years, the development of single-cell iTRAQ proteomics has provided a powerful strategy for resolving cellular heterogeneity, enabling the investigation of fine-scale regulatory mechanisms at th......

• • Antibody Diversity And Specificity Analysis vs ELISA: Method Selection and Research Use Cases

  Technical guide for Antibody Diversity And Specificity Analysis vs ELISA: Method Selection and Research Use Cases.

• • Antibody Diversity And Specificity Analysis Troubleshooting: Causes, Checks, and Fixes for Research Workflows

  Technical guide for antibody diversity and specificity analysis troubleshooting with workflow checks, de novo sequencing decision logic, and interpretation limits.

• • How to Plan an Antibody Protein Sequencing Project When the Antibody Gene Sequence Is Unknown

  Antibody protein sequencing is often requested when a functional monoclonal antibody exists, but the antibody gene sequence is unknown, missing, or no longer trusted. A legacy hybridoma may still produce IgG, a recombinant batch may bind as expected, and internal records may list clone names or assay data, yet the exact VH and VL sequences needed for expression design, rescue, or documentation were never archived.

• • Epitope vs Antigen: Method Selection and Research Use Cases

  Decision-focused technical guide for selecting between antigen identification, epitope mapping, and staged de novo sequencing workflows in antibody-reactive samples.

• • How to Improve Antibody Diversity And Specificity Analysis Optimization in Research Workflows

  A decision-focused guide for immunology and antibody teams on resolving ambiguous diversity and specificity conclusions with protein-level sequence interpretation and validation planning.

• • Antigen vs Epitope: Method Selection and Research Use Cases

  Method-selection guide comparing antigen-level identification, epitope mapping, and de novo sequencing pathways for sequence-uncertain research projects.