Resources

Proteomics Databases



Metabolomics Databases

• • What Are Targeted Post-Translational Modifications?

  Post-translational modifications (PTMs) are chemical alterations that occur to proteins after translation, including phosphorylation, acetylation, ubiquitination, and others. These modifications not only regulate protein activity, subcellular localization, and stability, but also exert substantial influence on signaling pathways, cellular differentiation, and disease processes. In proteomics, targeted post-translational modifications analysis refers to the selection of specific PTM classes and defined......

• • How to Apply Targeted Mass Spectrometry for PTM Verification?

  Protein post-translational modifications (PTMs) constitute fundamental regulatory mechanisms underlying essential biological processes, including cell fate determination, signal transduction, and metabolic homeostasis. Common PTMs include phosphorylation, acetylation, ubiquitination, and methylation. Although high-throughput mass spectrometry approaches, such as data-dependent acquisition (DDA) and data-independent acquisition (DIA), are widely used for the initial discovery of PTM sites, subsequent v......

• • How to Identify Protein–Protein Interactions Using Mass Spectrometry?

  Protein–protein interactions (PPIs) are central to biological processes such as cellular signal transduction, metabolic regulation, and chromatin remodeling. Identifying and characterizing protein interaction networks not only facilitates mechanistic understanding of cellular functions but also plays an important role in drug-target screening and studies of disease mechanisms. With the rapid advancement of mass spectrometry (MS) in proteomics, MS has become one of the most widely used and robust appro......

• • What Is the Workflow for Targeted PTM Analysis?

  Post-translational modifications (PTMs) participate extensively in fundamental biological processes, including cellular signal transduction, metabolic regulation, and gene expression. Their dynamic alterations often serve as important molecular indicators of disease onset, progression, and responses to therapy. With rapid advances in mass spectrometry (MS), targeted PTM analysis has become a powerful approach for elucidating regulatory mechanisms in complex biological systems. In contrast to global pr......

• • How to Improve Sensitivity in Targeted PTM Detection by Mass Spectrometry?

  Protein post-translational modifications (PTMs), including phosphorylation, acetylation, ubiquitination, and glycosylation, play pivotal roles in regulating signal transduction, cellular metabolism, and disease pathogenesis. Relative to unmodified proteins, PTM-bearing proteins/peptides are frequently low in abundance, exhibit heterogeneous modification sites, and may be chemically or enzymatically unstable; consequently, achieving high sensitivity and specificity remains a central challenge. Owing to......

• • Why Is Targeted PTM Analysis Essential in Proteomics Research?

  In modern life-science research, proteomics has evolved from cataloguing proteins toward elucidating how proteins are regulated and how they shape cellular states. A central theme in this transition is the systematic characterization of post-translational modifications (PTMs). Across basic biology, studies of disease mechanisms, and drug-target discovery, targeted PTM analysis (targeted PTM proteomics) has become an indispensable approach. This article explains why targeted PTM analysis is a major foc......

• • How to Validate Exosome Integrity After Purification?

  Exosomes are nanoscale vesicles generated through the endosomal pathway and are widely present in various biological fluids, including plasma, urine, saliva, and milk. They have attracted extensive interest due to their important roles in intercellular communication, disease biomarker discovery, and drug delivery. However, functional investigations of exosomes rely fundamentally on the preservation of their structural and biological integrity. Loss of integrity may introduce experimental bias and even......

• • How to Improve Exosome Purity Without Losing Yield?

  Exosomes are key mediators of intercellular communication and have, in recent years, demonstrated substantial potential in tumor biology, neurodegenerative diseases, liquid biopsy, and drug delivery. However, exosomal functions and associated analytical readouts can be readily confounded by contaminants, including free proteins, liposomes, cell debris, and other non-specific components, thereby creating major obstacles for downstream omics analyses, functional validation, and clinical translation. In ......

• • How to Purify Exosomes Effectively: Comparing Ultracentrifugation, SEC, and Immunoaffinity Methods

  Exosomes are small vesicles released by diverse cell types and are commonly found in plasma, urine, saliva, milk, and cell-culture supernatants. Serving as key mediators of intercellular communication, exosomes have attracted sustained interest from both academia and industry in recent years, particularly for early cancer detection, disease biomarker discovery, and the development of delivery systems. Nevertheless, because exosomes are small (30–150 nm) and share highly overlapping physical characteri......

• • Glycoproteomics in Drug Development

  With the rapid advancement of precision medicine and the biopharmaceutical industry, glycoproteomics, as a cutting-edge technology for the systematic investigation of protein glycosylation, has emerged as an integral component of modern drug development. Its broad applications in target identification, biomarker discovery, and quality control of biopharmaceutical products provide high-resolution molecular insights that substantially facilitate innovative drug research and development. Overview of Gly......