Resources

Proteomics Databases



Metabolomics Databases

• • Affinity Pull-Down Assay

  The affinity pull-down assay is a biochemical technique that relies on specific molecular interactions. This method employs a known ligand (such as an antibody, small molecule, protein, or nucleic acid) immobilized on a solid-phase carrier (e.g., agarose or magnetic beads) to selectively bind the target protein in a sample. Following a series of stringent washing steps to remove non-specifically bound molecules, the target protein is ultimately eluted and enriched. Subsequent analysis using mass .......

• • Disease Target Identification

  Disease target identification is a critical process in elucidating disease pathogenesis and designing novel therapeutic strategies. Disease targets are commonly defined as biomolecules that play a pivotal role in disease progression, including proteins, RNA, and gene mutations, which can serve as direct points of drug intervention. Systematic screening and validation of disease-associated targets provide a scientific foundation for drug development, enhancing therapeutic accuracy and effectiveness. Beyond..

• • AP-MS Protein Interaction

  Affinity purification-mass spectrometry (AP-MS) protein interaction analysis is a widely used proteomics technique for investigating intracellular protein interaction networks. This method integrates affinity purification with mass spectrometry analysis to selectively isolate target proteins and their interacting partners using affinity-based capture, followed by identification and quantification through high-resolution mass spectrometry. This approach enables a systematic characterization of protein ......

• • Drug Target Identification Analysis

  Drug target identification analysis is the process of identifying key molecules that specifically bind to drugs and regulate biological functions using biotechnological and computational approaches. Drug targets are predominantly proteins, including receptors, enzymes, ion channels, and transcription factors, which are involved in disease onset and progression. By identifying drug targets, researchers can pinpoint disease-associated proteins and assess their potential as therapeutic targets, thereby .......

• • Pull-Down Assays

  The pull-down assay is a biochemical technique used to study protein-protein interactions and is widely utilized in molecular biology, cell biology, and biomedical research. This method relies on the specific binding between an affinity tag on a fusion protein and its corresponding immobilized ligand. To perform the assay, researchers first prepare a bait protein tagged with a specific affinity label and immobilize it on a solid-phase affinity matrix. Common affinity tag systems include GST (Glutathione....

• • Phylogenetic Analysis of Protein Sequences

  Phylogenetic analysis of protein sequences involves comparing protein sequences from different species or gene families to infer evolutionary relationships. This approach helps elucidate functional changes in proteins and the mechanisms underlying their adaptation during evolution. Initially, phylogenetic analysis was primarily used in taxonomy, where evolutionary trees were constructed based on morphological characteristics. However, with advancements in molecular biology, genetic and protein sequence.....

• • Single-Cell Proteomics Mass Spectrometry

  Single-cell proteomics mass spectrometry is a mass spectrometry-based approach designed to characterize the protein composition and dynamic changes within individual cells. Conventional proteomics techniques typically require large cell populations, whereas single-cell proteomics mass spectrometry overcomes this limitation by enabling the analysis of protein expression levels, post-translational modifications, and protein-protein interactions at single-cell resolution. This technology provides an .......

• • Protein Identification by Peptide Mass Fingerprinting

  Protein identification by peptide mass fingerprinting is a mass spectrometry-based approach for efficient protein identification. This method involves enzymatic cleavage of proteins into specific peptides, followed by mass spectrometric analysis to generate a unique peptide mass fingerprint. The resulting fingerprint is then compared with theoretical peptide mass data from protein databases to determine protein identity. Due to its high throughput, rapid analysis, and relatively simple experimental ........

• • Glycan Analysis Mass Spectrometry

  Glycan analysis mass spectrometry is a highly sensitive analytical technique employed to elucidate the structural characteristics and variations of glycans. Glycans are ubiquitous in biological systems and play essential roles in various biological processes, including cell signaling, immune response, protein folding, and cell-cell interactions. Compared to proteins and nucleic acids, glycans exhibit remarkable structural complexity and heterogeneity. Traditional glycan analysis techniques often struggle...

• • PY-GC-MS Analysis

  PY-GC-MS Analysis is an analytical technique that integrates pyrolysis, gas chromatography (GC), and mass spectrometry (MS). This method enables efficient and precise analysis of complex organic compounds, leading to its widespread application in various scientific and technological fields. In materials science, PY-GC-MS Analysis is extensively utilized to investigate the composition and degradation behavior of polymers. By analyzing the decomposition products, researchers can infer structural information..