What Detector Is Used for Mass Spectrometry in Proteomics
Mass spectrometry has emerged as a key research area in proteomics. The identification, quantification, and analysis of proteins in complex biological samples rely on mass spectrometry. Within mass spectrometry applications, the selection of an appropriate detector is particularly critical.
Electron Multiplier
The electron multiplier is a traditional detector in mass spectrometry, known for its high sensitivity through signal amplification via electron multiplication. It utilizes a high-voltage electric field to induce ions striking the detector to emit a cascade of secondary electrons, thereby amplifying the signal. However, the electron multiplier's linear dynamic range is limited due to space charge effects.
Reflectron Time-of-Flight Mass Spectrometer (Reflectron-TOF)
The Reflectron-TOF is a widely used time-of-flight detector, renowned for its extraordinary mass resolution. The electrostatic reflectron corrects for the initial velocity distribution of ions, enhancing resolution. Nevertheless, Reflectron-TOF has a restricted mass range and cannot conduct tandem mass spectrometry.
Quadrupole and Ion Trap
Quadrupole mass spectrometers offer a broad mass range with commendable resolution, but they exhibit lower sensitivity. Ion trap mass spectrometers, capable of tandem mass spectrometry, provide valuable structural insights, though they are limited by lower mass resolution and range.
Selecting a mass spectrometry detector should be aligned with specific experimental goals. For detailed protein structural analysis, an ion trap mass spectrometer is ideal. For analyzing a broad mass range, a quadrupole is suitable, while for ultra-high mass resolution, a Reflectron-TOF is preferred. Each detector type has distinct advantages and limitations, making the appropriate selection critical for advancing proteomics research.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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