PCR Based Antibody Sequencing Service

    Although hybridoma cells are immortalized, extensive passage of hybridoma cells may cause mutations on antibody genes, and hybridomas may loss their specificity to produce the correct antibody. Therefore, sequence information of antibody is of extreme importance for stable quality of antibody in large-scale industrial manufacturing. To solve this problem, MtoZ Biolabs has developed a PCR based antibody sequencing workflow, coupled with degenerate primer design. Compared with the mass spectrometry-based antibody sequencing method, this PCR-based antibody sequencing method is much more cost effective and less time consuming. In this method, degenerate FR1 primers are designed for PCR amplification of immunoglobulin (Ig) light and heavy chain variable regions from your hybridoma extracted mRNA. To better meet your research needs, we provide a full-package service, covering the whole analytical steps involving antibody sequencing. You can simply send us your hybridoma cells, and we will perform all the following analytical experiments.

     

    Analysis Workflow 

     

    MtoZ Biolabs provides accurate and cost effective antibody gene sequencing services, based on degenerate primer design and PCR amplification technology.

     

    Service Advantages 

    1. Sequencing of Antibodies Derived from Multiple Common Species, Including Human, Mice, Rat, etc

    2. Quick Turnaround Time

    3. High Quality Analysis with Competitive Price

    4. Full-Package Service and Custom Service Is Also Supported

     

    Sample Submission Requirements 

     

    Format Pellet of snap frozen hybridoma cells
    Quantity 1x10^7 cells

     

    Deliverables

    1. Amplified DNA Fragments of Light and Heavy Chain Variable Regions (Optional)

    2. Experimental Procedures and All Reagents

    3. DNA Sequencing Results

    4. DNA Sequence Alignments of Heavy and Light Chain Variable Regions

    5. DNA Gel Images

    6. Analytical Raw Data Files

     

    Related Services

    Protein Sequencing

    Protein De Novo Seq

    N-Terminal Sequencing

    C-Terminal Sequencing

    Edman Degradation

    Protein Full-Length Sequencing

     

    Protein Analysis

    Protein Identification

    Protein Mass Measurement

    PTMs Identification

     

    PTM Analysis

    Phospho-Proteomics

    Acetyl-Proteomics

    Ubiquitin-Proteomics

    Glyco-Proteomics

    Disulfide Bond

    Histone Modifications

Submit Inquiry
Name *
Email Address *
Phone Number
Inquiry Project
Project Description *

 

How to order?


/assets/images/icon/icon-message.png

Submit Inquiry

/assets/images/icon/icon-return.png