Membrane Protein-Based Drug Discovery Service
- Discovery of small-molecule drugs targeting membrane proteins.
- Development of membrane protein-targeting antibody therapeutics (e.g., anti-GPCR or anti-transporter monoclonal antibodies).
- Target validation for antibody–drug conjugates (ADCs).
- Vaccine antigen screening (e.g., targeting viral membrane proteins).
- Target exploration for rare or neurological diseases.
Membrane Protein-Based Drug Discovery Service refers to an integrated solution designed to support drug discovery efforts targeting membrane proteins. This service is built upon the high-fidelity expression, functional purification, and three-dimensional structural analysis of membrane proteins. It incorporates molecular interaction studies and high-throughput screening platforms to systematically validate drug targets and identify lead compounds, ultimately facilitating the development of small-molecule and antibody-based therapeutics.
Membrane proteins are abundantly located on the cell surface and play critical roles in signal transduction, substance transport, and intercellular communication. Due to their central involvement in these essential biological processes, they serve as key drug targets for a wide range of major diseases, including cancer, neurological disorders, and metabolic syndromes. Notably, more than 60% of clinically approved drugs act on membrane protein targets, with G protein-coupled receptors (GPCRs), ion channels, transporters, and receptor tyrosine kinases (RTKs) being the most common target classes. Conducting drug discovery research centered on membrane proteins enables structural-level understanding of target mechanisms, enhances screening efficiency, and improves the druggability of candidate compounds—ultimately accelerating the translation of novel therapeutics from bench to bedside.
Salazar GT. et al. Engineering. 2021.
Integrating structural biology, chemical biology, and drug screening methodologies, MtoZ Biolabs offers Membrane Protein-Based Drug Discovery Service that encompasses membrane protein expression and purification, structural analysis, interaction profiling, and high-throughput screening. This service is designed to accelerate the development of therapeutics targeting membrane proteins.
Analysis Workflow
The general technical workflow of Membrane Protein-Based Drug Discovery Service includes the following steps:
1. Target Identification and Gene Cloning
Define the membrane protein sequence and functional domains, followed by construction of the expression vector.
2. Membrane Protein Expression and Functional Validation
Express the target protein using a suitable system, and assess its membrane localization and biological activity.
3. Protein Extraction and Purification
Extract and purify the membrane protein using appropriate detergents or membrane-mimetic systems.
4. Structural Determination and Modeling
Resolve the 3D structure of the membrane protein using Cryo-EM or X-ray crystallography to guide structure-based drug design.
5. Drug Screening
Establish in vitro screening assays to identify potential small-molecule or antibody candidates.
6. Binding Validation and Affinity Measurement
Quantify molecular interactions using techniques such as SPR or mass spectrometry.
7. Drug candidate optimization recommendations
Provide structure-based and kinetics-guided optimization strategies for candidate therapeutics.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Membrane Protein-Based Drug Discovery Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Membrane Protein-Based Drug Discovery data, providing clients with a comprehensive data report.
4. Broad Target Compatibility: Supports a wide range of challenging membrane protein targets, including GPCRs, ion channels, transporters, and immune receptors.
5. Highly Integrated Platform: Covers the full workflow from expression and purification to structural analysis and interaction screening.
Sample Submission Suggestions
Sample submission recommendations should be determined based on the specific project. Please feel free to contact our technical support team prior to submission for tailored guidance.
Applications
Applications of Membrane Protein-Based Drug Discovery Service include, but are not limited to:
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Membrane and Membrane Protein Extraction Service
Membrane Protein Structure Determination Service
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