Hit Identification Service | Drug Discovery
- We accept a wide range of biological materials, including Cultured cells, tissues, body fluids, and other biological samples.
- Samples should be stored at -80℃ and shipped with dry ice to ensure the stability
A hit is defined as a molecule (small molecule, peptide, natural product, etc.) that demonstrates reproducible activity in a biological assay, with preliminary evidence of specificity and acceptable chemical tractability. Hit Identification lays the foundation for downstream lead optimization, candidate selection, and preclinical development. In the early stages of drug discovery, the ability to identify molecular entities that exhibit biological activity against a defined therapeutic target is a pivotal milestone.
Hit identification bridges the gap between abstract molecular concepts and actionable chemical matter. Whether derived from high-throughput screening (HTS), fragment-based drug discovery (FBDD), structure-based virtual screening, or phenotypic screening, validated hit compounds provide a concrete starting point for rational optimization. However, identifying truly promising hits requires an integrated strategy that combines robust assay development, intelligent screening libraries, data-driven triaging, and orthogonal validation.
Service at MtoZ Biolabs
MtoZ Biolabs offers comprehensive Hit Identification Services that support pharmaceutical companies, academic researchers, and biotech innovators in uncovering high-quality, validated hits.
Our services include:
1. DNA-Encoded Library Screening
We offer high-throughput DNA-encoded library screening to rapidly interrogate billions of compounds against protein targets, enabling efficient discovery of high-affinity binders with minimal material consumption.
2. High-Throughput & Fragment-Based Screening
Our HTS platform supports screening of large compound libraries and specialized fragment libraries using biochemical or cell-based assays optimized for sensitivity and scalability.
3. AI-Augmented Hit Discovery
Leveraging machine learning and predictive modeling, we provide in silico screening and compound prioritization to enhance hit diversity.
4. Biophysical Characterization
To confirm target engagement and binding kinetics, we apply orthogonal biophysical techniques including SPR, DSF, ITC, and NMR, ensuring reliable validation of true binders.
5. Structure-Enabled Hit Evaluation
We support hit progression through biostructural studies using X-ray crystallography and cryo-electron microscopy (Cryo-EM), offering direct insights into ligand binding modes and SAR potential.
6. Recombinant Protein Production
We provide expression and purification of assay-ready recombinant proteins, including wild-type and mutant constructs, tagged proteins, and co-complexes for binding or structural studies.
Figure 1. Hit Identification Strategies
Service Advantages
☑️Comprehensive Hit Discovery Platform: Integrated solutions spanning DEL, HTS, FBDD, AI screening, and phenotypic profiling—suitable for diverse target classes and project needs.
☑️Custom Assay Support: Customized biochemical and cell-based assays designed and optimized by experienced scientists to ensure high-quality screening performance.
☑️Data-Driven Decision Support: Insightful hit triaging and SAR recommendations supported by cheminformatics, structural clustering, and machine learning.
☑️One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
*Note: If you have specific requirements or need guidance on sample preparation, please do not hesitate to contact us.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Data Analysis, Preprocessing, and Estimation (project-dependent)
4. Raw Data Files
5. Detailed Hit Identification Results
Related Services
Structure-Based Drug Discovery Service
How to order?
